FastQCFastQC Report
Tue 5 Sept 2023
SRR12764736_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764736_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32581794
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length15-44
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG11615993.5651781482628No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA10073133.091643756632922No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT7464412.2909757516728515No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC6485531.990538028691729No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA6073931.864209809932504No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG4903691.505039900503944No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG3787541.1624712868788012No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG3544511.0878805507149176No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT3400591.0437086429310798No Hit
CGACTGCATAATTTGTGGTAGTGGGGG3207530.9844546927035387No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA2746550.8429707707316545No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC2648810.8129724225743984No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG2645940.8120915625456351No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG2348410.7207736934313684No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCG2174760.6674770578931289No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG1950070.5985152321569525No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1785400.5479747370571431No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC1590170.48805477071029296No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGG1497930.4597444818416076No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1462770.44895317918958055No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG1441950.4425631074826635No Hit
CCCCCCGCGGGGGCGCGCCGGC1433500.43996963457567745No Hit
CAGAGTTCTACAGTCCGACGATC1415230.43436220853891594Illumina DpnII expression Sequencing Primer (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATT1414150.4340307350786147No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC1401360.4301052299330111No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG1385000.4250840208491896No Hit
GACTGCATAATTTGTGGTAGTGGGGGA1313240.4030594509314005No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT1148660.35254657861994954No Hit
CGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT1145250.35149998186103565No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGG1137680.3491765984402209No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGG1113340.3417061687886186No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1112090.341322518950307No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGG1109820.3406258108439333No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG1083440.33252926465620647No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG1047090.3213727273581068No Hit
AGAGTTCTACAGTCCGACGATC1036770.3182053142930067Illumina DpnII expression Sequencing Primer (100% over 22bp)
CTCGACTGCATAATTTGTGGTAGTGGGGG1036210.31803343916544313No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG1034300.3174472222125031No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGG1031900.3167106145229449No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1014610.31140396995941966No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG990370.30396423229488223No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG940280.28859061597406205No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG938710.2881087517771428No Hit
GTTCTACAGTCCGACGATC927290.28460372685432855Illumina DpnII expression Sequencing Primer (100% over 19bp)
CGCCGGCCGAGGTGGGATCCCGAGGCCT923530.2834497081406874No Hit
GCCGCTGGTGTAGTGGTATCATGCAAGATT874320.2683461813060386No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGG873280.2680269846405634No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG800270.24561876488446277No Hit
GACTGCATAATTTGTGGTAGTGGGGG793830.24364220091748168No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGG746650.22916172142025082No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCG735550.22575491085604435No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGG726740.22305094679562457No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTC723950.2221946403565132No Hit
TCGACTGCATAATTTGTGGTAGTGGGG716170.21980680376286216No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG701660.21535339643974177No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC691490.21223202135523905No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGC687900.2111301790196083No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGA671620.20613352352543876No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTC665560.20427358910930443No Hit
AGTTCTACAGTCCGACGATC663460.20362905738094103No Hit
TCCTTAGGTCGCTGGTTCGTTTCCGGCTCGAA644760.19788965579980033No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC636460.19534222087341171No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGT610450.1873592350378251No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT606680.1862021471254775No Hit
TCGACTGCAAAATTTGTGGTAGTGGGGGA596110.1829580040927151No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGC583850.17919516647855546No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGG578310.1774948303951587No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGT573560.17603696100957486No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGG559770.1718045359933219No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGC559040.17158048448774796No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGG555110.17037428939609647No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT554550.17020241426853291No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCC548320.16829030347438817No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT545520.16743092783657032No Hit
GTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC543050.16667283575606673No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTG540540.16590246688073712No Hit
TCTACAGTCCGACGATC537430.16494794608301802Illumina DpnII expression Sequencing Primer (100% over 17bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC531700.1631892952241979No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTG513150.1574959316236546No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT512530.15730564130385208No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC510230.15659972560135885No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGT503750.15461088483955182No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCT503460.15452187807706352No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC498900.15312232346690302No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTA494450.1517565300425139No Hit
CGTGATCGTATAGTGGTTAGTACTCTGC491270.15078052485384938No Hit
CGACTGCATAATTTGTGGTAGTGGGG489530.1502464842789197No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG485650.14905563518080067No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTC482970.1482330899274607No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC474800.14572555458425646No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG472380.14498280849728531No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATG465230.14278833142214328No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG457150.14030841886729747No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGG454770.13957794957515232No Hit
TTCTACAGTCCGACGATC452600.13891193345584346Illumina DpnII expression Sequencing Primer (100% over 18bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCCG451670.13862649797613968No Hit
CCCCCCGCGGGGGCGCGCCGG431590.13246354697350307No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC423770.13006343358502606No Hit
CTACAGTCCGACGATC420330.12900762922999268Illumina DpnII expression Sequencing Primer (100% over 16bp)
CCCTGGTGGTCTAGTGGTTAGGATTCG408530.12538597475633173No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATG408390.1253430059744408No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCG408380.12533993677573432No Hit
CGACTGCAAAATTTGTGGTAGTGGGGGA398300.12224618447959006No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGC397760.12208044774943945No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGG390670.11990438586653637No Hit
GTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCG389530.11955449721399625No Hit
TTGGCAATTTTTGACAGTCTCCACGGAGACTG384610.11804445145040202No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGG381440.11707151546044396No Hit
GAGTTCTACAGTCCGACGATC381050.11695181671089075Illumina DpnII expression Sequencing Primer (100% over 21bp)
GTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTC377210.11577324440759768No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTG377110.11574255242053277No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGG375310.11519009665336415No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTA373540.114646848482315No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC373250.11455784171982672No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC372580.11435220540649174No Hit
TCCATAGTGTAGTGGTTATCACGTCTGCTTT370580.11373836566519327No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG367680.11284829804031049No Hit
TCTTGGTCTAGGGGTATGATTCTCGCTT366620.1125229629774223No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATA362140.11114796195691373No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC361240.11087173407332941No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCG356770.10949980225152733No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC351630.10792223411639028No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT350290.10751096148972031No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG347000.10650119511528432No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAC345680.10609606088602733No Hit
CGGGAGACCGGGGTTCAATTCCCCGACGGG342980.10526737723527439No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTTA341420.1047885822370616No Hit
TCGTTGGTCTAGGGGTATGATTCTCGCTT339100.10407652813715539No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGT328950.10096129145006565No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)