FastQCFastQC Report
Tue 5 Sept 2023
SRR12764733_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764733_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22153135
Total Bases714.8 Mbp
Sequences flagged as poor quality0
Sequence length15-50
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG9818914.43228915455984No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG5914162.6696718094301324No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA3452031.5582580072752683No Hit
GCCGGGCGCGGTGGCGCGT3102741.4005873209367434No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC2658831.200204846853504No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA2036540.9193010379795005No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1844250.8325006821833569No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG1780610.8037733711278336No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1750080.7899920259593055No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTA1712280.7729289782236238No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTT1698570.766740237894095No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1681870.7592018014606059No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG1499330.676802628612158No Hit
ATCGCATGATGAGTAATAGAAAGGAGCTT1370160.6184948541143274No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1251100.5647507677807226No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1138810.5140626823246461No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGT1103350.4980559184964114No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT1008910.45542538335996235No Hit
CGACTGCATAATTTGTGGTAGTGGGGG988640.44627543686254795No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG972300.43889950564558916No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC797610.3600438493242604No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC796740.3596511283843122No Hit
GACTGCATAATTTGTGGTAGTGGGGGA763340.3445742555173342No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT673530.3040337180268165No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT665190.3002690138438645No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG634320.28633419152639117No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT614140.27722487133310925No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCG601800.27165455363315394No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA585480.2642876504837803No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG582360.2628792719405177No Hit
TACAGTCCGACGATC569530.2570877665847294Illumina DpnII expression Sequencing Primer (100% over 15bp)
CCCCCCGCGGGGGCGCGCCGGC560230.25288971515769665No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG558860.25227129252812297No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTT531410.23988026976768753No Hit
ACGATGCCGACTGGCGATGCGGCGGCGTTA497450.22455061100832904No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTTA472350.21322038618913305No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCC457420.20648093373691806No Hit
GACTGCATAATTTGTGGTAGTGGGGG449140.2027433137567211No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTA439490.19838727114695054No Hit
AACGATGCCGACTGGCGATGCGGCGGCG427410.19293431832560043No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTT425930.19226624132430917No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTA418910.18909738960196829No Hit
CCCCACAACCGCGTTTGACTAGCTTGACATTTT411520.1857615186293046No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC410250.18518823633765605No Hit
CCCCACAACCGCGTTTGACTAGCTTGACATTT410190.185161152134901No Hit
AACGATGCCGACTGGCGATGCGGCGGCGT407690.1840326436867739No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT368990.1665633329097665No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG359470.16226597273929852No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC345130.1557928482808415No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGG339240.1531340823770541No Hit
AGCTTCACTTAAAAATGGGGGTGCGGAACA317720.14341988165557606No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT316730.14297299231011773No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA305380.13784956395562073No Hit
TCGACTGCAAAATTTGTGGTAGTGGGGGA303690.1370866922446868No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT300330.135569976890404No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCG299260.13508697527460561No Hit
ACGATGCCGACTGGCGATGCGGCGGCGTT299250.1350824612408131No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG296410.1338004756437407No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC295410.1333490722644899No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTATT295410.1333490722644899No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC295290.13329490385897977No Hit
TATCAACCTGTACCGAGAACGAACACACGTTCCCAA292620.13208965683638005No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC272820.12315186992721346No Hit
TCGACTGCATAATTTGTGGTAGTGGGG271590.12259664377073493No Hit
GCTGGTCCGATGGTAGTGGGTTATCAGAA263240.11882742555399044No Hit
AGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGTG259030.11692701732734442No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCGTTA256850.11594295796057759No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT254180.11473771093797784No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTT252990.11420054091666935No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATA252430.11394775502428889No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG252290.11388455855119377No Hit
GTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG250060.11287792901546441No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCGTT243850.11007471403031671No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTAT243010.10969553519174599No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT239120.10793957604646025No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAT237010.10698711491624097No Hit
CGGGCGGGTCGCGCCGTCGGGCCCGGGGG232710.10504608038546237No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATAC231480.10449085422898384No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC227300.10260398810371535No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC226160.10208938825136937No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCC223770.10101053417495989No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)