FastQCFastQC Report
Tue 5 Sept 2023
SRR12764737_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764737_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36353119
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length15-44
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC8804362.421899479931832No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG7962562.190337505840971No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT5156841.4185412811483933No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG4147361.1408539663405497No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA4087721.1244482213479399No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGG3915981.0772060576150289No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC3761291.0346540003898979No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG3085430.8487387285806206No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG3022620.8314609813809924No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTC3020280.8308172952092501No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG2988760.8221467874599702No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA2636910.7253600440721468No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGG2624830.7220370829804177No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT2515350.6919213726888194No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG2489500.6848105660479916No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG2438160.6706879814081427No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT2431710.668913718242443No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT2426940.6676015887385068No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT2367040.6511243230601479No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG2332130.6415212956005233No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT2187200.6016540148865906No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG2153890.5924911147238838No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT2095980.5765612573710663No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC2086780.5740305254137892No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC2040200.5612173194822706No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTT1876980.5163188336054466No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC1874920.5157521697106651No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC1867240.5136395586854597No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGG1812620.49861471308692934No Hit
CCGCCGAGGGCGCACCACCGGCCCGTC1775980.48853579798751245No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCT1749060.48113065621687096No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG1706670.46947003364415585No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG1696230.46659820303176736No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGG1658990.4563542401960063No Hit
CGACTGCATAATTTGTGGTAGTGGGGG1634120.4495130115245407No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1586170.4363229465950363No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC1574130.43301098868573007No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG1538510.42321265473809827No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGG1516200.41707562974170115No Hit
CAGAGTTCTACAGTCCGACGATC1493070.41071303950563365Illumina DpnII expression Sequencing Primer (100% over 23bp)
CCCCCCGCGGGGGCGCGCCGGC1452680.3996025760540657No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGG1430990.3936361003852242No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGG1377010.37878730570546093No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGG1371660.3773156300563921No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG1364670.3753928239279826No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCG1353300.37226516932426074No Hit
CGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT1237050.34028717040757905No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTC1227600.33768766856015847No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGG1202180.3306951461303774No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTC1177400.3238786746193635No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTG1151770.316828385481862No Hit
AGAGTTCTACAGTCCGACGATC1123740.30911790539898376Illumina DpnII expression Sequencing Primer (100% over 22bp)
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1121790.3085815002558652No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC1105350.3040591922800352No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG1083060.2979276688748495No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT1042480.2867649403067726No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGG1028290.2828615613422331No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGG1015600.27937080171855405No Hit
GTTCTACAGTCCGACGATC998910.27477972385258054Illumina DpnII expression Sequencing Primer (100% over 19bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGA987790.2717208391390021No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC984430.2707965718154748No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGG978640.2692038611597536No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGG931250.2561678407841704No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC929830.25577722780815587No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGG923950.2541597599919831No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGG898330.24711222165008728No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG897120.24677937538179323No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG878760.2417289146496618No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCG846890.23296212905418104No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT829250.22810972560566262No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCG816000.22446492142806232No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGC807220.2220497228862261No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT733390.20174059892907678No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCC704760.19386507110985443No Hit
AGTTCTACAGTCCGACGATC692910.1906053783170572No Hit
CGTGATCGTATAGTGGTTAGTACTCTGC683130.18791510021464733No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCG682070.1876235158804393No Hit
CCCCCCGCGGGGGCGCGCCGG680150.18709536312413796No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGC676940.18621235773469672No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCC672760.18506252517149904No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGG649850.17876045243875774No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG642600.1767661256245991No Hit
CCGGCGCCCCCCCGTCCCCGCGAGGGGGCGGGGCGGG635790.17489283381709284No Hit
CGGGAGACCGGGGTTCAATTCCCCGACGGG622390.17120676770540652No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC617670.1699083921794991No Hit
TAAGTGGGAGGCCCCCGGCGCCCCCCCGTCCCC616510.1695892998892337No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTC615440.16929496475941996No Hit
AGACCGGGGTTCGATTCCCCGACGGGG608660.1674299253387309No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGG602290.16567766853787705No Hit
CGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCT602150.16563915739939672No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC599180.16482217110449313No Hit
TCTACAGTCCGACGATC583640.16054743473317928Illumina DpnII expression Sequencing Primer (100% over 17bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAG571000.1570704290875289No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT566030.1557032836714781No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTA560690.15423435881801506No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG545000.14991836051261517No Hit
TCCTCGCCGCGCGGCGGCGGTGGCGGCGGGC543820.14959376663113833No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG541190.14887030738682971No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCG539270.14834215463052838No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGG538770.1482046148502416No Hit
CGGCGGCGGCGACTCTGGACGCGAGCCGGGCCC524160.14418570247026122No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC520880.1432834415115798No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC520060.14305787627190944No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT509460.1401420329298292No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGA507630.13963863733397952No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGG498700.1371821768580572No Hit
TCCTTAGGTCGCTGGTTCGTTTCCGGCTCGAA495540.1363129254466446No Hit
TAAGTGGGAGGCCCCCGGCGCCCCCCCGTC495370.1362661619213471No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC486420.13380419985421335No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATT485410.13352636949803398No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGG484330.13322928357261452No Hit
CTACAGTCCGACGATC478310.13157330461796138Illumina DpnII expression Sequencing Primer (100% over 16bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT474650.130566513426262No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGC472160.1298815653204337No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATG471500.12970001281045515No Hit
CGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC468830.1289655503837236No Hit
TTCTACAGTCCGACGATC466940.1284456500142395Illumina DpnII expression Sequencing Primer (100% over 18bp)
TCCTCGCCGCGCGGCGGCGGTGGCGGCGGG462490.12722154596968696No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTC452200.12439097729138454No Hit
GACTGCATAATTTGTGGTAGTGGGGGA446980.12295506198519031No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC446560.1228395285697494No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGC439300.12084245095998503No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTT436580.12009423455522483No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGG424010.1166364844788146No Hit
GAGTTCTACAGTCCGACGATC420870.11577273465861347Illumina DpnII expression Sequencing Primer (100% over 21bp)
TCTTGGTCTAGGGGTATGATTCTCGCTT418510.11512354689565978No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGG416580.1145926433437527No Hit
TCCTCGTTAGTATAGTGGTGAGTATCCCCG416470.11456238459208962No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGG415040.11416902082046936No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGG412120.11336578850359443No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG411660.11323925190573057No Hit
GGGAGACCGGGGTTCGATTCCCCGACGG410210.11284038654289882No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG408580.11239200685916385No Hit
TCGTTGGTCTAGGGGTATGATTCTCGCTT399580.10991629081400141No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC399220.1098172621721949No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTG398680.10966871920948516No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT393250.10817503719557048No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCT390340.10737455567430129No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGG383220.10541598920301722No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAA380720.1047282903015832No Hit
TAACCCAGAGGTCGATGGATCGTAACCATCCTCTGC380200.10458524893008493No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT378550.10413136765513847No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTG375250.10322360510524559No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGC375190.10320710033161115No Hit
TCCATAGTGTAGTGGTTATCACGTCTGCTTT372000.10232959653338136No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)