FastQCFastQC Report
Tue 5 Sept 2023
SRR12764735_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764735_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37263056
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length15-44
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCGCTGGTGTAGTGGTATCATGCAAGATT9232462.477644345648945No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA8916312.392801599525278No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC7924392.126607651288719No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG7087001.9018837317046673No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT5815011.5605295496966218No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG5410531.4519823602229511No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA4888391.3118596606783943No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG3725140.9996871968847644No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC3333060.8944677001263664No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG3023120.8112914839834929No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGG2956180.7933273105673352No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA2842050.7626991194710385No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGG2812690.7548200018806831No Hit
CAGAGTTCTACAGTCCGACGATC2799210.7512024778644028Illumina DpnII expression Sequencing Primer (100% over 23bp)
CGCCGGCCGAGGTGGGATCCCGAGGCCT2759570.7405645956681599No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG2753920.7390483485841848No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGG2620200.7031629397223889No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG2577700.6917575413031073No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG2389490.6412490698562135No Hit
CGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT2298740.6168951897021007No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT2296570.6163128434769279No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG2167540.5816860538759892No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG2097140.562793346847344No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC2005340.5381576862616958No Hit
AGAGTTCTACAGTCCGACGATC1995380.5354847975968476Illumina DpnII expression Sequencing Primer (100% over 22bp)
CGCCGGCCGAGGTGGGATCCCGAGGCCTC1970760.5288777173831368No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC1881040.504800250414244No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG1824010.48949554754714697No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGG1822840.4891815636377221No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGG1794360.48153860488522465No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCT1788440.47994989997599763No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT1763720.473315983530712No Hit
CGACTGCATAATTTGTGGTAGTGGGGG1762690.47303957034549177No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG1661940.4460020670339009No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGG1645700.4416438630261565No Hit
GTTCTACAGTCCGACGATC1638170.4396230947885756Illumina DpnII expression Sequencing Primer (100% over 19bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGG1534370.4117670864139538No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG1481120.39747679310038336No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGG1433280.38463833991500856No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG1425060.38243240167956166No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC1396900.37487531886810355No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT1379710.37026217066039885No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGG1348700.3619402552490596No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1346830.3614384177186112No Hit
GACTGCATAATTTGTGGTAGTGGGGGA1224370.3285747685321354No Hit
AGTTCTACAGTCCGACGATC1214120.32582405479572046No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC1183980.31773561459908173No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTA1106200.2968623936802178No Hit
TCTACAGTCCGACGATC1103670.2961834370213758Illumina DpnII expression Sequencing Primer (100% over 17bp)
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG1054350.28294780760869426No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC1051760.2822527492109074No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGG1047170.28102096618162503No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTC1016770.2728627517828919No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGG1012750.2717839352735857No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGG990070.2656974779524256No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG976070.26194040553195636No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGG973300.26119704191733495No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT965410.25907966324608483No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTG961790.25810819166307775No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC961290.25797401050520385No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGT950060.2549603016993561No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC949350.25476976445517513No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC940840.25248600114816133No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGG935380.25102074290417836No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGT923440.24781649685414958No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTG919560.24677525106904813No Hit
CTACAGTCCGACGATC917820.24630830063964693Illumina DpnII expression Sequencing Primer (100% over 16bp)
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT905070.24288668111386247No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATG877080.2353752198960815No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCG871650.23391801252157096No Hit
GCCGCTGGTGTAGTGGTATCATGCAAGATT868350.23303241687960322No Hit
TTCTACAGTCCGACGATC853240.22897746228865393Illumina DpnII expression Sequencing Primer (100% over 18bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGG851720.2285695515687173No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC835900.22432405973358704No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGG827330.2220241946876284No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC819830.2200114773195199No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC799020.21442685752880813No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGT790590.21216456320705418No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTC787750.21140241423033046No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATG786640.21110453205985036No Hit
CCCCCCGCGGGGGCGCGCCGGC777560.20866780223286036No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG753310.20216001607597617No Hit
TCCTCGCCGCGCGGCGGCGGTGGCGGCGGGC751220.20159913883606326No Hit
GAGTTCTACAGTCCGACGATC722890.19399643443092804Illumina DpnII expression Sequencing Primer (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCC719720.1931457258900075No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGG716650.19232185358066176No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC715080.19190052474493774No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGC704440.1890451497053811No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAG692290.18578454756904533No Hit
TCCATAGTGTAGTGGTTATCACGTCTGCTTT685260.1838979604893383No Hit
CCCGGCGCCCCCCCGTCCCCGCGAGGGGGCGGGGCGGG677720.18187450862859986No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGC676650.1815873609507497No Hit
TCGACTGCAAAATTTGTGGTAGTGGGGGA662070.17767463838714675No Hit
TACAGTCCGACGATC654010.1755116381222195Illumina DpnII expression Sequencing Primer (100% over 15bp)
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCG636570.17083139933557784No Hit
CCGGCGCCCCCCCGTCCCCGCGAGGGGGCGGGGCGGG623950.1674446669108406No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGC622690.16710653039299836No Hit
TCCTCGCCGCGCGGCGGCGGTGGCGGCGGG618340.16593915431949544No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCC617080.1656010178016532No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTG614500.16490864302702385No Hit
CGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC611570.1641223414418828No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGT611160.1640123128924262No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGT594790.1596192217836347No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCG586430.1573757128239831No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG582010.15618955138837778No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGT575590.15446666532127692No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGG560940.15053515739557163No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCG555400.14904843016632882No Hit
CGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCT554520.14881227132847075No Hit
CGGGAGACCGGGGTTCAATTCCCCGACGGG545820.14647751918146487No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAG541250.14525110339849742No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGT535120.14360604240296343No Hit
GACTGCATAATTTGTGGTAGTGGGGG533420.14314982646619215No Hit
TCTTGGTCTAGGGGTATGATTCTCGCTT519110.13930956172784112No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT491860.13199668862371353No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGC488270.1310332679101789No Hit
TCGTTGGTCTAGGGGTATGATTCTCGCTT479200.12859922170634636No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG476870.12797393751065397No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCA474810.1274211111402135No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGC469920.12610881941620675No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAC465680.1249709631974361No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG453460.12169157569899797No Hit
CGTGATCGTATAGTGGTTAGTACTCTGC452800.12151445657060442No Hit
GGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT444460.11927631485726775No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCA443290.11896233094784282No Hit
AGACCGGGGTTCGATTCCCCGACGGGG440090.11810357153744985No Hit
TGGCAGGGGAGATACCATGATCACGAAGGTGGT433730.11639678720929383No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGG425560.1142042670896343No Hit
CGACAGAGTGGTTCATTTCCACCTTTCGGGCGCCA425190.11410497303280762No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGA424350.11387954868757946No Hit
GGGAGACCGGGGTTCGATTCCCCGACGG415670.11155016378688853No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTAT413940.11108589698064485No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG412920.1108121674185821No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGG410610.11019225046920468No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGG402370.1079809449874428No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCT394700.10592260602565716No Hit
CGACTGCAAAATTTGTGGTAGTGGGGGA390560.10481158603846126No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCT380630.1021467482430856No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTC380500.10211186114203838No Hit
TAAGTGGGAGGCCCCCGGCGCCCCCCCGTC375840.10086129275065363No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGG373770.10030578275705566No Hit
CCCCCCGCGGGGGCGCGCCGG372850.10005888942656768No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)