FastQCFastQC Report
Tue 5 Sept 2023
SRR12764779_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764779_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18740076
Total Bases628.6 Mbp
Sequences flagged as poor quality0
Sequence length15-50
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT3841012.0496234913881883No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2670721.425138297197941No Hit
AGGAGCTTGCTCCGTCCACTCCAC2141771.1428822380442853No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCT1896101.0117888529374162No Hit
CTGAAGAACATGTCCAAATTCACA1488250.7941536629840775No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGA1317690.7031401580228384No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCC1282340.6842768407129193No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGG1224740.6535405726209434No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGA1180450.6299067303675823No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC1023440.5461237190286742No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1009380.5386210813659453No Hit
GGTTCCATAGTGTAGTGGTTATCACGTCTGCTTT994470.5306648703025538No Hit
TAATCTCAGGGTCGTGGGTTCGTGCCCCACGTTGGGCGCCA934120.49846115885549236No Hit
TGAAGAACATGTCCAAATTCACA898060.4792189743520784No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGC841470.4490216581832432No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT794940.4241925166151941No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCG779170.4157773959934848No Hit
TAATCTCAGGGTCGTGGGTTCGGGCCCCACGTTGGGCGCCA773300.41264507145008383No Hit
TAACCGAAAGGTTGGTGGTTCGTGCCCACCCAGGGACGC749400.3998916546549758No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG686500.3663272230059259No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGC640300.34167417464048705No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG602660.3215888772276057No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC559950.2987981478837119No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG555920.29664767634880457No Hit
CAGAGTTCTACAGTCCGACGATC553770.29550040245301035Illumina DpnII expression Sequencing Primer (100% over 23bp)
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGG522340.27872885894379507No Hit
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCAC502780.268291334570895No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG501980.26786444195850645No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCCTT497520.2654845156444403No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGG492300.26269904134860494No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC482610.2575283045810487No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG451870.24112495595001857No Hit
TCCTCGTTAGTATAGTGGTGAGTATCCCCGCCTGT448610.23938536855453524No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCAC434520.23186672241884188No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGG430790.22987633561358023No Hit
GGTTCCATAGTGTAGCGGTTATCACGTCTGCTTT430530.22973759551455394No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCG429540.2292093159067231No Hit
TAATCCCAGGGTCGTGGGTTCGTGCCCCACGTTGGGCGCCA420670.22447614406686506No Hit
CGCAGAAGGTCCTGGGTTCGAGCCCCAGTGGAACCACCA418330.22322748317562852No Hit
GGCTCGTTGGTCTAGGGGTATGATTCTCGCTT415320.2216212997215166No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC410870.21924670956510525No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGA408380.21791800630904593No Hit
TAACCGAAAGGTTGGTGGTTCGGGCCCACCCAGGGACGC397150.21192550126264165No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGG395090.2108262527857411No Hit
AGAGTTCTACAGTCCGACGATC389620.2079073745485344Illumina DpnII expression Sequencing Primer (100% over 22bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGG371050.1979981297834651No Hit
GGGGATGTAGCTCAGTGGTAGAGCATGCTT363030.1937185313442699No Hit
CGCAGAAGGTCCTGGGTTCGAGCCCCAGTGGAACCAC362930.19366516976772133No Hit
GTTCTACAGTCCGACGATC353380.18856913920733298Illumina DpnII expression Sequencing Primer (100% over 19bp)
GGTAGCGTGGCCGAGCGGTCTAAGGCCTGGATT330730.17648274211908213No Hit
GCATTGGTGGTTCAGTGGTAGAATTCT321780.1717068810179852No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAC313260.16716047469604714No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGC308230.1644763873956541No Hit
TAATCTCAGGGTCGTGGGTTCGTGCCCCACGTTGGGCGC306030.1633024327115856No Hit
TAACCGAAAGGTTGGTGGTTCGTGCCCACCCAGGGACGCCA298050.1590441789030098No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAC295690.15778484569646356No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT288490.1539428121849666No Hit
CGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATG288010.15368667661753346No Hit
GGTAGCGTGGCCGAGCGGTCTAAGGCTGGATT277610.1481370726564823No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGACT274110.14626941747728237No Hit
CAGAGTTCTACAGTCCGA273860.14613601353591096Illumina DpnII expression Adapter 1 (100% over 18bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG273790.14609866043232697No Hit
TAACCGAAAGGTTGGTGGTTCGTGCCCACCCAGGGACG273050.14570378476586754No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGC272150.14522353057693044No Hit
GCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT264060.1409065790341512No Hit
TCCTTGGTATAGTGGTGAGTATCCCCGCCTGT258980.1381958109454839No Hit
AGTTCTACAGTCCGACGATC258860.13813177705362562No Hit
GGTAGCGTGGCCGAGCGGTCTAAGGCTCTGGATT253190.13510617566332175No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTT253030.13502079714084403No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGG247610.1321285996919116No Hit
TAGGAGCTTGCTCCGTCCACTCCAC247270.13194717033164646No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGG244660.1305544331837288No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC243470.12991943042280085No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTT242080.12917770450877575No Hit
TAATCTCAGGGTCGTGGGTTCGGGCCCCACGTTGGGCGC241270.12874547573873235No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGC240220.12818517918497235No Hit
AGCAGTTGACCCGGGTTCGTTTCCCGGCCAACG237810.12689916519015185No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGG235360.1255918065647119No Hit
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCACCA235100.12545306646568563No Hit
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG234330.12504218232626166No Hit
AGCAGTTGACCCGGGTTCGTTTCCCGGCCAACGCACCA232670.12415638015555538No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCG229000.12219801029622292No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA228940.12216599335029378No Hit
TCCTCGTATAGTGGTGAGTATCCCCGCCTGT227170.1212214934453841No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAG226650.12094401324733155No Hit
TCTACAGTCCGACGATC224160.11961530999127219Illumina DpnII expression Sequencing Primer (100% over 17bp)
GGTTCCATAGTGTAGTGGTTATCACGTCTGCTT221690.11829727905052254No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCACCA220110.11745416614105514No Hit
GACCCAGTGGCCTAATGGATAAGGCATCAGCCT219400.1170752989475603No Hit
ATCAGAAGATTCCAGGTTCGGCTCCTGGCTGGCTCGC218710.11670710406937518No Hit
TCCTCGTTAGTATAGTGGTGAGTATCCCCGCCTG218320.11649899392083575No Hit
CTAGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGA212810.11355877105300961No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT211240.11272099430119707No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTT209370.11172313281973884No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG208430.11122153400018228No Hit
CCGCCGCGGCCCGGGTTCGATTCCCGGTCAGG206620.11025568946465318No Hit
CTAGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGA206400.11013829399624632No Hit
TAATCTCAGGGTCGTGGGTTCGTGCCCCACGTTGGGCGCC200750.10712336492125218No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC200530.10700596945284534No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGC200250.10685655703850934No Hit
AGCAGTTGACCCGGGTTCGTTTCCCGGCCAACGCAC197770.10553318994010483No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT196760.10499423801696428No Hit
AGAGTTCTACAGTCCGA196250.10472209397656658Illumina DpnII expression Adapter 1 (100% over 17bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGG190410.10160577790613015No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT189320.10102413672175076No Hit
ATCAGAAGATTGAGGGTTCGTGTCCCTTCGTGGTCGC188120.10038379780316793No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)