FastQCFastQC Report
Tue 5 Sept 2023
SRR12764729_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764729_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18556130
Total Bases591 Mbp
Sequences flagged as poor quality0
Sequence length15-50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG5732543.089297175650311No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG4438152.3917433214792094No Hit
ATCGCATGATGAGTAATAGAAAGGAGCTT3887342.0949087983324106No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA1945211.0482843135934057No Hit
AGCGTTTACTTTGAAAAAATTA1794230.9669203653994664No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC1518540.818349515766488No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1436300.7740299297321155No Hit
CAGAGTTCTACAGTCCGACGATC1370640.7386453964269489Illumina DpnII expression Sequencing Primer (100% over 23bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1130910.6094535875745644No Hit
GTTCTACAGTCCGACGATC1077760.5808107617267179Illumina DpnII expression Sequencing Primer (100% over 19bp)
AGAGTTCTACAGTCCGACGATC1038900.5598688950767212Illumina DpnII expression Sequencing Primer (100% over 22bp)
CGACTGCATAATTTGTGGTAGTGGGGGA897540.48368921752542154No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTA890010.4796312593196965No Hit
TCTACAGTCCGACGATC887880.4784833906638938Illumina DpnII expression Sequencing Primer (100% over 17bp)
AACGATGCCGACTGGCGATGCGGCGGCGTT822400.4431958603437247No Hit
AGTTCTACAGTCCGACGATC789810.42563293100447125No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC695330.3747171419902749No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG684990.3691448594076459No Hit
TTCTACAGTCCGACGATC673760.3630929509547519Illumina DpnII expression Sequencing Primer (100% over 18bp)
TCGACTGCATAATTTGTGGTAGTGGGGG670870.36153551414007123No Hit
CTACAGTCCGACGATC653940.35241184449559254Illumina DpnII expression Sequencing Primer (100% over 16bp)
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG649600.35007299474621056No Hit
ACGATGCCGACTGGCGATGCGGCGGCGTTA645410.3478149808176597No Hit
GCGTTTACTTTGAAAAAATTA569490.3069012773676408No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG557700.3005475818503104No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA545710.2940861052385384No Hit
GGAAGATTAGCTCAGTTGGGAGAGCATCG530300.28578157191181564No Hit
ACGAATTGAATGATTTCATGTTTCCAAGAAAAGCTTC529630.2854205052454364No Hit
ATTTCACTAACGCAGCTAACGCGTTAAATGATCCGCCTGAGTA526490.2837283420627038No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG507360.2734190803793679No Hit
TTTCACTAACGCAGCTAACGCGTTAAATGATCCGCCTGAGTA478440.2578339341231172No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG473870.25537113611512746No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCG436580.23527535105649722No Hit
AACGGGACTCAAACACAATACCGAAGCTACGGGCACAT432550.23310356200349963No Hit
GAGTTCTACAGTCCGACGATC418360.22545649335287046Illumina DpnII expression Sequencing Primer (100% over 21bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT414660.22346254310570143No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCT408950.22038539285939468No Hit
TATCAACCTGTACCGAGAACGAACACACGTTCCCAA400260.21570230430590864No Hit
TACAGTCCGACGATC398350.21467299485399163Illumina DpnII expression Sequencing Primer (100% over 15bp)
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTA387950.20906837794303015No Hit
GACTGCATAATTTGTGGTAGTGGGGGA368770.19873217098608384No Hit
CGACTGCATAATTTGTGGTAGTGGGGG365660.19705617496751748No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCT353280.19038452522158444No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTG352250.1898294525852104No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTC348640.1878840038305401No Hit
ACGATGCCGACTGGCGATGCGGCGGCGTT342190.18440806353479955No Hit
CCCCACAACCGCGTTTGACTAGCTTGACATTTT341280.18391765955509043No Hit
GAAGCGTTTACTTTGAAAAAATTA332300.17907828841466406No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG309720.1669098028522111No Hit
ACGAATTGAATGATTTCATGTTTCCAAGAAAAGCTT306330.16508291330142652No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTAT301860.16267400584065753No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT297700.1604321590762729No Hit
CCCCACAACCGCGTTTGACTAGCTTGACATTT283490.15277431231619956No Hit
GACGCTAGTTCTAGTGGAGTCAACCTTGAAATACCACCCTT282750.15237552226676573No Hit
AACGGGACTCAAACACAATACCGAAGCTACGGGCACA278450.15005822873627206No Hit
GGAGTGGACGTACCGCTGGTGTTCCAGTT260070.14015314615709204No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC256540.13825080984019836No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCC253390.13655325760274367No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGC252860.13626763770247352No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGT251670.1356263401905462No Hit
GTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCT248700.13402579093808892No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCC241360.1300702247720834No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC240330.12951515213570933No Hit
AAGCGTTTACTTTGAAAAAATTA240320.12950976308098724No Hit
GTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTC233670.12592604169080512No Hit
TAACGGGACTCAAACACAATACCGAAGCTACGGGCACAT233270.12571047950192202No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT219240.11814963572684607No Hit
TATCAACCTGTACCGAGAACGAACACACGTTCCCA218320.11765384269241486No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCC213400.11500242776915229No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTC211090.11375755612835219No Hit
AACGATGCCGACTGGCGATGCGGCGGCG196000.10562547255273595No Hit
GGAGTGGACGTACCGCTGGTGTTCCAGT192610.10379858300195138No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC192490.10373391434528643No Hit
AACGATGCCGACTGGCGATGCGGCGGCGT189940.10235970539115645No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCT187690.10114716807868881No Hit
CCCCCGGCCCGCCCCCGG186190.10033880987037706No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)