FastQCFastQC Report
Tue 5 Sept 2023
SRR12764740_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764740_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357375
Total Bases9 Mbp
Sequences flagged as poor quality0
Sequence length15-44
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCT269207.532703742567332No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGC209745.868905211612452No Hit
CCCCGCGCAGGTTCGTATCCTGC182265.099965022735222No Hit
AAATTCTAGAATTAAAGCATCTATGC157214.3990206365862194No Hit
ATGATGAACCGACGA148944.16761105281567No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC97302.7226302903112978No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCT75532.113466246939489No Hit
CCCCGCGCAGGTTCGAATCCTGC70741.9794333683105982No Hit
CAAATTCTAGAATTAAAGCATCTATGC70131.9623644630989856No Hit
CCCCGCGCAGGTTCGGATCCTGC54371.5213711087792934No Hit
TGCTGGCATACTAATTGTTA34170.9561385099685205No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAA33930.9494228751311647No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC32290.9035327037425673No Hit
GTGGGGGGCCGGGCCACCCCTCCCACGGCG30900.8646379853095487No Hit
CAGAGTTCTACAGTC28230.7899265477439665Illumina DpnII expression Adapter 1 (100% over 15bp)
CGTGGGGGGCCGGGCCACCCCTCCCACGGCG27950.782091640433718No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGC27790.7776145505421477No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT21580.6038474991255683No Hit
TCGACTGCATAATTTGT21560.6032878628891221No Hit
TTGCTGGCATACTAATTGTTA20650.577824414130815No Hit
TATGATGAACCGACGA20410.5711087792934593No Hit
CGACTGCATAATTTGT19110.5347324239244491No Hit
GGGCGCGCGCCGCGGCTGGACGAGGCGC19020.5322140608604407No Hit
ACCATGGTAGGCACGG17930.5017138859741168No Hit
TCGCTTCTGGCGCCAAGCGCCCGGC16410.4591815320041973No Hit
TGGCATACTAATTGTTA16070.44966771598461003No Hit
CCCTGGTGGTCTAGTGGTTAGGATT16030.44854844351171735No Hit
CGGCGGCGGCGACTCTGGACGCGAGC15530.43455753760055965No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAA14790.4138509968520462No Hit
CTGGCATACTAATTGTTA14370.40209863588667366No Hit
CCAGGCGGCCCGGGTT13310.37243791535501924No Hit
CCTGGTGGTCTAGTGGTTAGGATT12520.35033228401539No Hit
CCCGCGCAGGTTCGTATCCTGC12350.34557537600559635No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGAC12260.34305701294158797No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCT11940.334102833158447No Hit
TAGAATTAAAGCATCTATGC11870.3321441063308849No Hit
TAAACCAATTTTTTGAGGCCTTG11680.326827562084645No Hit
TTAAACCAATTTTTTGAGGCCTTG11590.3243091990206366No Hit
CGCCGCGGCTGGACGAGGCGCCGC11250.3147953830010493No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCT11010.3080797481636936No Hit
TCCTCGTTAGTATAGTGGTGAGTATCCC10150.2840153899965023No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC9890.2767401189227003No Hit
TTGCGACCCGGGTTCGTTTCCCGGGCGG9720.2719832109129066No Hit
TAAGTCAGAATCCCGCCCAGGCGGAACGATA9540.2669464847848898No Hit
CTGTCCCCGCCGGCGGGCCGCCT9320.26079048618398043No Hit
ACCCGTGGTCACCATGGTAGGCACGG9110.25491430570129414No Hit
TTTGCTGGCATACTAATTGTTA8970.25099685204617No Hit
TGGGGGGCCGGGCCACCCCTCCCACGGCG8810.2465197621545995No Hit
CTTTGCTGGCATACTAATTGTTA8790.24596012591815322No Hit
