FastQCFastQC Report
Tue 5 Sept 2023
SRR12764739_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764739_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33969369
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length15-44
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG10151032.9882892437595765No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC7770312.2874460812033335No Hit
GCCGGGCGCGGTGGCGCGT5340791.5722370350771013No Hit
ATCGCATGATGAGTAATAGAAAGGAGCTT5321391.5665260075923104No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA4486651.320792859001885No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA4261181.2544183555484942No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG3407971.0032479555331157No Hit
CGACTGCATAATTTGTGGTAGTGGGGG3348890.9858558161619075No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG3308780.9740481196456725No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG3125520.9200995167146024No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT2519380.741662289929495No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1788460.5264919698685012No Hit
ATCTTGAGTTGTGATGCATAGTGA1653470.4867532275915988No Hit
GCATAATCTTGAGTTGTGATGCATAGTGAAG1581910.4656871901270818No Hit
TAATCAAACTCCGAATACCATTAA1446460.4258130317345606No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG1392440.40991046963515865No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT1325590.3902309754414337No Hit
ACAAGCGGTGGAGCATGTGGTTTAATTTG1243900.3661828396047039No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC1189900.3502861651625027No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1141290.3359762143359213No Hit
GACTGCATAATTTGTGGTAGTGGGGGA1129520.3325113280732415No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC1115230.32830459700325904No Hit
GGAGTGGACGTACCGCTGGTGTTCCAGTT1112720.3275656960245567No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG1097860.323191166724351No Hit
AGCGGGAAGCTTCCTCAAAGATGAGTATTCCT1070160.3150367615012219No Hit
GACTGCATAATTTGTGGTAGTGGGGG1043450.3071737953095331No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTC1004340.2956604816533389No Hit
TCGACTGCATAATTTGTGGTAGTGGGG982160.2891310698176348No Hit
GCTGGTCCGATGGTAGTGGGTTATCAGAA973460.2865699389352802No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC967630.28485368686124257No Hit
CGACTGCATAATTTGTGGTAGTGGGG957060.2817420600306117No Hit
CAAGCGGTGGAGCATGTGGTTTAATTTG928040.27319906943222877No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTC903360.2659337004464228No Hit
AGCTTCACTTAAAAATGGGGGTGCGGAACA856610.25217130173951713No Hit
ATCTTGAGTTGTGATGCATAGTGAAG850730.2504403305224775No Hit
CCCCACAACCGCGTTTGACTAGCTTGACATTTT827630.24364008645553586No Hit
CCCCACAACCGCGTTTGACTAGCTTGACATTT779590.22949793385917766No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCT762510.22446987460968143No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG760960.2240135811766183No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA742640.2186204871806715No Hit
TATCAACCTGTACCGAGAACGAACACACGTTCCCAA740610.21802288997478875No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT717510.21122264590784715No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTA715800.21071925121717744No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG682140.20081032414820543No Hit
ATCTTGAGTTGTGATGCATAGTG676870.1992589264757906No Hit
GTTGGTCCGAGTGTTGTGGGTTATTGTTA663640.19536424123745133No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC655040.19283254864110075No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG653240.19230265949302738No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTA645790.1901095071857237No Hit
TATCAACCTGTACCGAGAACGAACACACGTTCCCA595720.17536975738348276No Hit
AAGCGGGAAGCTTCCTCAAAGATGAGTATTCCT585890.17247597386928207No Hit
TGGAAACAATAGCTAATACCGGATATAGTTATTT583400.17174296054778057No Hit
CTGGTCCGATGGTAGTGGGTTATCAGAA575260.16934668406704878No Hit
GGAAGATTAGCTCAGTTGGGAGAGCATCG574890.16923776240883368No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA560770.16508107642505812No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG556730.16389176967049343No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCT547790.16125998690172905No Hit
CCCGCGAGGGGGCGGGGCGGGGTCCGCCGG541510.15941126254067306No Hit
TAGTTCAACCTTGAGCTTAC539750.1588931487070013No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT537920.15835442807312672No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT535200.15755370669381583No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCG534900.1574653918358036No Hit
CGGAGTGGACGTACCGCTGGTGTTCCAGTT531970.15660285005588417No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT510060.15015292159239108No Hit
AAGGCGAGCGAGAAAACCAATGTTAAGGAACT498880.14686172121713537No Hit
GGAGTGGACGTACCGCTGGTGTTCCAGT494470.1455634928043556No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC494180.14547812177494376No Hit
AGTTCAACCTTGAGCTTAC489970.14423876993417217No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG477060.14043828721104593No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTC476100.1401556796654068No Hit
TTAGTTCAACCTTGAGCTTAC473600.1394197225153049No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCT468510.13792131375769742No Hit
TTGGCAATTTTTGACAGTCTCCACGGAGACTG463120.1363345901420777No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCG455920.13421503354978423No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT448520.1320366003854826No Hit
CGGGCGGGTCGCGCCGTCGGGCCCGGGGG434810.12800061137432373No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC431220.12694377690677738No Hit
GACTGCATAATTTGTGGTAGTGGGG427050.12571620038040743No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG422880.12448862385403744No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG419610.12352599190170416No Hit
CGCCGGGCGCGGTGGCGCGT417120.12279297858020265No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT414800.12211001034490808No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTC413960.12186272874247384No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTT409070.12042319655687453No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGG405640.1194134633469347No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCC404950.11921033917350657No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG403110.11866867471103158No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTT398850.11741460372725794No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG388800.11445605598384827No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG385300.11342571597370561No Hit
CGACTGCAAAATTTGTGGTAGTGGGGGA373900.11006975136924092No Hit
TAGTAATACATTCAGCTGACCAATACTAATATA364440.10728488951325531No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTC357190.10515061377795978No Hit
TAATCAAACTCCGAATACCATTA353530.1040731725102106No Hit
AAGGCGAGCGAGAAAACCAATGTTAAGGAACTC350960.10331660855990583No Hit
TAAGCGGGAAGCTTCCTCAAAGATGAGTATTCCT345730.10177698620189264No Hit
TCGACTGCAAAATTTGTGGTAGTGGGGGA342910.1009468265365777No Hit

[OK]Adapter Content

Adapter graph