FastQCFastQC Report
Tue 5 Sept 2023
SRR12764763_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764763_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38403494
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length15-44
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT17504414.558025371337306No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC14552943.789483321491529No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG9563972.49039058789807No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT8553462.2272608841268453No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC4920151.2811724891490341No Hit
CTCCCGGGGCTACGCCTGTCTGAGCG4902351.276537494218625No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT4487501.1685134691129928No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG3936301.0249848620544786No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTA3881001.010585130613376No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTT3757780.9784995084041052No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC3474650.9047744457835007No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT3467730.9029725264060608No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG3321240.8648275597006877No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCC3293290.8575495760880507No Hit
TCCCGGGGCTACGCCTGTCTGAGCG3223480.8393715425997437No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCC3005860.7827048236808869No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT2875980.748884984267317No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTT2855690.7436016108325977No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAA2434550.633939713922905No Hit
CGACTGCATAATTTGTGGTAGTGGGGG2293260.597148790680348No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC2290740.5964926003868294No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCC2278020.5931804017624022No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA2166800.5642194952365532No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2130980.5548922189215387No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTTA2042980.5319776372431113No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT2008970.5231216722103462No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA1939110.5049306190733582No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAT1874570.48812485655602067No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT1834070.47757894112447163No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG1739080.45284421256045093No Hit
AACGATGCCGACTGGCGATGCGGCGGCG1669810.4348067912778978No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC1665930.433796466540258No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC1644150.42812510757484723No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA1585050.4127358828339942No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT1560370.4063093842450898No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTT1544130.40208060235352544No Hit
ATTGATCATCGACACTTCGAACGCA1512750.3939094708413771No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCC1397590.3639226159994713No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCG1382710.3600479685520281No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCGTTA1349730.3514602082820901No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG1318580.3433489671538741No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGA1291470.33628971363907667No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTT1283700.33426646023406104No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG1267310.32999861939645386No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCGTT1228190.31981204626849835No Hit
ATTGATCATCGACACTTCGAACGCAC1197170.3117346562268527No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCC1196650.3115992518805711No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC1166600.3037744430233353No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC1138440.2964417768862385No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1137770.2962673135939141No Hit
CAGAGTTCTACAGTCCGACGATC1036080.26978795210664946Illumina DpnII expression Sequencing Primer (100% over 23bp)
AACGATGCCGACTGGCGATGCGGCGGCGT1033440.26910051465629664No Hit
CGACTGCATAATTTGTGGTAGTGGGG1029480.2680693584807674No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG914860.23822311584461558No Hit
TGAAGGGCAGGGCGCCCTGGAATGGGTT913420.23784814996260498No Hit
CCGCCGAGGGCGCACCACCGGCCCGTC886710.23089305363725499No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG880030.22915362857348345No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCC876020.22810945274927327No Hit
AAACGATGCCGACTGGCGATGCGGCGGCG858920.22365673290039703No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCC858090.22344060673229366No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT841600.21914672659732473No Hit
CCCGGGGCTACGCCTGTCTGAGCG825700.21500647831679065No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCC824630.21472785783501888No Hit
GTTCTACAGTCCGACGATC823430.21441538626667667Illumina DpnII expression Sequencing Primer (100% over 19bp)
AGAGTTCTACAGTCCGACGATC822300.21412114220648779Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGCGGCGTCCGGTGAGCTCTCGCTGGCC819820.21347536763191394No Hit
TCGACTGCATAATTTGTGGTAGTGGGG787520.20506467458403654No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCC785040.20441890000946267No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCC770360.2005963311567432No Hit
CCCCCGCGGGGGCGCGCCGGC753570.19622433312968868No Hit
CCCCCCGCGGGGGCGCGCCGGC740350.19278193801845217No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGG734020.19113365049544712No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATA713890.18589193993650682No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA704520.18345205777370152No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA703750.18325155518401529No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCC657120.1711094308241849No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCG649010.16899764380813892No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATAC643610.16759152175059905No Hit
GAAGGGCAGGGCGCCCTGGAATGGGTT635200.1654016168424675No Hit
ACGATGCCGACTGGCGATGCGGCGGCGTTA599800.15618370557637282No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT578940.150751908146691No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGG576660.15015821216684086No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTC576160.1500280156800316No Hit
AGTTCTACAGTCCGACGATC573050.14921819353207808No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG554520.1443931117309274No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC551530.1436145367398081No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG551030.14348434025299886No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC542800.14134130608011863No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG526980.13722188923747405No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCC514770.13404249102959226No Hit
CATTGATCATCGACACTTCGAACGCA490950.1278399303979997No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGC486670.12672544847091258No Hit
GACTGCATAATTTGTGGTAGTGGGGG482700.12569168836564715No Hit
CGTGATCGTATAGTGGTTAGTACTCTGC478580.12461886931433895No Hit
CGTGGGGGGCCGGGCCA468430.12197588063211123No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCG462310.12038227563356606No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCG446990.11639305527773071No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGT438110.1140807656719985No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCG425960.11091699104253379No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT424950.1106539941391791No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTAT421040.10963585761233079No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG414130.107836542164627No Hit
TCTACAGTCCGACGATC411130.10705536324377152Illumina DpnII expression Sequencing Primer (100% over 17bp)
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC410690.10694079033537937No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGG408330.10632626291763973No Hit
CCGTGGCGCAATGAAGGTGAAGGCCGG387520.10090748513663886No Hit
TTCTACAGTCCGACGATC384770.100191404459188Illumina DpnII expression Sequencing Primer (100% over 18bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)