TTTAAACCAATTTTTTGAGGCCTTG8730.24428121720881427No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGG8660.24232249038125217No Hit
CGCCGAGGGCGCACCACCGGCCCGTCT8430.23588667366211963No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGC8040.22497376705141658No Hit
TTATGATGAACCGACGA7570.21182231549492828No Hit
CACCATGGTAGGCACGG7380.20650577124868835No Hit
TTCTTTGCTGGCATACTAATTGTTA7230.20230849947534105No Hit
AAACCAATTTTTTGAGGCCTTG7170.2006295907660021No Hit
AGCCTCTTCTCGTTCCTCATCA7050.19727177334732424No Hit
TTCTAGAATTAAAGCATCTATGC7000.19587268275620845No Hit
AATTCTAGAATTAAAGCATCTATGC6720.18803777544596012No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGC6610.18495977614550543No Hit
TGGGCGCGCGCCGCGGCTGGACGAGG6460.1807625043721581No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC6300.17628541448058763No Hit
AGCCCCTCTCCGGCCCCGGCCGGGGGGCGGG6150.1720881427072403No Hit
GGCTCGTTGGTCTAGGGGTATGATTCT6070.16984959776145506No Hit
AGAATTAAAGCATCTATGC5990.16761105281566982No Hit
CCCAGGCGGCCCGGGTT5900.16509268975166141No Hit
TTTTAAACCAATTTTTTGAGGCCTTG5820.16285414480587618No Hit
GGCGCCGGCGCGCCCCGCTCGCTCCGCCGT5790.1620146904512067No Hit
CTAGAATTAAAGCATCTATGC5770.16145505421476042No Hit
TTGGTGGTTCAGTGGTAGAATTCT5730.16033578174186777No Hit
ACACTCGTCCACTATTCGAAACC5620.15725778244141309No Hit
CGCGCCGCGGCTGGACGAGGCGCCGC5410.15138160195872682No Hit
TCCTTGGTATAGTGGTGAGTATCCC5360.14998251136761107No Hit
CCATGGTAGGCACGG5340.14942287513116476No Hit
AAGCCTCTTCTCGTTCCTCATCA5290.14802378454004897No Hit
CCCGCGCAGGTTCGAATCCTGC5240.14662469394893318No Hit
TTTATGATGAACCGACGA5220.1460650577124869No Hit
GGGCGCGCGCCGCGGCTGGACGAGGCGCCGC5200.14550542147604056No Hit
TGCGAGAGGTCCCGGGTTCATATCCCGGA5170.14466596712137111No Hit
GGTCACCATGGTAGGCACGG5140.14382651276670164No Hit
GCTGGCATACTAATTGTTA4910.13739069604756907No Hit
CCGCCATGACGACTTGAAA4910.13739069604756907No Hit
CTCGACTGCATAATTTGT4890.13683105981112276No Hit
CTCTTCTCGTTCCTCATCA4840.135431969220007No Hit
CACTGCACTCCAGCCTGGGCAACATAG4780.13375306051066807No Hit
GACTGCATAATTTGT4780.13375306051066807No Hit
TCCCCGCGCAGGTTCGTATCCTGC4760.13319342427422176No Hit
AGTCAGAATCCCGCCCAGGCGGAACGATA4620.12927597061909757No Hit
CGCTTCTGGCGCCAAGCGCCCGGC4610.12899615250087443No Hit
ACTCCGGATGTGCTGACCCCTG4540.12703742567331233No Hit
ATTCTAGAATTAAAGCATCTATGC4540.12703742567331233No Hit
AGATGAGGCAGAAATG4530.1267576075550892No Hit
CCTCACTTCATCCGG4400.12311997201818817No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATA4360.12200069954529556No Hit
CCCCGCGCAGGTTCGATCCTGC4210.11780342777194823No Hit
TCTTTGCTGGCATACTAATTGTTA4160.11640433718083246No Hit
TCTAGAATTAAAGCATCTATGC4150.1161245190626093No Hit
TAAGTCAGAATCCCGCCCAGGCGGAA4100.11472542847149353No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG3840.10745015739769151No Hit
CAGGCGGCCCGGGTT3820.1068905211612452No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGG3820.1068905211612452No Hit
ATCTTGAGTTGTGATGCATAGTGAAGACTTCGGT3730.1043721580972368No Hit
CTAAGTCAGAATCCCGCCCAGGCGGAACGATA3730.1043721580972368No Hit
CTTGTTACGACTTTTACTTCCTCTA3710.10381252186079047No Hit
TTTCTTTGCTGGCATACTAATTGTTA3700.10353270374256734No Hit
TGCGAGAGGTCCCGGGTTCAGATCCCGGA3680.10297306750612102No Hit
CTAAGTCAGAATCCCGCCCAGGCGGAA3600.10073452256033578No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)