FastQCFastQC Report
Wed 6 Sept 2023
SRR12763773.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12763773.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153162469
Total Bases7.8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGGCGACGATACTCTGGGGGTTGCCCCACGGCAGATCGGAAGAGCACA101941526.655776749067685No Hit
CGCGGGATAGAGCAGTCTGGTAGCTCGTCGGGAGATCGGAAGAGCACACG55990863.6556514376899996No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCTAGAAGATCGGAAGAGCACACG55369793.615101686562652No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCTAGATCGGAAGAGCACACGTCT38455012.510733226688844Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGGCGACGATACTCTGGGGGTTGCCCCACGGCACAGATCGGAAGAGCA25055151.6358544076486519No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAGAAGATCGGAAGAGCACACG22304751.4562803894209881No Hit
AGCGGCGACGATACTCTGGGGGTTGCCCCACGGTAGATCGGAAGAGCACA20906681.365000194662571No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAGATCGGAAGAGCACACGTCT20757311.3552478055182042Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCAGGATGTCGGCGGTTCGAGCCCGTCATCGCCCAGATCGGAAGAGCAC19721731.2876346358715332No Hit
GGGGCTATAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT17339241.132081515348254TruSeq Adapter, Index 8 (100% over 36bp)
GCGGACGTGGCGGAATTGGTAGACGCGCTAGATTAGATCGGAAGAGCACA13427530.876685397386745No Hit
CGCGGGATAGAGCAGTCTGGTAGCTCGTCGGGCTCAGATCGGAAGAGCAC11528420.7526922277545682No Hit
AGCGGCGACGATACTCTGGGGGTTGCCCCACAGATCGGAAGAGCACACGT11041630.7209096374647761No Hit
GGGTCTGTAGTTCAATTGGTTAGAGCAGATCGGAAGAGCACACGTCTGAA10369070.6769980967073599Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGAGCGGTGGAAACTGCAAGACTAGAGTAGATCGGAAGAGCACACGTCT9926160.648080438034725Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCAGGATGTCGGCGGTTCGAGCCCGTCATCGCCTAGATCGGAAGAGCAC8411370.5491795774067862No Hit
GGGCTTGTAGCTTAGTGGATTAGAGCGCGTGAGATCGGAAGAGCACACGT8084040.5278081538369559No Hit
AGCAGGATGTCGGCGGTTCGAGCCCGTCATCGCCCACCAGATCGGAAGAG7032540.45915556506209104No Hit
GCCCCCATCGTCTAGAGGCCTAGGACACCTCCCTAGATCGGAAGAGCACA6940250.4531299374652938No Hit
GGGCTTGTAGCTTAGTGGATTAGAGCGCGTGGAGATCGGAAGAGCACACG6368010.4157683041790088No Hit
GGGTCTGTAGTTCAATTGGTTAGAGCACCGCCAGATCGGAAGAGCACACG6144700.40118836162141No Hit
GACAGCGGCGACGATACTCTGGGGGTTGCCCCACGGCAGATCGGAAGAGC5933710.3874127936655291No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCAGATCGGAAGAGCACACGTCTG5736850.3745597754760665Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCTATAGCTCAGTTGGTAGAGCACTTGCATGGCAAGATCGGAAGAGC5614860.3665950305358423No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAGATTTAGATCGGAAGAGCAC5602070.3657599695653901No Hit
CGCGGGATAGAGCAGTCTGGTAGCTCGTCGGAAGATCGGAAGAGCACACG4972420.3246500289832753No Hit
GGGGCTATAGCTCAGTTGGTAGAGCACTTGCAAGATCGGAAGAGCACACG4901330.32000855248683674No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCAGATCGGAAGAGCACACGTCTG4860740.3173584254508198Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTTGGTGTCTTTAGCGTCATGGAACCACTCCGATCCCAGATCGGAAGAG4705130.30719862579389473No Hit
