FastQCFastQC Report
Tue 5 Sept 2023
SRR12764761_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764761_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21574214
Total Bases656.2 Mbp
Sequences flagged as poor quality0
Sequence length15-44
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC5854602.713702571041522No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG4022801.8646333998541036No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT3435601.5924566243757476No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT2947901.3663997214452401No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT2481101.1500303093313156No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT2444591.1331073289622509No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA2336141.0828389854666316No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG2289641.0612854771905016No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2220451.0292147839082342No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT2113500.9796417148731352No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTC2092050.9696992900876946No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCT2061340.9554647042993084No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT1903490.8822986552372198No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTT1892800.8773436659152448No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT1854460.8595724507043455No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA1645320.7626326502555318No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC1589080.7365644931490899No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG1432740.6640983537105918No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT1377240.6383731986713398No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1333130.6179274943689721No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG1327040.6151046800592596No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT1323220.6133340477664678No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAA1214850.5631027855754096No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG1110820.5148831841567901No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCC1103690.5115783128877835No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTC1093790.5069895014483494No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC1092900.5065769719351074No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG1015040.4704875922710325No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT951610.4410867529171631No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT928160.43021729551769533No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG914120.42370952656722516No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG897350.4159363580986079No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT871910.40414450324818324No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC813250.3769546366787685No Hit
CAGAGTTCTACAGTCCGACGATC804960.37311208649362615Illumina DpnII expression Sequencing Primer (100% over 23bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC794990.3684908289127011No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG781620.3622936158879299No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT739430.3427378628950283No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCC727740.3373193572660399No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG717170.3324199899009067No Hit
CGACTGCATAATTTGTGGTAGTGGGGG710490.3293237009700562No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC689740.3197057375995251No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTC679910.31514937230158185No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC672990.3119418394570481No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC668020.3096381634111908No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTT609760.28263370336458143No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA601050.2785964763304934No Hit
GTTCTACAGTCCGACGATC586120.27167617786678117Illumina DpnII expression Sequencing Primer (100% over 19bp)
AGAGTTCTACAGTCCGACGATC580390.2690202294275935Illumina DpnII expression Sequencing Primer (100% over 22bp)
CCCCCCGCGGGGGCGCGCCGGC572690.2654511538635892No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGG561990.26049152937854425No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGA559070.25913806176206466No Hit
ATTGATCATCGACACTTCGAACGCA535680.24829641534101776No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG524700.243207006290009No Hit
AGCTTCACTTAAAAATGGGGGTGCGGAACA512850.23771433805189843No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC503350.23331093313526974No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCG497790.2307337824682744No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAT491810.22796195495233337No Hit
GACTGCATAATTTGTGGTAGTGGGGGA483080.2239154575921051No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTC452080.2095464520746851No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG421440.19534431242778996No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC412960.19141369414431506No Hit
AGTTCTACAGTCCGACGATC394610.18290816991061645No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG394430.182824736975354No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTG388390.18002509848099219No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG385760.17880605059354654No Hit
CTTCCCACGCAGTTTTATCCGGTAAAGCG379900.17608984503444713No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG364840.1691092894508231No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTA361870.16773264601899285No Hit
CATTGATCATCGACACTTCGAACGCA361530.16757505047460824No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG345730.16025149282379417No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCT327650.15187111799298922No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGC323370.14988726819897125No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCG321840.1491780882492405No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGG320130.14838547536424734No Hit
CGCCGGCCGAGGTGGGATCCCGAGGC320060.1483530292227564No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATA319090.14790341840495325No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCC315480.14623012453663434No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGG313490.14530772708567738No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC309270.14335168827008019No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTTA308920.14318945756262547No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCT308420.14295769940911868No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGC304510.1411453506486957No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATT302550.14023685868694916No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCG297780.1380258859024945No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT296470.1374186795403068No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG292680.13566195273672543No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCC290580.13468856849199698No Hit
TCTACAGTCCGACGATC288910.13391449625928437Illumina DpnII expression Sequencing Primer (100% over 17bp)
GCCGGCCGAGGTGGGATCCCGAGGCCTCT284080.1316757124964089No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGG278070.1288899794912575No Hit
TTCTACAGTCCGACGATC274600.12728157790592046Illumina DpnII expression Sequencing Primer (100% over 18bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGC273880.12694784616487073No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG273510.1267763451312757No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGG272820.12645651887943635No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCC272240.1261876794213685No Hit
TCCTCGCCGCGCGGCGGCGGTGGCGGCGGGC271980.12606716518154495No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG271750.12596055643093185No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGG271650.1259142048002305No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG264580.1226371445096447No Hit
TCCTCGCCGCGCGGCGGCGGTGGCGGCGGG259430.12025003552852494No Hit
GACTGCATAATTTGTGGTAGTGGGGG258880.11999510155966749No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCG253780.11763116839389838No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG238350.11047911177667932No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGG236410.10957989014107304No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC231610.107355011867408No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCC231020.10708153724627001No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCGTTA227030.10523210718128596No Hit
GAGTTCTACAGTCCGACGATC226160.10482884799418418Illumina DpnII expression Sequencing Primer (100% over 21bp)
TTCCCACGCAGTTTTATCCGGTAAAGCG223740.10370713853121138No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCC223610.10364688141129963No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGA220280.1021033721089445No Hit
GTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC220050.1019967633583314No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCG216130.10017977943483827No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)