FastQCFastQC Report
Tue 5 Sept 2023
SRR12764777_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764777_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9112514
Total Bases270.9 Mbp
Sequences flagged as poor quality0
Sequence length15-50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT2888963.1703216038954785No Hit
CAGAGTTCTACAGTCCGACGATC1686371.8506089537969432Illumina DpnII expression Sequencing Primer (100% over 23bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCT1611311.7682387099761931No Hit
GTTCTACAGTCCGACGATC1238331.3589334403217377Illumina DpnII expression Sequencing Primer (100% over 19bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCC1206521.3240254006742815No Hit
AGAGTTCTACAGTCCGACGATC1185111.300530237868496Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTACAAATTCTAGAATTAAAGCATCTATGC1094751.2013698963864416No Hit
CCCCGCGCAGGTTCGTATCCTGC1048741.150878890282089No Hit
AGTTCTACAGTCCGACGATC934301.025293349343551No Hit
TCTACAGTCCGACGATC872560.9575403670161714Illumina DpnII expression Sequencing Primer (100% over 17bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC804020.8823251190615454No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGC755810.8294198505483777No Hit
TTCTACAGTCCGACGATC683080.7496065300969633Illumina DpnII expression Sequencing Primer (100% over 18bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGA682830.7493321820959618No Hit
GTCAGGATGGCCGAGCGGTCTAAGGCTGCGTT613200.6729207768569684No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG604920.6638343710637921No Hit
ATCCATTGGGGTTTCCCCGCGCAGGTTCGTATCCTGC597630.6558343833545824No Hit
CTACAGTCCGACGATC551650.60537629901035Illumina DpnII expression Sequencing Primer (100% over 16bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGC549170.6026547668404131No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGA535750.5879277661466419No Hit
TTGCTGGCATACTAATTGTTA527210.5785560384324238No Hit
TGCTGGCATACTAATTGTTA503870.5529429090589052No Hit
GCATTGGTGGTTCAGTGGTAGAATTCT495800.5440869555865703No Hit
ATGATGAACCGACGA493570.5416397714176352No Hit
GAGTTCTACAGTCCGACGATC493190.5412227624561126Illumina DpnII expression Sequencing Primer (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCG487710.5352090542741553No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCG468590.5142269191575453No Hit
GGGGATGTAGCTCAGTGGTAGAGCATGCTT439550.4823586553611879No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG428100.46979351691531007No Hit
GGTTCCATAGTGTAGTGGTTATCACGTCTGCTTT411610.451697522769238No Hit
AAGTACAAATTCTAGAATTAAAGCATCTATGC397830.43657546095402433No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC391980.43015571773058453No Hit
CTTTGCTGGCATACTAATTGTTA385700.42326409594542186No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGG368860.4047840145979474No Hit
GGTAGCGTGGCCGAGCGGTCTAAGGCTCTGGATT366760.4024794913895331No Hit
GGTAGCGTGGCCGAGCGGTCTAAGGCCTGGATT357870.3927236764739127No Hit
TTTGCTGGCATACTAATTGTTA353700.38814755181720434No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT351030.38521751516650615No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCCTT339060.3720817328785448No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGC323420.3549185219358785No Hit
TACAGTCCGACGATC316020.3467978211062282Illumina DpnII expression Sequencing Primer (100% over 15bp)
GGTAGCGTGGCCGAGCGGTCTAAGGCTGGATT302240.33167575929101456No Hit
CCCCGCGCAGGTTCGAATCCTGC300520.32978824504412285No Hit
CCCCGCGCAGGTTCGGATCCTGC290450.3187375075637744No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGA286110.3139748262663849No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC273780.30044398285698104No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG273080.29967580845417635No Hit
TTCTTTGCTGGCATACTAATTGTTA259010.28423550295780065No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAC257840.2829515543131127No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTT255620.28051534406421763No Hit
GTCAGGATGGCCGAGCGGTCTAAGGCTCTGCGTT252420.2770036896513959No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT244030.26779657073777885No Hit
TTTCTTTGCTGGCATACTAATTGTTA242950.26661138737345147No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGG238200.26139877535441924No Hit
ATTGGGGTTTCCCCGCGCAGGTTCGTATCCTGC236790.25985145262876963No Hit
TGGCATACTAATTGTTA236280.25929178270672615No Hit
GGCTCGTTGGTCTAGGGGTATGATTCTCGCTT236190.2591930174263655No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGG220470.2419420151233787No Hit
