FastQCFastQC Report
Tue 5 Sept 2023
SRR12764757_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764757_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24746845
Total Bases794 Mbp
Sequences flagged as poor quality0
Sequence length15-50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG3619561.4626349338673275No Hit
AGCCACTGCACTCCAGCCTGGGCAA3341541.350289299504644No Hit
TAGCCACTGCACTCCAGCCTGGGCAA2225100.8991449213020893No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1954380.7897491579229594No Hit
CAGAGTTCTACAGTCCGACGATC1837980.7427128589523231Illumina DpnII expression Sequencing Primer (100% over 23bp)
CACTGCACTCCAGCCTGGGCAA1820510.735653373187572No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT1435020.579879980660161No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT1427030.5766512862548742No Hit
AGAGTTCTACAGTCCGACGATC1388030.5608917015482175Illumina DpnII expression Sequencing Primer (100% over 22bp)
GTTCTACAGTCCGACGATC1330000.5374422476885438Illumina DpnII expression Sequencing Primer (100% over 19bp)
CCACTGCACTCCAGCCTGGGCAA1039750.42015456919861904No Hit
ATAGCCACTGCACTCCAGCCTGGGCAA984720.3979173910856111No Hit
AGTTCTACAGTCCGACGATC976490.39459171462059106No Hit
TCTACAGTCCGACGATC921880.37252425511211634Illumina DpnII expression Sequencing Primer (100% over 17bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGG826670.3340506638320966No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGG778820.3147148656727757No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC737930.2981915472457196No Hit
TTCTACAGTCCGACGATC727520.293984950404789Illumina DpnII expression Sequencing Primer (100% over 18bp)
CTACAGTCCGACGATC625480.25275141134152657Illumina DpnII expression Sequencing Primer (100% over 16bp)
ACTGCACTCCAGCCTGGGCAA621960.25132900779877193No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC611350.24704159257473024No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGC604050.2440917215911766No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGG570680.23060717436909633No Hit
GAGTTCTACAGTCCGACGATC560020.2262995545492769Illumina DpnII expression Sequencing Primer (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCG551610.22290114153945684No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT543220.2195108103679479No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGG540120.2182581254297265No Hit
AAACCATCCAATCGGT530800.21449198877675113No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCG525180.21222099221133037No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG518440.2094974127004877No Hit
AGGGGGTCCCCCCCGCGGGGGCGCGCCGGC499860.20198938490947027No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG484390.19573808297582984No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG480890.1943237612713863No Hit
CTGTCCCCGCCGGCGGGCCGCCTCGC474740.19183859599072123No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCT439140.1774529237969527No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG426820.17247451139731146No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGG422240.1706237704240682No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG409250.1653746164410049No Hit
GCATTGGTGGTTCAGTGGTAGAATTC404050.16327333848011738No Hit
GCCACTGCACTCCAGCCTGGGCAA382730.15465809884047846No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGG377120.15239114319421324No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT371440.15009590111385918No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGG365020.14750163101599414No Hit
TACAGTCCGACGATC361310.146002450009284Illumina DpnII expression Sequencing Primer (100% over 15bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG357190.1443375913171962No Hit
CAGAGTTCTACAGTCCGACGA355130.1435051619711523Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGC322080.13014992416204976No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC321720.13000445107244984No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT321350.1298549370636944No Hit
CTGCACTCCAGCCTGGGCAA316490.12789105035409565No Hit
AATAGCCACTGCACTCCAGCCTGGGCAA301600.1218741217314773No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG300770.1215387254415664No Hit
GTTTCCGTAGTGTAGTGGTTATCACGT296840.11995064421343407No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT295730.11950210218716768No Hit
AATTATTCTTTTCTTGGCACTGATAACAC288370.11652798568868071No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC283580.11459238541317086No Hit
GCATTGGTGGTTCAGTGGTAGAATTCT275090.1111616450501064No Hit
ACTAAACCATCCAATCGGT274420.11089090346668433No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC274160.11078583956863997No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGG271650.10977156886059618No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGG270910.10947254084308525No Hit
TAACCGAAAGGTTGGTGGTTCGTGCCCACCCAGGGACGC270200.10918563558304098No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTC267200.1079733598363751No Hit
AGAGTTCTACAGTCCGACGA264610.10692676177508689No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA259380.10481336105673268No Hit
GGCTCGTTGGTCTAGGGGTATGATTC259290.10477699278433272No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGG258130.10430824616228856No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC257130.10390415424673327No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC257070.10387990873179996No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCG255980.10343944854384467No Hit
ACTCCAGCCTGGGCAA250040.10103914256544622No Hit
CGCCCGGGGCACCCCCCTCGCGG248500.10041684101549106No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)