FastQCFastQC Report
Tue 5 Sept 2023
SRR12764759_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764759_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23882901
Total Bases719.1 Mbp
Sequences flagged as poor quality0
Sequence length15-44
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT5658612.3693143475325718No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA5207802.1805558713323814No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT5127202.1468078773177512No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC5083452.1284893321795373No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG2972831.2447524695597072No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA2706591.133275224814607No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC2184900.9148386119424938No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC2105410.8815553855873707No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT2003870.8390396124825875No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT1988620.83265429103441No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA1860720.7791013327903508No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG1770770.7414384039861824No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGG1729230.72404520707095No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTC1677270.7022890560907991No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG1641260.687211323281037No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG1627580.6814833759098193No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT1561930.6539950904624191No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG1544550.6467179175595126No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG1534320.6424345183191942No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCT1461740.6120445753219008No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC1370530.573854072417752No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG1257060.5263430937472797No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT1225860.5132793541287133No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT1220890.5111983674010122No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGG1195680.5006426983053692No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC1193760.4998387758673036No Hit
CAGAGTTCTACAGTCCGACGATC1166660.48849174562168973Illumina DpnII expression Sequencing Primer (100% over 23bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG1156320.4841622883250238No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC1113650.4662959495582216No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG1063520.4453060371518518No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG1037730.43450751648637664No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTC1020240.4271842855271225No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG1014000.42457153760340927No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGG985450.41261737843321467No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT965780.40438136053907353No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG964970.40404220576051464No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGG957590.40095212888919984No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGG948150.39699951023537716No Hit
CGACTGCATAATTTGTGGTAGTGGGGG938660.39302595610139657No Hit
TCCATAGTGTAGTGGTTATCACGTCTGCTTT925060.3873315054984317No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT897420.375758372067112No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC895060.3747702174036563No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT829800.34744522870148814No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTT825620.3456950225602827No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGG802720.33610657264793753No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGG789930.3307512768235316No Hit
GACTGCATAATTTGTGGTAGTGGGGGA779550.3264050711427393No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGT729440.3054235329284328No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCG721410.30206129481506455No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG669430.28029676964285033No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTC655410.27442646100655865No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG654360.2739868159232415No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC649110.27178859050665577No Hit
AGAGTTCTACAGTCCGACGATC632200.264708211117234Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGA626360.2622629470347844No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGG612100.2562921480937345No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG601760.25196269079706857No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT592020.24788445926229816No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG587970.24618868536950347No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT587810.24612169183299804No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGA572310.2396316929840307No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCG567890.23778099653806714No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGT560230.2345736809778678No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGG556340.2329449006215786No Hit
GTTCTACAGTCCGACGATC556140.23286115870094676Illumina DpnII expression Sequencing Primer (100% over 19bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC542930.22733000484321397No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC522200.21865015476972416No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC516430.2162342003594957No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT515490.21584061333252605No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG509230.2132194912167496No Hit
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGG496770.20800236956138618No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGG490270.20528075714085153No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGT489330.20488717011388188No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGG474130.19852278414586233No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGG470820.19713685535940545No Hit
CGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT462310.19357363663652083No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC462180.19351920438811013No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGC456450.19111999836200802No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCC451080.18887152779304323No Hit
TCCTTAGGTCGCTGGTTCGTTTCCGGCTCGAA449940.18839419884544176No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCT447830.18751072158277587No Hit
TCCTCGCCGCGCGGCGGCGGTGGCGGCGGGC446230.1868407862177212No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGG440790.18456300597653527No Hit
TCTTGGTCTAGGGGTATGATTCTCGCTT433270.18141430976077821No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG431040.18048058734573325No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGG428080.1792412069203821No Hit
GCCGCTGGTGTAGTGGTATCATGCAAGATT421730.1765824009403213No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGG418560.17525509149830668No Hit
AGACCGGGGTTCGATTCCCCGACGGGG405170.16964856991200525No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGC403690.16902887969932964No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGC400780.16781043475413646No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATG399790.16739591224700887No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG399700.16735822838272452No Hit
TCCCTGGTGGTCTAGTGGTTAGGAT395390.16555358999310846No Hit
GACTGCATAATTTGTGGTAGTGGGGG394410.16514325458201246No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT393980.164963209452654No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGG392760.16445238373679982No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG391920.1641006676701461No Hit
TCCTCGCCGCGCGGCGGCGGTGGCGGCGGG391590.16396249350110356No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGA386450.16181032614086538No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAG384870.16114876496787386No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGCCT375420.15719195921801962No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTA367240.15376691466417752No Hit
TCGTTGGTCTAGGGGTATGATTCTCGCTT366240.15334820506101834No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGT365880.15319746960388103No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAC361110.15120022479681175No Hit
AGTTCTACAGTCCGACGATC358390.15006133467621877No Hit
CCCCCCGCGGGGGCGCGCCGGC354960.14862516073738277No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC349920.14651486433746053No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCC345470.14465160660340215No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCC343660.14389374222168405No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC342690.14348759390661964No Hit
GTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG342230.14329498748916641No Hit
GTGGGGGGCCGGGCCACCCCTCCCACGGCGC341930.14316937460821866No Hit
TCGACTGCAAAATTTGTGGTAGTGGGGGA331180.13866824637425748No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG327950.13731581435605333No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT317280.13284818289034486No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGT315020.1319018991872051No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTG312760.13095561548406537No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGG302250.12655497755486236No Hit
TCCGTAGTGTAGTGGTTATCACGTTCG301360.1261823260080507No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTG298950.12517323586443707No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGC294880.12346908777957921No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGC291360.12199522997645888No Hit
CGTGATCGTATAGTGGTTAGTACTCTGC287810.12050881088524379No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGT286850.12010684966621099No Hit
GTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC284860.11927361755592421No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTT282190.1181556629154892No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTC280060.11726381146076016No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATG279330.11695815345045395No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCG279270.1169330308742644No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGT277590.11622959874095698No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCA275390.11530843761400679No Hit
AGCTACCATCTGTGGGAT269260.11274174774664099No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCG269040.11264963163394597No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGC267840.11214718011015497No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGC266330.11151492860938461No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC263050.11014156111102248No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGC260470.1090612903348718No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCG259550.10867607749996534No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACG257120.10765861316428854No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTG255020.10677932299765426No Hit
ATCCTTAGGTCGCTGGTTCGTTTCCGGCTCGAA253740.10624337470561052No Hit
GCGCCGCTGGTGTAGTGGTATCATGCA252760.10583303929451451No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCG250680.10496212331994341No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGG246140.10306118172160074No Hit
TCTACAGTCCGACGATC244090.10220282703512441Illumina DpnII expression Sequencing Primer (100% over 17bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATT244060.10219026574702963No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCC239970.1004777434701086No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)