FastQCFastQC Report
Tue 5 Sept 2023
SRR12764734_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764734_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7932753
Total Bases236.8 Mbp
Sequences flagged as poor quality0
Sequence length15-50
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAGTTCTACAGTCCGACGATC4083205.1472672853926Illumina DpnII expression Sequencing Primer (100% over 23bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG4038035.09032614528651No Hit
AGAGTTCTACAGTCCGACGATC3182284.01157076238224Illumina DpnII expression Sequencing Primer (100% over 22bp)
GTTCTACAGTCCGACGATC3090833.896289220148415Illumina DpnII expression Sequencing Primer (100% over 19bp)
AGTTCTACAGTCCGACGATC2404293.0308393567781575No Hit
TCTACAGTCCGACGATC2268932.8602050259222747Illumina DpnII expression Sequencing Primer (100% over 17bp)
TTCTACAGTCCGACGATC1859652.3442681248237527Illumina DpnII expression Sequencing Primer (100% over 18bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1659482.091934540253554No Hit
CTACAGTCCGACGATC1477861.862985019198253Illumina DpnII expression Sequencing Primer (100% over 16bp)
GAGTTCTACAGTCCGACGATC1258041.5858807150556684Illumina DpnII expression Sequencing Primer (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC1238191.5608578762001035No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG836981.0550939881778747No Hit
TACAGTCCGACGATC773730.9753612648723589Illumina DpnII expression Sequencing Primer (100% over 15bp)
CGACTGCATAATTTGTGGTAGTGGGGGA580320.731549312073627No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG529640.6676622857159425No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA490190.6179317571087868No Hit
CGACTGCATAATTTGTGGTAGTGGGGG394860.49775910078127983No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG321270.40499181053538413No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG310000.3907848889282195No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG294720.37152297569330595No Hit
AGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTC247420.31189676522135507No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGC244690.30845533700595495No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC237860.29984546348537516No Hit
CAGAGTTCTACAGTCCACGATC219940.27725557571249226No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG195480.24642138737963984No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC194050.24461873450490643No Hit
CCCCACAACCGCGTTTGACTAGCTTGACATTT185610.23397930075473167No Hit
CCCCACAACCGCGTTTGACTAGCTTGACATTTT161910.20410316569796136No Hit
GACTGCATAATTTGTGGTAGTGGGGGA157980.19914902178348426No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCC152830.19265695024161222No Hit
AGAGTTCTACAGTCCACGATC144840.18258478487859134No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC139110.17536156741549874No Hit
GACTGCATAATTTGTGGTAGTGGGGG137290.17306728193856535No Hit
CCCCCCGCGGGGGCGCGCCGGC127510.16073864899108795No Hit
AGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGTG115220.1452459190397079No Hit
ACAGAGTTCTACAGTCCGACGATC114600.14446434926185145Illumina DpnII expression Sequencing Primer (95% over 24bp)
AACGATGCCGACTGGCGATGCGGCGGCGTT113810.14346847809329244No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC113280.14280036199286678No Hit
ACCTCGGCCGGCGAGCGCGCCGGCCTTCACCTTC110330.13908160256596921No Hit
CAGAGTTCTACAGTCCGACGAT105850.1334341306227485Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGAC105010.13237522963339463No Hit
TCAGAGTTCTACAGTCCGACGATC104590.13184577913871764Illumina DpnII expression Sequencing Primer (100% over 24bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTA104260.1314297823214715No Hit
CCCCCGCGGGGGCGCGCCGGC102470.12917331473701502No Hit
CTTCCCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTA100590.12680339347512776No Hit
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCC100190.12629915490876875No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTA94920.119655811796989No Hit
AGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGATC94650.11931545076469668No Hit
ATCGCATGATGAGTAATAGAAAGGAGCTT90060.11352931321572725No Hit
CGACTGCATAATTTGTGGTAGTGGGG89380.11267210765291698No Hit
TTCAGAGTTCTACAGTCCGACGATC86680.10926849732999376Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACGCGGCGCTCCCCCGGGGAGGGGGGAGGAC82550.10406223413233716No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC81080.10220915740096786No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)