FastQCFastQC Report
Tue 5 Sept 2023
SRR12764731_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764731_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22968629
Total Bases767.8 Mbp
Sequences flagged as poor quality0
Sequence length15-50
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG17890067.7889106920574145No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG11446864.983693193006862No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA5494682.3922542351134672No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC4106751.787982208254572No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA3332291.450800568026938No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC3305571.4391673094637036No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC2604291.1338465173519936No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG2424431.0555397102717798No Hit
CAGAGTTCTACAGTCCGACGATC1787500.7782353922822298Illumina DpnII expression Sequencing Primer (100% over 23bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG1773250.7720312779661337No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG1772840.7718527736244074No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1741310.7581253543692138No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1731890.7540241082739418No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1688740.7352376147483597No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGT1476640.6428942711382556No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT1466090.6383010496621283No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG1430010.6225926675902163No Hit
AGAGTTCTACAGTCCGACGATC1398330.6087999418685373Illumina DpnII expression Sequencing Primer (100% over 22bp)
GTTCTACAGTCCGACGATC1284380.5591887961619303Illumina DpnII expression Sequencing Primer (100% over 19bp)
CGACTGCATAATTTGTGGTAGTGGGGG1170460.5095907117486203No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC944220.41109114523117596No Hit
AGTTCTACAGTCCGACGATC916580.39905734034016577No Hit
TCTACAGTCCGACGATC915500.3985871337814721Illumina DpnII expression Sequencing Primer (100% over 17bp)
GACTGCATAATTTGTGGTAGTGGGGGA864240.37626973730125557No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT783310.34103472175026206No Hit
CCGCCGAGGGCGCACCACCGGCCCGTC768680.33466516438573674No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTT717240.3122694001457379No Hit
TTCTACAGTCCGACGATC709620.3089518316482886Illumina DpnII expression Sequencing Primer (100% over 18bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCC670610.29196779659769856No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGG616940.26860114288928605No Hit
CTACAGTCCGACGATC605610.26366832778743565Illumina DpnII expression Sequencing Primer (100% over 16bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC598370.2605162023384156No Hit
CCCCACAACCGCGTTTGACTAGCTTGACATTTT575570.25058961943266184No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCC543910.23680560123984762No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAT541320.23567797625186948No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAA538760.23456341255718832No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCC533860.23243006798533772No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTA531880.23156802262773282No Hit
CCCCACAACCGCGTTTGACTAGCTTGACATTT528940.23028801588462244No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTA525610.22883821232865054No Hit
ACGATGCCGACTGGCGATGCGGCGGCGTTA518970.22594731274557137No Hit
GAGTTCTACAGTCCGACGATC518270.225642549235307Illumina DpnII expression Sequencing Primer (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGA494460.21527623612188607No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG472210.20558911025991147No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCC450000.19591939945566625No Hit
CCCCCCGCGGGGGCGCGCCGGC419300.18255334264835746No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT417300.18168258976188784No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGT393700.17140770570154623No Hit
GTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTC385340.1677679586361032No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCT377590.1643937912010334No Hit
AGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCC370630.16136357115611905No Hit
TCGACTGCAAAATTTGTGGTAGTGGGGGA369400.16082805813094025No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTA365410.15909090612243335No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT363150.15810695536072264No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC362130.15766287138862314No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC358590.15612163877957191No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC345390.15037466972887237No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC343310.14946908672694395No Hit
GACTGCATAATTTGTGGTAGTGGGGG339380.14775805730503114No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG339380.14775805730503114No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTG338680.14745329379476677No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC336490.14649981938408252No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTT331250.1442184468215321No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT329950.14365245744532684No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG320440.13951202747016375No Hit
ACGATGCCGACTGGCGATGCGGCGGCGTT319870.13926386289751994No Hit
TACAGTCCGACGATC317560.1382581433136475Illumina DpnII expression Sequencing Primer (100% over 15bp)
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATA306340.13337321962055287No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGG305850.13315988516336782No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTT300820.1309699416538967No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC291160.1267642052122484No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC289670.12611549431182853No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGT287210.1250444682614709No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTC280330.12204907833201538No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCG279080.12150485777797186No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCT269790.11746021062032043No Hit
AGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGTG266280.11593203930456623No Hit
GCTGGTCCGATGGTAGTGGGTTATCAGAA259460.1129627719617048No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT257190.11197446743556178No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCG254170.11065963057699263No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTT253790.11049418752856342No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC252100.10975840133949659No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCC252060.10974098628176718No Hit
CGACTGCAAAATTTGTGGTAGTGGGGGA251550.10951894429571743No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATA245570.10691539316517325No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT245160.10673688882344697No Hit
GTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCT243480.10600545639881248No Hit
TCGACTGCATAATTTGTGGTAGTGGGG239990.10448599261192298No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT239560.10429878074133202No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA238430.10380680536047668No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGG238180.10369796124966796No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT236830.10311020305130097No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGT236690.1030492503492481No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCT234250.10198693182775515No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)