TTTTCTTTGCTGGCATACTAATTGTTA219240.24059222295845034No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAC218440.23971430935524488No Hit
GACCCAGTGGCCTAATGGATAAGGCATCAGCCT214990.23592830694142142No Hit
ATCCATTGGGGTTTCCCCGCGCAGGTTCGGATCCTGC209980.2304303730013474No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT207090.22725891010976773No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCG191930.21062244732902466No Hit
TCCTCGTTAGTATAGTGGTGAGTATCCCCGCCTGT185760.20385153866430272No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGACT183440.20130558921500696No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCC181560.1992424922474742No Hit
GGTTCCATAGTGTAGTGGTTATCACGTCTGCTT181270.19892424856631222No Hit
TATGATGAACCGACGA178370.19574181175469252No Hit
GTAGTCGTGGCCGAGTGGTTAAGGCAATGGA178320.19568694215449217No Hit
CTGGCATACTAATTGTTA172240.18901479877013083No Hit
ATCCATTGGGGTTTCCCCGCGCAGGTTCGAATCCTGC170830.18746747604448125No Hit
GGTAGCGTGGCCGAGCGGTCTAAGGCGCTGGATT170290.18687488436231758No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG166610.18283648178757256No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTC164230.18022468881803638No Hit
CAAATTCTAGAATTAAAGCATCTATGC163490.17941261873507136No Hit
GTCAGGATGGCCGAGCGGTCTAAGGCCTGCGTT163340.17924800993447035No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC162970.17884197489298784No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGT161610.17734952176753857No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGG160460.17608752096293076No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC154600.16965680381945092No Hit
GCATGGGTGGTTCAGTGGTAGAATTCT151160.16588177532566753No Hit
GGGGATGTAGCTCAGTGGTAGAGCATGCT149000.16351140859701285No Hit
GGTTCCATAGTGTAGCGGTTATCACGTCTGCTTT148120.1625457036334869No Hit
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCAC145000.15912184058098566No Hit
TCCTTGGTATAGTGGTGAGTATCCCCGCCTGT144490.15856217065894218No Hit
GTCAGGATGGCCGAGCGGTCTAAGGCGCTGCGTT144080.1581122399372994No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTT143970.15799152681685866No Hit
GACGAGGTGGCCGAGTGGTTAAGGCAATGGA142560.1564442040912091No Hit
ATGGAACCAATTTATGATGAACCGACGA141410.15518220328660126No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGC139220.15277891479782638No Hit
GCCTGGGTAGCTCAGTCGGTAGAGCATCAGA137930.15136327911265762No Hit
TCTTTGCTGGCATACTAATTGTTA136320.14959647798620668No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCG135930.149168495104644No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTT135760.14898193846396285No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA135730.14894901670384264No Hit
GACCCAGTTGCCTAATGGATAAGGCATCAGCCT133700.14672131093570884No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTG129860.14250732564032276No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGT129610.14223297763932105No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGG121320.13313559792610472No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGT119090.13068841375716952No Hit
TCCTCGTATAGTGGTGAGTATCCCCGCCTGT115010.1262110543808218No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTT115010.1262110543808218No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTT114230.12535508861769648No Hit
ATTTTCTTTGCTGGCATACTAATTGTTA113880.12497100141629412No Hit
TTATGATGAACCGACGA110410.12116305116239054No Hit
GCGTTGGTGGTATAGTGGTTAGCATAGCTGCCTT108520.11908898027481768No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGC106390.11675153530628321No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGG103520.11360202025478369No Hit
AAATCCATTGGGGTTTCCCCGCGCAGGTTCGTATCCTGC100830.11065003576400541No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGG99310.10898199991791507No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCT98880.10851012135619216No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGT98420.10800532103434904No Hit
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGG97690.10720422487142406No Hit
TGGAACCAATTTATGATGAACCGACGA97330.10680916374998162No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTC96410.10579956310629536No Hit
AGCCTCTTCTCGTTCCTCATCA94140.10330848325719993No Hit
GTACAAATTCTAGAATTAAAGCATCTATGC93580.10269394373495612No Hit
AAATTCTAGAATTAAAGCATCTATGC92020.10098201220870551No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAG91610.10053208148706275No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGG91300.10019188996582062No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)