GGGCTTGTAGCTTAGTGGATTAGAGCGCGTGGCAGATCGGAAGAGCACAC4697410.3066945858648962No Hit
GCCCCCATCGTCTAGAGGCCTAGGACACCTCCAGATCGGAAGAGCACACG4646800.3033902515635211No Hit
ACCGATTGGTCTTGGGTTCGAATCCCAGGCGACCCACCAGATCGGAAGAG4587190.299498305945956No Hit
AGAGTAGAAGTCTTTCAAACTATTGCCTAGGCGAGGTAGATCGGAAGAGC4577670.2988767437537195No Hit
GGGCTTGTAGCTTAGTGGATTAGAGCGCGTAGATCGGAAGAGCACACGTC4557100.29753372544549406No Hit
GGGCTTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT4525660.2954810032476037TruSeq Adapter, Index 8 (100% over 36bp)
GCGGATGTGGCGGAATTGGTATACGCGCAGATCGGAAGAGCACACGTCTG4507030.29426464782309036Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGGCGTAGCGCAGCTTGGTAGCGCACCACTTTGGAGATCGGAAGAGCA4435420.2895892204506053No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG4206830.2746645459208418TruSeq Adapter, Index 8 (100% over 49bp)
CGCGGGATAGAGCAGTCTGGTAGCTCGTCGGGCTAGATCGGAAGAGCACA4002420.261318587127242No Hit
GGCGACGATACTCTGGGGGTTGCCCCACGGCAGATCGGAAGAGCACACGT3927280.2564126855385179No Hit
ACGGAGGCGACAGGGATTCGAATTCCCTTGGGGGAGATCGGAAGAGCACA3909180.2552309338915136No Hit
CGGGATGTAGCGCAGCTTGGTAGCGCGCCTGCAGATCGGAAGAGCACACG3829990.250060607210504No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCCAGATCGGAAGAGCACACGTCT3808840.24867972061745755Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGGACGTGGCGGAATTGGTAGACGCGCTAGATAGATCGGAAGAGCACAC3591860.23451306468557906No Hit
GGGCGGCTGGCGGAATTGGTAGACGCAGATCGGAAGAGCACACGTCTGAA3465680.22627475403259525Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGCTATAGCTCAGTTGGTAGAGCACTTGCAGATCGGAAGAGCACACGT3394260.22161173178789642No Hit
ACCACGAGGTCGGGGGTTCGAGTCCCTCCAAGCCCGCCAGATCGGAAGAG3261050.212914431406822No Hit
GAGGTTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT3256320.21260560901509104TruSeq Adapter, Index 8 (100% over 36bp)
GCCCCCATCGTCTAGAGGCCTAGGACACCTCCCTTTCAGATCGGAAGAGC3140100.20501758821869082No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCTAGATTCAGATCGGAAGAGCAC3093950.202004447969561No Hit
GGGTCTGTAGTTCAATTGGTTAGAGCACCGCCCTGTAGATCGGAAGAGCA3068020.20031147447747136No Hit
GGGGCTATAGCTCAGTTGGTAGAGCACTTGCATGGCAGATCGGAAGAGCA3036530.1982554877722688No Hit
AGCGGCGACGATACTCTGGGGGTTGCCCCACGGCATAGATCGGAAGAGCA2895600.18905414746219584No Hit
GGGTCTGTAGTTCAATTGGTTAGAGCACCGCCCTGAGATCGGAAGAGCAC2846920.18587582314307038No Hit
TAAGGTCACGGGAAGACTACCCGTTGATAGGCTCTAGATCGGAAGAGCAC2802930.18300370960982615No Hit
GCGGGATAGAGCAGTCTGGTAGCTCGTCGGGAGATCGGAAGAGCACACGT2563010.16733929772312564No Hit
ATAGGAGCGGTGGAAACTGCAAGACTAGAGTAGATCGGAAGAGCACACGT2549370.16644874012836658No Hit
CACGGCGGAAGTTGCGGGTTCGAGCCCCGTCAGATCGGAAGAGCACACGT2530140.16519321061610726No Hit
GCCGGGATAGCTCAGTTGGTAGAGCAGTGGACTAGATCGGAAGAGCACAC2520930.16459188836920616No Hit
GCCCCCATCGTCTAGAGGCCTAGGACACCTCCCTTAGATCGGAAGAGCAC2362350.15423817697793837No Hit
ACTCGGTTGTGAAATGTGACAGCGGCGACGATAGATCGGAAGAGCACACG2316510.15124527667414397No Hit
