FastQCFastQC Report
Tue 5 Sept 2023
SRR12764755_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764755_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43526992
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length15-50
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG8088281.8582216754146483No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT6274971.441627301054941No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGG4130820.9490249176878568No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG3870600.8892413240960919No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCT2812310.6461071327878573No Hit
CAGAGTTCTACAGTCCGACGATC2751110.6320468917309976Illumina DpnII expression Sequencing Primer (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC2199640.5053507947436386No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG2121300.4873527672208546No Hit
AGAGTTCTACAGTCCGACGATC1996230.4586188726296547Illumina DpnII expression Sequencing Primer (100% over 22bp)
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT1864270.4283020522070535No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGA1706220.39199124993521256No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCC1699490.3904450829039599No Hit
GTTCTACAGTCCGACGATC1666320.3828245241481424Illumina DpnII expression Sequencing Primer (100% over 19bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC1604360.3685896787905767No Hit
TAATCTCAGGGTCGTGGGTTCGTGCCCCACGTTGGGCGCCA1583590.3638179270462797No Hit
GGTTCCATAGTGTAGTGGTTATCACGTCTGCTTT1534090.3524456732502903No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG1413590.3247617018883363No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG1321970.30371269395321415No Hit
TAACCGAAAGGTTGGTGGTTCGTGCCCACCCAGGGACGC1317010.30257317114860593No Hit
AGTTCTACAGTCCGACGATC1305920.3000253268133024No Hit
TAATCTCAGGGTCGTGGGTTCGGGCCCCACGTTGGGCGCCA1302460.2992304177600878No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG1183630.2719301163746854No Hit
TCTACAGTCCGACGATC1127860.25911737709787064Illumina DpnII expression Sequencing Primer (100% over 17bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGA1099140.2525191724711875No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGC1072470.246391939971409No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGC1062340.24406464843699746No Hit
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCAC1058580.2432008166335041No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1019490.2342201822721864No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCG984740.2262366303649009No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGG974180.22381054955508986No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGC969990.22284792847619703No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGG939570.21585916159793445No Hit
TTCTACAGTCCGACGATC926190.21278520693550337Illumina DpnII expression Sequencing Primer (100% over 18bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCCTT916760.21061873515174218No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGG888960.2042318936259138No Hit
GCATTGGTGGTTCAGTGGTAGAATTCT863990.1984952233777147No Hit
TAATCCCAGGGTCGTGGGTTCGTGCCCCACGTTGGGCGCCA834150.19163970714999098No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGG827640.19014408346894265No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC807480.18551247465021245No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC787700.18096816798183527No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCG785430.18044665250472627No Hit
TCCTCGTTAGTATAGTGGTGAGTATCCCCGCCTGT776050.1782916678460115No Hit
GAGTTCTACAGTCCGACGATC764740.17569328016050362Illumina DpnII expression Sequencing Primer (100% over 21bp)
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC755170.17349464442661236No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCG744730.17109613271691276No Hit
CTACAGTCCGACGATC725050.1665748002986285Illumina DpnII expression Sequencing Primer (100% over 16bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT706360.162280912956264No Hit
TAACCGAAAGGTTGGTGGTTCGGGCCCACCCAGGGACGC700940.1610357086012284No Hit
CGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCC683480.15702440453500668No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGG672890.15459143145016777No Hit
CGCAGAAGGTCCTGGGTTCGAGCCCCAGTGGAACCACCA659130.15143017463738362No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG658790.15135206218706773No Hit
GCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT649420.14919937495336227No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGG646500.14852852685064935No Hit
GGCTCGTTGGTCTAGGGGTATGATTCTCGCTT634040.14566593528907304No Hit
GGTAGCGTGGCCGAGCGGTCTAAGGCCTGGATT617780.14193032222396623No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCG589260.1353780660974689No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTT581850.13367567416558443No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAC571690.13134149035614498No Hit
GGTTCCATAGTGTAGCGGTTATCACGTCTGCTTT557480.12807684941794276No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGC554620.12741978586528563No Hit
GGTAGCGTGGCCGAGCGGTCTAAGGCTCTGGATT554470.12738532449014625No Hit
TAACCGAAAGGTTGGTGGTTCGTGCCCACCCAGGGACG554020.12728194036472817No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGGG550610.12649851843656No Hit
GGGGATGTAGCTCAGTGGTAGAGCATGCTT549850.1263239141358539No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT549730.12629634503574244No Hit
CCGCCGCGGCCCGGGTTCGATTCCCGGTCAGG545160.12524642180649653No Hit
CTAGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGA539100.12385418225086631No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGG534600.12282034099668546No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAC530940.12197948344328502No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAG530880.12196569889322928No Hit
CGCGGGCCGGTCCCCCCCGCCGGGT516800.11873092448014787No Hit
AGCAGTTGACCCGGGTTCGTTTCCCGGCCAACGCACCA514560.11821630127806673No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC502750.11550304234209431No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGA499840.11483449166439068No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCAC495610.11386268088546067No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT482170.11077494167297387No Hit
TAATCTCAGGGTCGTGGGTTCGTGCCCCACGTTGGGCGC477960.10980772574406245No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTC477350.10966758281849572No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTT470790.10816047201240096No Hit
GGTAGCGTGGCCGAGCGGTCTAAGGCTGGATT467020.10729434278389832No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGG464980.106825668082003No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA458830.10541275170128916No Hit
GACCCAGTGGCCTAATGGATAAGGCATCAGCCT457810.10517841435034152No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGC452910.10405267609578901No Hit
CTCCTCGCCGCGCGGCGGCGGTGGCGGCGGGC443660.10192755796219505No Hit
CGCAGAAGGTCCTGGGTTCGAGCCCCAGTGGAACCAC441550.1014428012852347No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC440220.101137243758999No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)