FastQCFastQC Report
Tue 5 Sept 2023
SRR12764756_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764756_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37963432
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length15-50
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG9221322.429000623547418No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG3749440.9876451633772204No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT3722770.9806199818815117No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGG3694630.9732075856576929No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT2432120.6406480847147855No Hit
CAGAGTTCTACAGTCCGACGATC2420270.6375266598657361Illumina DpnII expression Sequencing Primer (100% over 23bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCT1898800.5001655277109825No Hit
AGAGTTCTACAGTCCGACGATC1775170.467599978842798Illumina DpnII expression Sequencing Primer (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC1727830.45513008412938005No Hit
GTTCTACAGTCCGACGATC1447230.38121685099492586Illumina DpnII expression Sequencing Primer (100% over 19bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGG1215580.32019760489515275No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG1145460.30172719895292927No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG1128530.29726764429517333No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGC1126980.29685935665669005No Hit
AGTTCTACAGTCCGACGATC1097540.2891045256393047No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG995280.2621680779545959No Hit
TCTACAGTCCGACGATC981330.2584934892082465Illumina DpnII expression Sequencing Primer (100% over 17bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGA973000.25629927241562356No Hit
TAATCTCAGGGTCGTGGGTTCGTGCCCCACGTTGGGCGCCA942960.2483863945704382No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC929040.24471970816547883No Hit
TAACCGAAAGGTTGGTGGTTCGTGCCCACCCAGGGACGC918220.24186959703748598No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC879660.231712454237541No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG867640.22854624945394822No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGG857530.22588316040551867No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCG840750.22146311745471275No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGG824970.21730648588357346No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG800120.21076071309885785No Hit
TAATCTCAGGGTCGTGGGTTCGGGCCCCACGTTGGGCGCCA769160.20260549678437922No Hit
GCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT756560.19928651340057982No Hit
TTCTACAGTCCGACGATC755800.19908632075203314Illumina DpnII expression Sequencing Primer (100% over 18bp)
GCATTGGTGGTTCAGTGGTAGAATTCT755060.19889139633107986No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGA734680.1935230724134741No Hit
CTGTCCCCGCCGGCGGGCCGCCTCGC705210.18576033905469874No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCG676670.17824257827901333No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGG670230.17654620899396029No Hit
GAGTTCTACAGTCCGACGATC667650.1758666076344204Illumina DpnII expression Sequencing Primer (100% over 21bp)
CTACAGTCCGACGATC659610.17374878014190076Illumina DpnII expression Sequencing Primer (100% over 16bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGG621400.16368383132483913No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT619240.16311486274475923No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGG586800.154569797588374No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCC552380.14550317790024886No Hit
AGGGGGTCCCCCCCGCGGGGGCGCGCCGGC528940.13932881516086323No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG509880.1343081942644174No Hit
CGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCC509680.1342555119884841No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGC507780.1337550303671175No Hit
GGTTCCATAGTGTAGTGGTTATCACGTCTGCTTT506180.13333357215965091No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCT503410.13260392263797435No Hit
CCCCGGGGAGCCCGGCGGGCGCCGGCGCGCCCCGCTCGCTCCGCCGTCG493050.12987498074462814No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT492600.12975644562377817No Hit
TAACCGAAAGGTTGGTGGTTCGGGCCCACCCAGGGACGC492050.12961156936496152No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGG486150.12805744222492846No Hit
GGCTCGTTGGTCTAGGGGTATGATTCTCGCTT476770.12558664348365556No Hit
GGTAGCGTGGCCGAGCGGTCTAAGGCTCTGGATT450300.11861414426388003No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC446210.11753679172104355No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTC444640.11712323585496696No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC441940.11641202512986708No Hit
TAATCCCAGGGTCGTGGGTTCGTGCCCCACGTTGGGCGCCA439370.11573505788412386No Hit
GGTAGCGTGGCCGAGCGGTCTAAGGCCTGGATT439240.11570081440476719No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC437470.11523457626275728No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC436990.11510813880051729No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG434200.11437322105124742No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC429900.11324055211868093No Hit
TCCTCGTTAGTATAGTGGTGAGTATCCCCGCCTGT419090.11039307510448476No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTT418500.11023766239048145No Hit
GGGGATGTAGCTCAGTGGTAGAGCATGCTT406150.10698453185159866No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC406150.10698453185159866No Hit
CGCAGAAGGTCCTGGGTTCGAGCCCCAGTGGAACCACCA403790.10636288099558544No Hit
CTCCTCGCCGCGCGGCGGCGGTGGCGGCGGGC394150.10382359529559919No Hit
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCAC392310.10333891835701262No Hit
TACAGTCCGACGATC390400.10283580262184935Illumina DpnII expression Sequencing Primer (100% over 15bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCCTT387900.1021772741726828No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGG386170.10172157248585956No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)