FastQCFastQC Report
Tue 5 Sept 2023
SRR12764778_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764778_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7082983
Total Bases212.5 Mbp
Sequences flagged as poor quality0
Sequence length15-50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAGTTCTACAGTCCGACGATC1415121.9979152851277493Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGAGTTCTACAGTCCGACGATC1097321.5492342703632072Illumina DpnII expression Sequencing Primer (100% over 22bp)
GTTCTACAGTCCGACGATC1080631.525670751998134Illumina DpnII expression Sequencing Primer (100% over 19bp)
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT924261.3049021859857635No Hit
AGTTCTACAGTCCGACGATC767981.0842606850814127No Hit
TCTACAGTCCGACGATC751901.0615583857818098Illumina DpnII expression Sequencing Primer (100% over 17bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG707240.9985058555131363No Hit
TTCTACAGTCCGACGATC569170.8035738614648659Illumina DpnII expression Sequencing Primer (100% over 18bp)
CTACAGTCCGACGATC499300.7049289826052103Illumina DpnII expression Sequencing Primer (100% over 16bp)
GAGTTCTACAGTCCGACGATC414580.5853183609222273Illumina DpnII expression Sequencing Primer (100% over 21bp)
CTCGAAGGCATTCAGTACGGTCGTAGTGGTGA308020.4348732730263506No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGG300430.42415744891664997No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG297470.41997841869732006No Hit
AAACGCTTCGGGCCCCGCGG295700.4174794715729234No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCT287040.40525298451231634No Hit
TTTACTATTAGTGTTACCACAGAAATTCTA286580.40460354062688No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGG284950.4023022503371814No Hit
TACAGTCCGACGATC284570.4017657532144296Illumina DpnII expression Sequencing Primer (100% over 15bp)
TTACTATTAGTGTTACCACAGAAATTCTA277970.3924476452929507No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG275640.38915807082976195No Hit
TTTTACTATTAGTGTTACCACAGAAATTCTA261360.3689970736905623No Hit
TTGGCAGAGACATTGCT242470.34232751935166295No Hit
TGGCAGAGACATTGCT242040.3417204305022333No Hit
AGCTAACTGGTTATATATT229690.3242842740127994No Hit
TTTGGCAGAGACATTGCT218820.3089376326330305No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCG215220.3038550283122238No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGG207770.2933368610372212No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGG202470.285854138009367No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGG196720.2777360894414119No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCC177250.2502476710730493No Hit
AGCAGTTGACCCGGGTTCGTTTCCCGGCCAACG172530.24358381207465837No Hit
CGCCCGGGGCACCCCCCTCGCGGC170070.2401106991221072No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG170000.24001187070475816No Hit
GGGGATGTAGCTCAGTGGTAGAGCATGCTT168850.23838826099116714No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGC167720.23679288796824727No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGA155950.2201755955082767No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT154980.21880611601072597No Hit
TCGAAGGCATTCAGTACGGTCGTAGTGGTGA154820.21858022248535683No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGC154150.21763429334787335No Hit
TACTATTAGTGTTACCACAGAAATTCTA148180.20920564118253565No Hit
AATTTGGCAGAGACATTGCT144900.2045748239124674No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGA140230.1979815566407543No Hit
AGGGGGTCCCCCCCGCGGGGGCGCGCCGGC128170.18095483216605207No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC127850.1805030451153137No Hit
CGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATG121930.1721449846766539No Hit
TAACCCAGAGGTCGATGGATCGTAACCATCCTCTG119820.16916601381084778No Hit
GGTTCCATAGTGTAGTGGTTATCACGTCTGCTTT119710.16901071201215645No Hit
CGAAGGCATTCAGTACGGTCGTAGTGGTGA114580.161768000855007No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGG113090.15966436740000647No Hit
TAACCGAAAGGTTGGTGGTTCGTGCCCACCCAGGGACGC112320.15857725480916726No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG112040.15818194113977121No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG110690.1562759645194687No Hit
CAATTTGGCAGAGACATTGCT108700.15346641379768947No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGG105930.14955563213973547No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGG104810.1479743774621512No Hit
GGTAGCGTGGCCGAGCGGTCTAAGGCTCTGGATT104800.1479602591168156No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCAC101460.1432447317747339No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT100960.1425388145079552No Hit
AAAGTAAACGCTTCGGGCCCCGCGG100130.14136699184510254No Hit
GGTAGCGTGGCCGAGCGGTCTAAGGCCTGGATT99600.14061871954231714No Hit
GTCAGGATGGCCGAGCGGTCTAAGGCTGCGTT97140.1371456065897659No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC94860.13392662385325504No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG94780.13381367709057046No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT94180.13296657637043602No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGG93740.13234536917567075No Hit
AGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCG92920.13118766485815367No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG91960.1298323037059386No Hit
ACCCCCTCCCCGCACCCCCGGCGACGGGGGC91080.12858988931640808No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATG89590.12648625586140755No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGG89520.1263874274440585No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAG88770.12532855154389047No Hit
ATTTGGCAGAGACATTGCT88090.12436850406107144No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC84710.11959650333764743No Hit
GGTAGCGTGGCCGAGCGGTCTAAGGCTGGATT84350.11908824290556676No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGT83030.117224621321271No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGACT82540.11653282239982787No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGG81770.11544570980898868No Hit
ACAGAGTTCTACAGTCCGACGATC79690.11250909397918928Illumina DpnII expression Sequencing Primer (95% over 24bp)
TAACCGAAAGGTTGGTGGTTCGTGCCCACCCAGGGACG78550.11089960261093383No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGC78170.11036310548818204No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG76980.10868302239324872No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCA76470.10796298678113445No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG76070.1073982529677115No Hit
ATTAGTGTTACCACAGAAATTCTA74740.10552051303808015No Hit
GCATTGGTGGTTCAGTGGTAGAATTCT74730.10550639469274457No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA74560.10526638282203982No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTT74510.10519579109536195No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCG72660.10258389720828075No Hit
ACTATTAGTGTTACCACAGAAATTCTA72320.10210387346687123No Hit
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG71390.10079086735066285No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)