FastQCFastQC Report
Tue 5 Sept 2023
SRR12764762_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764762_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50510944
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length15-44
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT12355362.446075844474417No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT10190042.017392508047365No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCC8410541.6650926183442543No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC8216431.6266633227048775No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTT8184701.6203815157364707No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT6369891.2610910617706927No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCC6210341.2295038477206048No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG6143341.2162393955654442No Hit
ATTGATCATCGACACTTCGAACGCA5298761.0490320671892412No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT5259311.0412218785695235No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT5148411.019266240599265No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT4897700.9696314525422451No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC4667110.9239799596697302No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG4494420.8897913291820482No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT3887680.7696708261876872No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG3725010.737465924216344No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA3699450.7324056347075992No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG3501300.6931765124009561No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT3386780.6705041980605233No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT3317400.6567685608885077No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG3316630.6566161186771722No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG3267990.6469865223663213No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTC3104140.6145480076555291No Hit
CATTGATCATCGACACTTCGAACGCA3070630.6079138018089704No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG3003540.5946315317330042No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT2928060.5796882354841755No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT2868660.5679284077525852No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG2641850.5230252675538988No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA2639740.5226075362994601No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG2637270.5221185333617997No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT2624170.5195250360001191No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCT2551510.5051400346031941No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCC2400670.4752771993332771No Hit
ATTGATCATCGACACTTCGAACGCAC2215520.4386217766985309No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGG2018230.39956291452402876No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG1976020.39120630966627745No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTC1963720.3887711938228674No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC1947610.3855817859986937No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAT1883010.37279247839834473No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAA1871590.3705315822250323No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCC1854930.3672332871070475No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC1838590.36399834459637104No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC1838310.36394291106497634No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCC1798420.3560456126102098No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTT1798200.35600205769268534No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG1747600.34598442666207146No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1720330.3405855966580233No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG1643710.3254166067456589No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTG1609880.31871904829179193No Hit
CCGCCGAGGGCGCACCACCGGCCCGTC1540990.30508041979971706No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCC1429780.28306340899112875No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT1425870.28228931932058127No Hit
GAGCCTCGGTTGGCCTCGGATAGCCGGTCCCC1393840.27594811928282315No Hit
CGACTGCATAATTTGTGGTAGTGGGGG1382220.273647627729943No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATAC1372580.2717391304347826No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC1356770.2686091156799604No Hit
CTTCCCACGCAGTTTTATCCGGTAAAGCG1327620.2628380891079763No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1297150.25680573303084575No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTC1296060.2565899382122021No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATA1265540.2505476832901797No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCG1167640.231165744991818No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG1141040.2258995595093214No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGG1128990.22351393789037086No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGG1095040.2167926222087633No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC1083490.2145059890387319No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1003000.19857082853173363No Hit
CGCTTCTGGCGCCAAGCGCCCGGCCG997280.19743840067609902No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGG981460.19430640615229838No Hit
GGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCC980580.19413218648220076No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCC962940.1906398740043346No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT960950.19024589997763652No Hit
TTCCCACGCAGTTTTATCCGGTAAAGCG941340.18636357301102907No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCG901730.17852170808765722No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGA869290.17209933752178538No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTC835580.165425536295659No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG831740.16466530500796026No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGG813360.1610264896256938No Hit
GGACCGGGGTCCGGTGCGGAGTGCCCTTCG811310.16062063698512544No Hit
GACCGGGGTCCGGTGCGGAGTGCCCTTCG793540.1571025875105403No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCC767360.15191955232513574No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC766540.15175721126890837No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG745760.14764325133183018No Hit
CATTGATCATCGACACTTCGAACGCAC737370.14598222515896753No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGG733650.14524575109900936No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATT718880.14232163231793885No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG699470.13847890073089902No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGG696160.13782359719905454No Hit
ACATTGATCATCGACACTTCGAACGCA695960.13778400181948688No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGC694440.1374830769347728No Hit
CGTGATCGTATAGTGGTTAGTACTCTGC691250.13685153063066885No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC691030.13680797571314446No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG667490.1321475995380328No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGC666980.1320466313201353No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCC659790.13062317742467852No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGG627280.124186948475958No Hit
GACTGCATAATTTGTGGTAGTGGGGGA619980.12274171712173901No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC617370.12222499741838125No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGG608920.1205520926316483No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCT604200.11961764167385192No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCG600820.11894847975915873No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG598760.11854064734961199No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG585560.11592735229814752No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGG571080.11306064681745011No Hit
AAGGGCAGGGCGCCCTGGAATGGGTT566920.11223706292244309No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT560610.11098782869708393No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTTA560160.11089873909305675No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGG547880.10846758278760342No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGC536800.10627399875955594No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT532830.10548803047513822No Hit
CAGAGTTCTACAGTCCGACGATC532410.10540488017804617Illumina DpnII expression Sequencing Primer (100% over 23bp)
TCTCGCTTCTGGCGCCAAGCGCCCGGCCG532110.10534548710869471No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC531420.10520888304918632No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG530750.10507623852763473No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG527490.10443083384068214No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCG527330.10439915753702803No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG519050.10275990882292756No Hit
TTGGAACAATGTAGGTAAGGGA508350.10064155601605862No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGG506340.1002436224514038No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)