FastQCFastQC Report
Tue 5 Sept 2023
SRR12764760_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764760_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19952834
Total Bases601.7 Mbp
Sequences flagged as poor quality0
Sequence length15-44
%GC70

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC12689196.35959282776572No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG10092475.058163667376775No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT5974112.994116023818972No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC3073231.5402473653617326No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA2753701.3801047009161707No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT2590521.2983218323772954No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCT2105171.0550731790782202No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA2038281.0215491192880168No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT1974570.9896188180586277No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1871970.938197551285196No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG1777190.8906955272619418No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTC1743840.8739811096508896No Hit
CAGAGTTCTACAGTCCGACGATC1698410.8512124142364939Illumina DpnII expression Sequencing Primer (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATT1663770.8338514719262436No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1590170.7969644813363356No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG1465860.7346625547027555No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGG1404890.7041054919817405No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG1382430.6928489456685701No Hit
AGAGTTCTACAGTCCGACGATC1310620.6568590707465415Illumina DpnII expression Sequencing Primer (100% over 22bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGG1301450.652263232380924No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT1251090.6270237100153292No Hit
GTTCTACAGTCCGACGATC1210000.6064301442090884Illumina DpnII expression Sequencing Primer (100% over 19bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG1130610.566641310201849No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG1125600.564130388695661No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG1005380.5038782961858952No Hit
CGACTGCATAATTTGTGGTAGTGGGGG998890.5006256254124101No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG991210.49677654813346317No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGG974840.4885721998188327No Hit
AGTTCTACAGTCCGACGATC919700.46093702779264345No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGG908310.4552285655260802No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGG869850.4359531082151037No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCG857920.4299740077023645No Hit
CCCCCCGCGGGGGCGCGCCGGC833390.4176800147788529No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC798550.4002188360811301No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTC783270.39256077607822526No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT782220.3920345350439942No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG781880.39186413318529084No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCT777230.3895336371765535No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA761440.38161997438559353No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG720000.36085099490127565No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC712940.3573126504234937No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGG707710.3546914688910858No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC700790.35122328988453466No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG686830.34422679003894885No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGG651520.32653005583066547No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGG630790.3161405542691329No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTC615650.3085526597374588No Hit
TCTACAGTCCGACGATC615270.30836221060126096Illumina DpnII expression Sequencing Primer (100% over 17bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGG610970.3062071282706006No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGG596760.299085332940674No Hit
TTCTACAGTCCGACGATC590650.29602311130338677Illumina DpnII expression Sequencing Primer (100% over 18bp)
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG582910.29214396310819807No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG579490.29042992088241704No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC574630.2879941766668334No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT565260.28329810191374316No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC556990.2791533272917522No Hit
GACTGCATAATTTGTGGTAGTGGGGGA541460.27136997180450656No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGG534680.26797195826918624No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG522240.26173725496839195No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGC522180.26170718405215015No Hit
GAGTTCTACAGTCCGACGATC518110.25966737356708325Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGCTTCACTTAAAAATGGGGGTGCG517640.25943181805652266No Hit
CGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT510860.25603380452120234No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC507700.25445006959913563No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCC470410.23576099515487375No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG461950.23152099596478376No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC461700.23139570048044306No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAG442800.22192336186428455No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC431940.21648052602452364No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGG430490.21575381221534745No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG418950.2099701726581798No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGG415700.2083413313617504No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCC408160.20456241955403429No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCG397990.19946539925105375No Hit
CCCCCCGCGGGGGCGCGCCGG392210.19656856765309627No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG390220.19557121559774415No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTC388590.19475428903984265No Hit
CTACAGTCCGACGATC384770.19283977403911645Illumina DpnII expression Sequencing Primer (100% over 16bp)
GCCGGCCGAGGTGGGATCCCGAGGCCTCT382050.1914765591694894No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATG379170.1900331551898843No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG357340.17909235349725258No Hit
GACTGCATAATTTGTGGTAGTGGGGG351740.17628573464802041No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG349780.17530341805078917No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTG348880.17485235430716256No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGT348070.17444639693789865No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTA335430.1681114572496318No Hit
AGACCGGGGTTCGATTCCCCGACGGGG329310.16504422379297096No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGT327440.16410701357010238No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTC326060.1634153824965416No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGC321440.16109992194592507No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC316260.15850379951038535No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCG315430.15808781850237416No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGG314390.15756658928751674No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGG314250.15749642381628595No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCG312500.15661935542590089No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG304780.15275023086945944No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCA294940.14781860060580868No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCC294180.14743770233341288No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGC290640.14566351827514828No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG274880.13776489094230926No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATG272630.13663723158324276No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCG266580.13360508086219733No Hit
GTGGGGGGCCGGGCCACCCCTCCCACGGCGC262700.13166049494522933No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTT258050.12932999893649194No Hit
GTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGC248600.12459382962841269No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC244490.12253397186585124No Hit
TCCTCGCCGCGCGGCGGCGGTGGCGGCGGG241710.12114068607998242No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGG241580.12107553242812524No Hit
TAAGTGGGAGGCCCCCGGCGCCCCCCCGTC239610.12008820401152036No Hit
CGGGAGACCGGGGTTCAATTCCCCGACGGG239580.12007316855339949No Hit
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGG238830.11969728210037732No Hit
TCCTCGCCGCGCGGCGGCGGTGGCGGCGGGC236270.11841425634072833No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGG234570.11756224704721145No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC234350.1174519870209916No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCC232440.11649472952062849No Hit
TCGACTGCAAAATTTGTGGTAGTGGGGGA232280.11641454041065044No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGA231120.11583316936330948No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCT230300.11542220017467192No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG228930.11473558092048478No Hit
CGGCGGGACCACCCCCC224710.1126205931448134No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGC224650.11259052222857165No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGT223950.11223969487241761No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC223450.11198910390373619No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGT220320.11042040443979037No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT215400.10795458930796499No Hit
GTGGGGGGCCGGGCCACCCCTCCCACGGCG211690.10609520432034868No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGG210480.10548877417613958No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTG210170.10533340777555711No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGT209460.10497756860002945No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGT209260.10487733221255686No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGC208790.10464177670199631No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC207280.10388499197657836No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGG205000.102742297159391No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGGG204570.10252678892632494No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGG203160.10182012239464329No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG201260.10086787671365381No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCG200290.10038173023441183No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)