FastQCFastQC Report
Tue 5 Sept 2023
SRR12764753_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764753_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14980667
Total Bases501.3 Mbp
Sequences flagged as poor quality0
Sequence length15-50
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG3445092.2996906613036656No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT1794581.1979306395369447No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGG1455050.9712851904391173No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1278740.8535935015443572No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT887050.5921298430837558No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGA746300.49817541501990537No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC731650.488396144177025No Hit
CAGAGTTCTACAGTCCGACGATC730650.48772861715703314Illumina DpnII expression Sequencing Primer (100% over 23bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGA719150.480052056427127No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG666050.44460637166556066No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCT655930.43785099822324336No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCC482780.32226869471165737No Hit
TAACCGAAAGGTTGGTGGTTCGTGCCCACCCAGGGACGC475240.31723554098091894No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC450770.3009011548017188No Hit
TAATCTCAGGGTCGTGGGTTCGTGCCCCACGTTGGGCGCCA439950.29367851244540716No Hit
GGTTCCATAGTGTAGTGGTTATCACGTCTGCTTT439920.29365848663480737No Hit
AGAGTTCTACAGTCCGACGATC408810.27289172104286147Illumina DpnII expression Sequencing Primer (100% over 22bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGG408170.27246450375006664No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCCTT396770.2648546957221598No Hit
CAAGAATGTAGTTTA390940.2609630131956074No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC365700.2441146312110135No Hit
CGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCC360470.2406234648964562No Hit
TAATCTCAGGGTCGTGGGTTCGGGCCCCACGTTGGGCGCCA360010.24031640246725997No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGG352960.23561033697631753No Hit
GTTCTACAGTCCGACGATC343310.22916870123339633Illumina DpnII expression Sequencing Primer (100% over 19bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG327930.21890213566592198No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG309400.20653285998547327No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG305580.20398290676910447No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG299140.19968403276035707No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGG297690.1987161185813689No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGC292990.19557874158740732No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCG292510.19525832861781123No Hit
ACCGTTTGACAGGTGTACCGCCC289880.19350273255523268No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGC287870.19216100324504912No Hit
GCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT283900.18951092097568153No Hit
CGCCCGGGGCACCCCCCTCGCGGC278250.18573939331272765No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGG271320.18111343106418426No Hit
AGTTCTACAGTCCGACGATC261480.17454496518746462No Hit
TAACCGAAAGGTTGGTGGTTCGGGCCCACCCAGGGACGC259820.17343687033427818No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTC257040.17158114521870088No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCG238910.15947888034624894No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCC238750.15937207602305023No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGG235640.15729606699087564No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGG225460.1505006419273588No Hit
TCTACAGTCCGACGATC225140.1502870332809614Illumina DpnII expression Sequencing Primer (100% over 17bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGC222960.14883182437737918No Hit
TAATCCCAGGGTCGTGGGTTCGTGCCCCACGTTGGGCGCCA216440.14447954820703243No Hit
GGTAGCGTGGCCGAGCGGTCTAAGGCCTGGATT214820.14339815443464568No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCAC214540.14321124686904796No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG214340.1430777414650496No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAC212320.1417293368846661No Hit
GGTAGCGTGGCCGAGCGGTCTAAGGCTCTGGATT208710.13931956434249557No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT207760.13868541367350334No Hit
CCCCCCGCGGGGGCGCGCCGGC205950.13747718976731813No Hit
CTAGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGA204790.13670285842412758No Hit
TCCTCGTTAGTATAGTGGTGAGTATCCCCGCCTGT204670.13662275518172853No Hit
GCATTGGTGGTTCAGTGGTAGAATTCT204320.13638912072473142No Hit
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCAC203350.13574161951533933No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGG200400.13377241480636343No Hit
GGTTCCATAGTGTAGCGGTTATCACGTCTGCTTT200080.13355880615996604No Hit
GGGGATGTAGCTCAGTGGTAGAGCATGCTT198680.13262426833197746No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGA198620.13258421671077797No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC198290.13236393279418066No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGG198010.13217702522858296No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCG196840.13139601861519248No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG192520.12851230188882778No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC187290.12502113557427047No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCT186970.1248075269278731No Hit
GGCTCGTTGGTCTAGGGGTATGATTCTCGCTT178930.11944060968713877No Hit
TGGGAAACTCGACTGCATAATTTGTGGTAGTGGGGGA178630.11924035158114121No Hit
CGCAGAAGGTCCTGGGTTCGAGCCCCAGTGGAACCACCA175860.11739130173576383No Hit
AGGGGGTCCCCCCCGCGGGGGCGCGCCGGC175610.11722441998076587No Hit
TTCTACAGTCCGACGATC175400.1170842393065676Illumina DpnII expression Sequencing Primer (100% over 18bp)
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG170940.11410706879740401No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC170890.11407369244640442No Hit
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGG169930.11343286650721225No Hit
CTGTCCCCGCCGGCGGGCCGCCTCGC165050.11017533464965212No Hit
AGCAGTTGACCCGGGTTCGTTTCCCGGCCAACG164930.1100952314072531No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC164720.10995505073305481No Hit
TAATCTCAGGGTCGTGGGTTCGTGCCCCACGTTGGGCGC160410.10707800927689001No Hit
TAACCGAAAGGTTGGTGGTTCGTGCCCACCCAGGGACG160180.1069244780622919No Hit
GGTAGCGTGGCCGAGCGGTCTAAGGCTGGATT160070.10685105009009278No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAG160020.10681767373909318No Hit
CTAGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGA159190.10626362631249997No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGG158970.10611677036810177No Hit
GAGTTCTACAGTCCGACGATC158670.10591651226210422Illumina DpnII expression Sequencing Primer (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGGA157200.10493524754271621No Hit
CTACAGTCCGACGATC155640.10389390539152896Illumina DpnII expression Sequencing Primer (100% over 16bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC153490.10245872229854651No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC153200.1022651394627489No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGC151930.10141738014735925No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC151840.10135730271555998No Hit
CCGCCGCGGCCCGGGTTCGATTCCCGGTCAGG151760.10130390055396064No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAG150460.10043611542797125No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)