FastQCFastQC Report
Tue 5 Sept 2023
SRR12764730_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764730_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15268068
Total Bases494.9 Mbp
Sequences flagged as poor quality0
Sequence length15-50
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG5560923.6421896994433087No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG4747483.109417642101149No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA2683861.7578255480654135No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC2408991.5777962214996684No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1684411.1032240621406717No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA1614491.0574291390371067No Hit
CAGAGTTCTACAGTCCGACGATC1194330.7822404249182019Illumina DpnII expression Sequencing Primer (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC1028960.673929406130494No Hit
GTTCTACAGTCCGACGATC991000.6490670594341078Illumina DpnII expression Sequencing Primer (100% over 19bp)
TCGACTGCATAATTTGTGGTAGTGGGGG978870.64112237383276No Hit
AGAGTTCTACAGTCCGACGATC971960.6365965883830227Illumina DpnII expression Sequencing Primer (100% over 22bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT914720.599106579823983No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGT763330.4999519258101287No Hit
TCTACAGTCCGACGATC745270.48812331723961405Illumina DpnII expression Sequencing Primer (100% over 17bp)
AGTTCTACAGTCCGACGATC718730.47074063332701954No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG717590.46999397697207007No Hit
CGACTGCATAATTTGTGGTAGTGGGGG644280.4219787336551029No Hit
ATCGCATGATGAGTAATAGAAAGGAGCTT625820.40988814039864113No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC618500.40509382064580796No Hit
TTCTACAGTCCGACGATC600420.39325211284099604Illumina DpnII expression Sequencing Primer (100% over 18bp)
AACGATGCCGACTGGCGATGCGGCGGCGTTA578510.3789019016682399No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG556990.36480712556428224No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG556520.3644992935582943No Hit
GACTGCATAATTTGTGGTAGTGGGGGA521890.3418179693724183No Hit
ACGATGCCGACTGGCGATGCGGCGGCGTTA508920.3333231159305814No Hit
CTACAGTCCGACGATC505650.3311813911229633Illumina DpnII expression Sequencing Primer (100% over 16bp)
AACGATGCCGACTGGCGATGCGGCGGCGTT504470.33040853629941913No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT474240.31060904365896197No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT432840.2834936286634301No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG418040.2738001952833849No Hit
GGAAGATTAGCTCAGTTGGGAGAGCATCG394750.2585461369441111No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC381030.2495600622161232No Hit
GAGTTCTACAGTCCGACGATC378570.24794885639754816Illumina DpnII expression Sequencing Primer (100% over 21bp)
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCG377570.24729389468268023No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTA357320.23403091995660485No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG346730.2270948753961536No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCC340890.22326989898132496No Hit
ACGATGCCGACTGGCGATGCGGCGGCGTT340290.2228769219524042No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTC334310.21896025089749405No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAA316640.2073870773957779No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG309870.20295298658612207No Hit
CCCCACAACCGCGTTTGACTAGCTTGACATTTT309820.2029202385003787No Hit
ATTTCACTAACGCAGCTAACGCGTTAAATGATCCGCCTGAGTA302070.19784428521015235No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG299020.19584665197980516No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAT296100.19393416377239084No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT289510.18961796607141126No Hit
TACAGTCCGACGATC283700.18581263850802865Illumina DpnII expression Sequencing Primer (100% over 15bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGG281240.18420143268945358No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC271810.1780251437182491No Hit
CCCCACAACCGCGTTTGACTAGCTTGACATTT270370.1770819988488393No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCC254490.16668120681673673No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTA252580.165430229941339No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTG250310.16394346684858882No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA247550.1621357725155534No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC235980.15455786547453157No Hit
GACTGCATAATTTGTGGTAGTGGGGG226620.14842742382336782No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT218440.14306983699574827No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCC201110.13171935047708722No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG197760.12952522873227967No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGT197330.12924359519488648No Hit
TCGACTGCAAAATTTGTGGTAGTGGGGGA192200.12588364159761406No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC185050.12120066533630844No Hit
AGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCC183650.12028371893549335No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG178100.1166486814179764No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA176780.11578413195435075No Hit
TATCAACCTGTACCGAGAACGAACACACGTTCCCAA175520.11495888019361715No Hit
CCCCCCGCGGGGGCGCGCCGGC174290.11415327728432963No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTT168950.11065578172693494No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC165780.10857955309080364No Hit
AACGATGCCGACTGGCGATGCGGCGGCGT161670.10588766044269649No Hit
GGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC161650.10587456120839912No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTC158260.10365424099499689No Hit
GTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCG157880.10340535554334708No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT156940.10278969153137124No Hit
TCGACTGCATAATTTGTGGTAGTGGGG155680.10196443977063764No Hit
ACGAATTGAATGATTTCATGTTTCCAAGAAAAGCTTC153600.10060211940371237No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGT152700.10001265386033124No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)