FastQCFastQC Report
Tue 5 Sept 2023
SRR12764732_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764732_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27048479
Total Bases851 Mbp
Sequences flagged as poor quality0
Sequence length15-50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG10624863.9280803922468244No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG5531162.044906110986869No Hit
CAGAGTTCTACAGTCCGACGATC4758861.7593817382485721Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGAGTTCTACAGTCCGACGATC4063641.5023543468007943Illumina DpnII expression Sequencing Primer (100% over 22bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC3990541.4753287976007818No Hit
ATCGCATGATGAGTAATAGAAAGGAGCTT3986631.4738832449691535No Hit
GTTCTACAGTCCGACGATC3619031.337979115202744Illumina DpnII expression Sequencing Primer (100% over 19bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC3334831.2329085121570054No Hit
AGTTCTACAGTCCGACGATC2701770.9988620801931228No Hit
TCTACAGTCCGACGATC2700720.9984738883099489Illumina DpnII expression Sequencing Primer (100% over 17bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC2369910.8761712627168426No Hit
TTCTACAGTCCGACGATC2156270.7971871542203907Illumina DpnII expression Sequencing Primer (100% over 18bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT2047060.75681150130475No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA1992730.7367253441496655No Hit
CTACAGTCCGACGATC1915030.7079991447947961Illumina DpnII expression Sequencing Primer (100% over 16bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG1721660.6365089881763777No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG1716990.6347824585626423No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGT1698750.6280390109920784No Hit
GAGTTCTACAGTCCGACGATC1502620.5555284642807458Illumina DpnII expression Sequencing Primer (100% over 21bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT1410800.5215820083635757No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT1326570.4904416252019199No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA1324520.48968372676334226No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTT1305020.48247444893296954No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTA1231300.45521968166860693No Hit
ATTTCACTAACGCAGCTAACGCGTTAAATGATCCGCCTGAGTA1211680.44796603905158583No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1150040.42517732697650024No Hit
TACAGTCCGACGATC1117170.4130250725003798Illumina DpnII expression Sequencing Primer (100% over 15bp)
TCGACTGCATAATTTGTGGTAGTGGGGG1098530.4061337423076543No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1017500.3761764201232905No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG1012150.37419849005188055No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGG981090.36271540444104083No Hit
ACGATGCCGACTGGCGATGCGGCGGCGTTA978610.3617985321836396No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGG906360.33508723355572045No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT806420.29813876040867215No Hit
TTTCACTAACGCAGCTAACGCGTTAAATGATCCGCCTGAGTA796890.29461545693567465No Hit
CGACTGCATAATTTGTGGTAGTGGGGG788470.2915025277391753No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAT769090.2843376146954511No Hit
CTCCCGGGGCTACGCCTGTCTGAGCG738480.273020897034543No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAA730640.27012239764017787No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGG693220.2562879783369704No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC654930.2421319143305618No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG649690.24019465198024625No Hit
ACGATGCCGACTGGCGATGCGGCGGCGTT625880.2313919388960836No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT598810.22138398244130475No Hit
TATCAACCTGTACCGAGAACGAACACACGTTCCCAA577830.21362753890893457No Hit
ACGAATTGAATGATTTCATGTTTCCAAGAAAAGCTTC572670.21171985308305136No Hit
GACTGCATAATTTGTGGTAGTGGGGGA510240.18863907282919679No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT510050.18856882858367008No Hit
CAGAGTTCTACAGTCCGACGA501790.1855150524360353Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCCTCGGTTGGCCTCGGATAGCCGGTCCCC498740.18438744744205393No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCC495540.18320438646476203No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGC455770.16850115675635588No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGC440890.16299992321194845No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTT431590.1595616522466938No Hit
AGAGTTCTACAGTCCGACGA408260.1509363983091249No Hit
AACGATGCCGACTGGCGATGCGGCGGCG405410.14988273462622428No Hit
GTTCTACAGTCCGACGA392570.14513570245484045Illumina DpnII expression Sequencing Primer (100% over 17bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC390640.14442216880291125No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCC390130.14423361845965535No Hit
GACTGCATAATTTGTGGTAGTGGGGG374210.13834789009762805No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC363260.13429960331595725No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT360940.1334418841074206No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG360420.13324963669861067No Hit
ACGAATTGAATGATTTCATGTTTCCAAGAAAAGCTT353850.13082066462960817No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTT351100.12980397160224796No Hit
AACGGGACTCAAACACAATACCGAAGCTACGGGCACA347370.12842496615059204No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC346000.12791846816968896No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTA338660.1252048220530256No Hit
AGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCC338560.12516785139748524No Hit
GGAAGATTAGCTCAGTTGGGAGAGCATCG331400.12252075246079457No Hit
AACGATGCCGACTGGCGATGCGGCGGCGT322100.11908248149553992No Hit
GTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGC320050.11832458305696227No Hit
TCGACTGCATAATTTGTGGTAGTGGGG302910.11198781269734243No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA302600.11187320366516727No Hit
GGAGTGGACGTACCGCTGGTGTTCCAGTT301550.11148501178199337No Hit
GTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCT301280.11138519101203435No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCC301230.11136670568426417No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCC297300.10991375892152754No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT295140.10911519276185547No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCG294680.10894512774636976No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCC293990.1086900302231412No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGA292830.10826117061887289No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA291380.10772509611353748No Hit
TCTACAGTCCGACGA289830.1071520509526617Illumina DpnII expression Sequencing Primer (100% over 15bp)
CGGGCGGGTCGCGCCGTCGGGCCCGGGGG286650.10597638410647786No Hit
AGTTCTACAGTCCGACGA285780.10565473940327662Illumina DpnII expression Sequencing Primer (100% over 18bp)
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCT283730.10489684096469896No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT280690.10377293303627166No Hit
TATCAACCTGTACCGAGAACGAACACACGTTCCCA280220.1035991709552319No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC276900.10237174519129152No Hit
AACGGGACTCAAACACAATACCGAAGCTACGGGCACAT273850.10124414019731018No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATA272340.10068588329865055No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)