FastQCFastQC Report
Tue 5 Sept 2023
SRR12764738_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764738_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48602708
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length15-44
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTGCATAATTTGTGGTAGTGGGGGA13172412.710221414000224No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG12357602.5425743767199145No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC10943352.2515926478829122No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA8619121.7733826683072884No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG6607441.3594798051170318No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT5725841.1780907351911338No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG5487221.1289947053978966No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG4970291.022636434167413No Hit
CGACTGCATAATTTGTGGTAGTGGGGG4795260.9866240374919026No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT4609440.9483915999083837No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC4602450.9469534084397108No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG3820680.7861043462845732No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT3652450.751491048605769No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG3541820.7287289424284754No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG3521730.7245954278926187No Hit
CAGAGTTCTACAGTCCGACGATC3261620.6710778337700853Illumina DpnII expression Sequencing Primer (100% over 23bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGA3005960.6184758264909848No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCT2824430.5811260557745054No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC2738530.563452143448468No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA2729780.5616518322394711No Hit
CCCCACAACCGCGTTTGACTAGCTTGACATTT2607220.536435130322368No Hit
AGAGTTCTACAGTCCGACGATC2565370.5278244989970517Illumina DpnII expression Sequencing Primer (100% over 22bp)
CCCCACAACCGCGTTTGACTAGCTTGACATTTT2458400.5058154372797499No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTC2418200.49754429321098737No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTG2288650.47088939982521144No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC2085140.4290172473517319No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2065730.42502364271554577No Hit
GTTCTACAGTCCGACGATC2054570.42272747436212815Illumina DpnII expression Sequencing Primer (100% over 19bp)
CGCCGGCCGAGGTGGGATCCCGAGGCC2019160.4154418720866335No Hit
GCTGGTCCGATGGTAGTGGGTTATCAGAA1782660.3667820319806049No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC1738710.35773932596512936No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC1735780.357136478897431No Hit
CCCCCCGCGGGGGCGCGCCGGC1651290.3397526738633576No Hit
AGTTCTACAGTCCGACGATC1604140.33005156832002036No Hit
GACTGCATAATTTGTGGTAGTGGGGGA1597120.32860720435577373No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT1575520.3241630075427073No Hit
AGGGCACCACCAGGAGTGGAGCCTGCGG1510560.3107974971271148No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC1475280.30353864233243955No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGG1435080.295267498263677No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGG1434540.2951563933433503No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC1419570.29207631805207235No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC1411780.290473526701434No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT1398850.2878131811091678No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTC1328410.2733201615021122No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG1303030.2680982302467591No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT1261690.25959253134619575No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC1199660.24682986799830167No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1175080.241772536624914No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGG1155070.2376554820772538No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCACCC1150760.23676870021316507No Hit
TCCTCGCCGCGCGGCGGCGGTGGCGGCGGGC1142530.23507537892744576No Hit
TCCTCGCCGCGCGGCGGCGGTGGCGGCGGG1121650.23077932200814819No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGC1105960.22755110682310128No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCC1104020.22715195210933511No Hit
TCCCGGGGCTACGCCTGTCTGAGCG1085330.2233064873669179No Hit
TCGACTGCAAAATTTGTGGTAGTGGGGGA1079380.22208227574480005No Hit
