FastQCFastQC Report
Tue 5 Sept 2023
SRR12764754_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764754_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27948924
Total Bases928.4 Mbp
Sequences flagged as poor quality0
Sequence length15-50
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT4268391.5272108507647735No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2876551.0292167240499135No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCT1866060.6676679216702582No Hit
CAGAGTTCTACAGTCCGACGATC1443250.5163883947732657Illumina DpnII expression Sequencing Primer (100% over 23bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGG1426030.510227155793189No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCC1372270.4909920682456327No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1215650.4349541327601735No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC1199140.4290469286044787No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGA1037040.3710482736294249No Hit
GTTCTACAGTCCGACGATC1010810.36166329694839056Illumina DpnII expression Sequencing Primer (100% over 19bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCG990330.3543356445493215No Hit
AGAGTTCTACAGTCCGACGATC988380.3536379432710898Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGC967310.34609919151091467No Hit
GGTTCCATAGTGTAGTGGTTATCACGTCTGCTTT964900.34523690429012577No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGG926720.33157627105787685No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT920030.32918261898025125No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC919510.32899656530605614No Hit
TAATCTCAGGGTCGTGGGTTCGTGCCCCACGTTGGGCGCCA889790.3183628822347508No Hit
TGTGAGCTTTATCACTACCAA888460.31788701418344406No Hit
AATTATTCTTTTCTTGGCACTGA887340.31748628319287No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG799910.28620422024118No Hit
TAACCGAAAGGTTGGTGGTTCGTGCCCACCCAGGGACGC792680.28361735857881326No Hit
TCTACAGTCCGACGATC778210.2784400572988069Illumina DpnII expression Sequencing Primer (100% over 17bp)
TAATCTCAGGGTCGTGGGTTCGGGCCCCACGTTGGGCGCCA758770.27148451224812803No Hit
AGTTCTACAGTCCGACGATC739940.26474722246910115No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG737900.2640173195934126No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGG708130.25336574674574236No Hit
GCATTGGTGGTTCAGTGGTAGAATTCT697390.24952302278255864No Hit
ATTATTCTTTTCTTGGCACTGA686020.24545488763717702No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC628830.22499256143098745No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCG627960.22468127932223797No Hit
AAATTATTCTTTTCTTGGCACTGA626390.22411954034437961No Hit
GGTAGCGTGGCCGAGCGGTCTAAGGCTCTGGATT625050.22364009433779988No Hit
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCAC617270.22085644513541916No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG613900.2196506742084239No Hit
TGAGCTTTATCACTACCAA603070.21577574864778334No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGG599050.21433741062804423No Hit
TTCTACAGTCCGACGATC588120.21042670551467382Illumina DpnII expression Sequencing Primer (100% over 18bp)
GGTAGCGTGGCCGAGCGGTCTAAGGCCTGGATT582040.20825130870870018No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGC578480.20697755663151826No Hit
CTACAGTCCGACGATC566260.20260529528793308Illumina DpnII expression Sequencing Primer (100% over 16bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCG558530.19983953586191727No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGG540570.19341352819163987No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG522590.1869803646108165No Hit
TTATTCTTTTCTTGGCACTGA519870.18600716077656515No Hit
TCCTCGTTAGTATAGTGGTGAGTATCCCCGCCTGT516210.18469762914665339No Hit
GGGGATGTAGCTCAGTGGTAGAGCATGCTT516100.18465827163865056No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTT507580.18160985374606908No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCCTT506130.18109105023148656No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGG504810.1806187601354528No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAC492420.17618567355222692No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGG485280.17363101348731708No Hit
TTGTGAGCTTTATCACTACCAA485030.1735415646054925No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT480280.1718420358508256No Hit
TAATCCCAGGGTCGTGGGTTCGTGCCCCACGTTGGGCGCCA477370.17080085086638755No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT472560.16907985438008277No Hit
GGCTCGTTGGTCTAGGGGTATGATTCTCGCTT467870.16740179335705374No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC461240.16502960901106603No Hit
GGTAGCGTGGCCGAGCGGTCTAAGGCTGGATT439390.15721177673959827No Hit
GTCAGGATGGCCGAGCGGTCTAAGGCTGCGTT422410.1511364086860732No Hit
TAACCGAAAGGTTGGTGGTTCGGGCCCACCCAGGGACGC413310.1478804693876587No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGC409790.14662102913156871No Hit
GAGTTCTACAGTCCGACGATC409620.146560203891928Illumina DpnII expression Sequencing Primer (100% over 21bp)
TAACCCAGAGGTCGATGGATCGTAACCATCCTCTG408540.14617378472244585No Hit
CGCAGAAGGTCCTGGGTTCGAGCCCCAGTGGAACCACCA407060.14564424734204437No Hit
GACCCAGTGGCCTAATGGATAAGGCATCAGCCT399520.14294646906621522No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAG391220.13997676618963936No Hit
CCGCCGCGGCCCGGGTTCGATTCCCGGTCAGG390660.13977640069435232No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTT389880.13949732018305963No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCAC383660.13727183200326423No Hit
CGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCC383280.13713586970289088No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGA377660.13512505883947445No Hit
TACAGTCCGACGATC367220.13138967353448025Illumina DpnII expression Sequencing Primer (100% over 15bp)
CGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATG364530.13042720356604784No Hit
AGCAGTTGACCCGGGTTCGTTTCCCGGCCAACG363550.1300765639492955No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA360170.12886721506702728No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAC348130.12455935691835579No Hit
TAACCGAAAGGTTGGTGGTTCGTGCCCACCCAGGGACG346610.12401550771686237No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGC339120.12133561921739815No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC335800.1201477380667678No Hit
TCCTTGGTATAGTGGTGAGTATCCCCGCCTGT334550.11970049365764492No Hit
AGCAGTTGACCCGGGTTCGTTTCCCGGCCAACGCACCA331230.11851261250701457No Hit
GGTAGCGTGGCCGAGCGGTCTAAGGCGCTGGATT330340.11819417448771909No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTC319240.114222644134708No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAC317890.11373962017285531No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGACT315910.1130311850288047No Hit
TATTCTTTTCTTGGCACTGA315860.11301329525243978No Hit
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG314290.11245155627458145No Hit
CTAGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGA314150.11240146490075968No Hit
ATTCTTTTCTTGGCACTGA310110.11095597097047456No Hit
GCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT309130.11060533135372223No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGG308960.11054450611408154No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGG308950.11054092815880855No Hit
CGCAGAAGGTCCTGGGTTCGAGCCCCAGTGGAACCAC305900.10944965180054873No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGG302800.10834048566592404No Hit
GGTTCCATAGTGTAGTGGTTATCACGTCTGCTT301400.1078395719277064No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCG300740.10760342687968953No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGG299620.10720269588911545No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT291970.10446556010528349No Hit
AAACGCTTCGGGCCCCGCG290350.10388593135106025No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG283210.10133127128615041No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCC282230.10098063166939808No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)