GGGTCTGTAGTTCAATTGGTTAGAGCACCAGATCGGAAGAGCACACGTCT2313670.15105985265881292Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCAAGGGGTCAGCGGTTCGAGCCCGCTTAGCTCCACCAGATCGGAAGAG2289490.1494811369226491No Hit
GGGGAATTAGCTCAGTTGGTAGAGCGCTGCGAGATCGGAAGAGCACACGT2265400.14790829729964722No Hit
GCCCCCATCGTCTAGAGGCCTAGGACACCTCCCTTTAGATCGGAAGAGCA2249220.14685190273343007No Hit
CGGAGGCGACAGGGATTCGAATTCCCTTGGGGGTACCAGATCGGAAGAGC2237530.1460886609238455No Hit
CGGGGCGTAGCGCAGCTTGGTAGCGCACCACTTTGGGAGATCGGAAGAGC2226830.14539005635904184No Hit
GGCTTGGTAGCTCAGTTGGTTAGAGCAGGGGACTCAGATCGGAAGAGCAC2182900.14252186023457222No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCTAGATAGATCGGAAGAGCACAC2116510.1381872474254773No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCCAGATCGGAAGAGCACACGTCT2049680.13382390695203536Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGGACGTGGCGGAATTGGTAGACGCGCTAGAGATCGGAAGAGCACACGT2047220.13366329319227677No Hit
GCCCCCATCGTCTAGAGGCCTAGGACACCTCCCAGATCGGAAGAGCACAC2008290.1311215477990238No Hit
AGCAGGATGTCGGCGGTTCGAGCCCGTCATCGCCCACAGATCGGAAGAGC1969090.12856217406628515No Hit
GGGTCTGTAGTTCAATTGGTTAGAGCACCGCTAGATCGGAAGAGCACACG1913400.12492616582199391No Hit
GGGTCTGTAGTTCAATTGGTTAGAGTAGATCGGAAGAGCACACGTCTGAA1901960.12417924654897017Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGTCTGTAGTTCAATTGGTTAGAGCACAGATCGGAAGAGCACACGTCTG1901150.12412636153051307Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCAGGATGTCGGCGGTTCGAGCCCGTCATCGCCCACTAGATCGGAAGAG1889260.12335006169167982No Hit
ACTCGGTTGTGAAATGTGACAGCGGCGACGATACTAGATCGGAAGAGCAC1876040.12248692595834296No Hit
AGCTCAGTGGTAGAGCGGTCGGCTGTTAACCAGATCGGAAGAGCACACGT1799000.11745697309175657No Hit
CGCGGGATAGAGCAGTCTGGTAGCTCGTCGGAGATCGGAAGAGCACACGT1757170.11472588627439793No Hit
TCCGGTGTAGGCGTAAGAGTATTGAGAGGAGACTTCCTAGATCGGAAGAG1748530.11416177941085554No Hit
TCCTGAGGGTCGGGGGTTCGAATCCCTCCGTGTTCGCCAGATCGGAAGAG1731230.11303225988084588No Hit
GGGTCGATGCCCGAGTGGTTAATGGGGGCGGACTGTAGATCGGAAGAGCA1728010.11282202560994234No Hit
CAGCAGGATGTCGGCGGTTCGAGCCCGTCATCGCCCAGATCGGAAGAGCA1675710.10940735096141602No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCTAGAGATCGGAAGAGCACACGT1666550.10880929322182709No Hit
AGTAAGGTCACGGGAAGACTACCCGTTGATAGGCTCTAGATCGGAAGAGC1656430.10814855694184454No Hit
AAGGACTTCCGGGTTCAAGTCCCGGGTAGATCGGAAGAGCACACGTCTGA1618550.10567536620214707Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGGTTGTAGCTCAGACGGATAGAGCAAGCGCCAGATCGGAAGAGCACAC1616660.10555196782574719No Hit
CCACGAGGTCGGGGGTTCGAGTCCCTCCAAGCCCGCCAGATCGGAAGAGC1586440.10357889960627366No Hit
GGGTCTGTAGTTCAATTGGTTAGAGCACCGCAGATCGGAAGAGCACACGT1583120.10336213632074578No Hit
ACACGTGAGAACCTACCTTCAGAATGGGGAGATCGGAAGAGCACACGTCT1576050.10290053498680542Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCGTTGATAGGCTCTAGATGGAAGTCCAGTAGATCGGAAGAGCACACG1549210.10114814746163435No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)