TCTACAGTCCGACGATC1037050.21337288449030453Illumina DpnII expression Sequencing Primer (100% over 17bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATT1031250.21217953534605521No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1026220.2111446135881976No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCT1007050.20720038891660109No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTA993320.2043754434423695No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC989750.20364091646909882No Hit
TCGACTGCATAATTTGTGGTAGTGGGG977870.20119660822191227No Hit
GAGTTCTACAGTCCGACGATC976490.20091267342552185Illumina DpnII expression Sequencing Primer (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCC975510.20071103857011424No Hit
GACTGCATAATTTGTGGTAGTGGGGG971050.199793394228157No Hit
TTCTACAGTCCGACGATC966090.19877287495997137Illumina DpnII expression Sequencing Primer (100% over 18bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTT942000.1938163610142875No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGG935720.19252425194085893No Hit
CCGGCGCCCCCCCGTCCCCGCGAGGGGGCGGGGCGGG927750.19088442561677837No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCG920900.18947503912744945No Hit
ACAAGCGGTGGAGCATGTGGTTTAATTTG918860.1890553094284376No Hit
CTGTCCCCGCCGGCGGGCCGCCTCG887620.18262768403768778No Hit
TAAGTGGGAGGCCCCCGGCGCCCCCCCGTC875550.18014428331853444No Hit
CTCCTCGCCGCGCGGCGGCGGTGGCGGCGGG866560.17829459214494797No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTA841150.17306648839402117No Hit
CGGCGGCGGCGACTCTGGACGCGAGCCGGG803350.16528914397115485No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGG802790.16517392405377906No Hit
CGACTGCATAATTTGTGGTAGTGGGG797800.1641472322900197No Hit
CGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT791710.16289421568855794No Hit
CTCCTCGCCGCGCGGCGGCGGTGGCGGCGGGC773490.1591454533767954No Hit
TAAGTGGGAGGCCCCCGGCGCCCCCCCG764270.15724843973714386No Hit
CCCCCCGCGGGGGCGCGCCGG757440.15584316824486405No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT753720.15507777879372484No Hit
CGACTGCAAAATTTGTGGTAGTGGGGGA753000.15492963889995595No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGT750360.15438645928947003No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG747280.15375274974390316No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCC738120.15186808109539904No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC725460.1492632879632962No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGG721450.14843823105494452No Hit
CTGGTCCGATGGTAGTGGGTTATCAGAA713950.14689510716151866No Hit
CGGGAGACCGGGGTTCAATTCCCCGACGGG713800.14686424468365014No Hit
CCGCCGAGGGCGCACCACCGGCCCGTC678980.13970003482110502No Hit
CTACAGTCCGACGATC676450.13917948769438937Illumina DpnII expression Sequencing Primer (100% over 16bp)
GCCGGCCGAGGTGGGATCCCGAGGCCTC668680.1375808113408002No Hit
CTTTAAAATCTGTGTGGCTGTCACTCGGCT643680.13243706502938069No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG641960.132083175283155No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCC637130.13108940349578874No Hit
CGCGCCGCGGCTGGACGAGGCGCCGCCG621480.1278694183048401No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGG612550.12603207212240108No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTG610890.1256905273673228No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGG608370.12517203773913174No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCT596930.12281825942702616No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTT580000.11933491442493287No Hit
CAAGCGGTGGAGCATGTGGTTTAATTTG576820.1186806298941203No Hit
CTGTCCCCGCCGGCGGGCCGCCTCGC576150.11854277749297425No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAC570360.1173514858472495No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGG567410.116744523782502No Hit
TCGACTGCAAAATTTGTGGTAGTGGGGG558270.11486397013104702No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC552200.11361506852663435No Hit
CTCCCGGGGCTACGCCTGTCTGAGCG545510.1122386020136985No Hit
GTTGGTCCGAGTGTTGTGGGTTATTGTTA544980.11212955459189639No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG544850.11210280711107704No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCG534750.11002473360126354No Hit
GGGCGCGCGCCGCGGCTGGACGAGGCGCCG533820.10983338623847873No Hit
GGGAGACCGGGGTTCGATTCCCCGACGG528040.10864415209127853No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATG526710.10837050478751102No Hit
GCCGGCCGAGGTGGGATCCCGAGGCC502450.10337901336690952No Hit
CGGCGCCCCCCCGTCCCCGCGAGGGGGCGGGGCGGG501560.10319589599822297No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG493020.10143879225824207No Hit
TAAGTGGGAGGCCCCCGGCGCCCCCCCGTCC491950.10121863991611332No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)