FastQCFastQC Report
Tue 5 Sept 2023
SRR12764764_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764764_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25175115
Total Bases772.7 Mbp
Sequences flagged as poor quality0
Sequence length15-44
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG7948003.1570858762710716No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA5498302.1840218008934613No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG4366441.7344270324087894No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC4350891.728250297962889No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA4202551.6693270318725455No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG2887871.1471129327512506No Hit
GCCGGGCGCGGTGGCGCGT2818961.1197406645411552No Hit
ATCGCATGATGAGTAATAGAAAGGAGCTT2683561.0659573948321586No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT2532401.0059139749709187No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG2382470.9463591328182611No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT2212080.8786772175618662No Hit
CGACTGCATAATTTGTGGTAGTGGGGG2063180.8195315095879403No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1771780.7037822865953144No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1677730.6664239666829724No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC1650330.6555402030934119No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG1522940.6049386467549404No Hit
ACAAGCGGTGGAGCATGTGGTTTAATTTG1397660.5551752196563948No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA1315140.5223968192399518No Hit
AGCTTCACTTAAAAATGGGGGTGCGGAACA1193140.47393626603095956No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1118690.44436341204399665No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA1114430.44267126485817443No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG1090440.43314201345257014No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1058310.42037941038203797No Hit
GACTGCATAATTTGTGGTAGTGGGGGA1030190.40920965008501453No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTC994880.3951838948898545No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTC956780.3800499024532758No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCT918170.36471332901557746No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC907950.36065376464020127No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC881410.35011160822899917No Hit
AGCGGGAAGCTTCCTCAAAGATGAGTATTCCT850850.33797263686779583No Hit
TATCAACCTGTACCGAGAACGAACACACGTTCCCAA829610.32953573399764013No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTG759890.30184171949164884No Hit
AGTTCAACCTTGAGCTTAC746390.2964792812267193No Hit
GTTGGTCCGAGTGTTGTGGGTTATTGTTA738680.2934167331509707No Hit
TAGTTCAACCTTGAGCTTAC733900.2915180327875364No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC728060.28919828171589285No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGG701450.278628320069243No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT692950.27525197005058366No Hit
CCCCACAACCGCGTTTGACTAGCTTGACATTTT614190.24396710799533586No Hit
GACTGCATAATTTGTGGTAGTGGGGG605420.24048350921137798No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT604240.24001479238525822No Hit
TATCAACCTGTACCGAGAACGAACACACGTTCCCA604140.2399750706203328No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG602060.2391488579098844No Hit
CAAGCGGTGGAGCATGTGGTTTAATTTG592460.2353355684770457No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT569150.2260764250729341No Hit
TTAGTTCAACCTTGAGCTTAC523440.20791960632553216No Hit
GGAGTGGACGTACCGCTGGTGTTCCAGTT522710.20762963744157675No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG502460.19958598004418251No Hit
TCGACTGCATAATTTGTGGTAGTGGGG493080.1958600784941797No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTA492040.19544697213895548No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG482980.1918481802367139No Hit
GCTGGTCCGATGGTAGTGGGTTATCAGAA454360.18047981111506342No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCG452430.17971318105200312No Hit
TCGACTGCAAAATTTGTGGTAGTGGGGGA451400.17930404687327148No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG450790.17906174410722653No Hit
CCCCACAACCGCGTTTGACTAGCTTGACATTT445570.17698826797812045No Hit
GTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG438410.17414418960946157No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG430650.17106178065125027No Hit
CGACTGCATAATTTGTGGTAGTGGGG430650.17106178065125027No Hit
CAGCTTCACTTAAAAATGGGGGTGCGGAACA418830.16636666803706757No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCC413130.16410252743631956No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCT402640.15993571429564474No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG401660.15954644099937576No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT391910.15567356891914894No Hit
GGCGCCGGCGCGCCCCGCTCGCTCCGCCGTCG385220.15301618284563945No Hit
CGACTGCAAAATTTGTGGTAGTGGGGGA379240.1506408213031003No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCC377420.14991788518145796No Hit
AAGCGGGAAGCTTCCTCAAAGATGAGTATTCCT365670.14525057780272305No Hit
CCCCCCGCGGGGGCGCGCCGGC364650.14484541580048393No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCG362730.14408275791391617No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT354750.140912961072869No Hit
AGACCGGGGTTCGATTCCCCGACGGGGAGC350290.13914137035719598No Hit
GTTGGTCCGAGTGTTGTGGGTTATTGTT348350.13837076811764315No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTC347620.1380807992336877No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATT342410.1360112952810742No Hit
AGCGGGAAGCTTCCTCAAAGATGAGTATTCCTT340490.13524863739450643No Hit
AGACCGGGGTTCGATTCCCCGACGGGGA337220.13394973568144575No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC333210.13235689290793706No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC331820.13180476037547395No Hit
TGGAAACAATAGCTAATACCGGATATAGTTATTT330310.13120496172510035No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC329140.13074021707547315No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG326770.12979881124674109No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC322940.12827746765009812No Hit
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGG318600.1265535430523356No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT315920.1254889997523348No Hit
CTTTAAAATCTGTGTGGCTGTCACTCGGCT300290.11928048789449422No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT287460.11418418545456495No Hit
TTGGAAACAATAGCTAATACCGGATATAGTTATTT280020.11122888614411493No Hit
ATCTTGAGTTGTGATGCATAGTGA274780.10914746566202378No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGG274460.10902035601426249No Hit
ATCTTGAGTTGTGATGCATAGTGAAG273490.10863505489448608No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCG266720.10594589140903628No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC262030.10408294063403484No Hit
TAAGCGGGAAGCTTCCTCAAAGATGAGTATTCCT257730.10237490474224249No Hit
TTATCAACCTGTACCGAGAACGAACACACGTTCCCAA251950.10007898672955416No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)