FastQCFastQC Report
Tue 5 Sept 2023
SRR12764780_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764780_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19310811
Total Bases666.3 Mbp
Sequences flagged as poor quality0
Sequence length15-50
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG5928183.0698762470411003No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGG2350361.2171213316727092No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2064411.0690436564264443No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT1823410.9442430978170725No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT1694780.8776327415767261No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC913430.47301483091518015No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCT753980.390444502822797No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC670190.3470543003087752No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG669150.3465157418815813No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGA644890.3339528308779989No Hit
CAGAGTTCTACAGTCCGACGATC604470.31302155046724867Illumina DpnII expression Sequencing Primer (100% over 23bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGA578140.29938670105569365No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGG576720.29865136166471723No Hit
GCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT567710.2939855814445079No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG505660.26185332143740625No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGG486720.2520453439267776No Hit
GGTTCCATAGTGTAGTGGTTATCACGTCTGCTTT485570.25144982258901505No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGG476090.24654065538728542No Hit
CTGTCCCCGCCGGCGGGCCGCCTCGC467240.24195773030972131No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGC439800.22774807334606503No Hit
TAATCTCAGGGTCGTGGGTTCGTGCCCCACGTTGGGCGCCA412770.2137507326854372No Hit
CCCCCGGGGAGCCCGGCGGGCGCCGGCGCGCCCCGCTCGCTCCGCCGTCG398230.20622127159755227No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG388470.20116710789619346No Hit
ATGACTTCTTTAAGTTTAGAATAGACG386890.20034891336257188No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCG363670.18832456078618345No Hit
CGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCC353680.18315129281727213No Hit
TAATCTCAGGGTCGTGGGTTCGGGCCCCACGTTGGGCGCCA345750.17904478480991812No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT340880.17652288140565406No Hit
TAACCGAAAGGTTGGTGGTTCGTGCCCACCCAGGGACGC338610.17534737406937492No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG332800.17233869670207014No Hit
AGAGTTCTACAGTCCGACGATC329680.17072302142048826Illumina DpnII expression Sequencing Primer (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG329390.17057284647444376No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC328740.17023624745744753No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGG327360.16952162185213246No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC325770.16869824887209553No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTC324140.1678541621063973No Hit
AGGGGGTCCCCCCCGCGGGGGCGCGCCGGC312710.16193519785367896No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCT307130.1590456247539267No Hit
GTTCTACAGTCCGACGATC306230.15857956457654732Illumina DpnII expression Sequencing Primer (100% over 19bp)
GCATTGGTGGTTCAGTGGTAGAATTCT304890.15788565275689354No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCG296100.15333379835782143No Hit
CGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCC294650.15258292362759907No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGG289380.14985388236672192No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC268910.13925360255454833No Hit
CGCCCGGGGCACCCCCCTCGCGG268380.138979144894536No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC266000.13774667464768828No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG256080.13260965580368428No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC251910.13045024364849306No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCG246040.12741049560269635No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGG244100.12640587699812295No Hit
TGACTTCTTTAAGTTTAGAATAGACG244080.1263955201052923No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCG239330.12393575805801217No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG233110.120714764387679No Hit
AGGGGGTCCCCCCCGCGGGGGCGCGCCGGCG231330.11979300092575086No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGG231020.11963246908687573No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC230820.1195289001585692No Hit
AGTTCTACAGTCCGACGATC230450.11933729764120211No Hit
GGTTCCATAGTGTAGCGGTTATCACGTCTGCTTT228040.11808929205510842No Hit
GGCTCGTTGGTCTAGGGGTATGATTCTCGCTT224430.11621987289917549No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC222360.11514793449120288No Hit
CTCCTCGCCGCGCGGCGGCGGTGGCGGCGGGC219840.11384296599454057No Hit
ACCCCCTCCCCGCACCCCCGGCGACGGGGGC219310.11356850833452825No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG219020.1134183333884838No Hit
CCCCGGGGAGCCCGGCGGGCGCCGGCGCGCCCCGCTCGCTCCGCCGTCG213030.11031644398570314No Hit
TAATCCCAGGGTCGTGGGTTCGTGCCCCACGTTGGGCGCCA211960.1097623502192632No Hit
CGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATG210970.10924968402414585No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCC210410.10895969102488756No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGA207790.10760293806407199No Hit
TCTACAGTCCGACGATC204300.105795660265123Illumina DpnII expression Sequencing Primer (100% over 17bp)
CTAGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGA203910.10559370085492525No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGC202620.10492568126734812No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGG200730.1039469548948514No Hit
GCTCGCCGGCCGAGGTGGGATCCCGAGGCCTC200600.10387963509145215No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGG197570.10231056582760817No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)