FastQCFastQC Report
Tue 5 Sept 2023
SRR12764781_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764781_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29678661
Total Bases997.6 Mbp
Sequences flagged as poor quality0
Sequence length15-50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG6637122.236327306006157No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG3531671.1899694531367166No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT2535300.8542501294111617No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT1933350.6514276368465545No Hit
ATTAATTGCCAGGAACCTAAATTGG1763730.5942754627643073No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGG1490790.502310397359234No Hit
GCCGGGCGCGGTGGCGCGTGCCT1483810.49995853923463734No Hit
AATTAATTGCCAGGAACCTAAATTGG1375330.4634070250002182No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC1295670.43656619144644027No Hit
TTAATTGCCAGGAACCTAAATTGG1273180.42898835631432297No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGG1226550.4132767310492882No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC1159930.3908296267139545No Hit
AGTACAAATTCTAGAATTAAAGCATCTATG1073190.3616032407931072No Hit
CCCCGCGCAGGTTCGTATCCTG1021110.34405527931330865No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG995750.3355104194222239No Hit
AATTGCCAGGAACCTAAATTGG894080.3012534831001978No Hit
CTGTCCCCGCCGGCGGGCCGCCTCGC860220.2898446125989309No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGG794580.26772771183982996No Hit
AGGGGGTCCCCCCCGCGGGGGCGCGCCGGC792080.2668853557780117No Hit
CAGAGTTCTACAGTCCGACGATC788010.26551400010937154Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG781670.2633777851366003No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT694110.2338751064274766No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGC624070.21027565899957548No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG602430.2029842249284764No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG591500.19930144422620685No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC590180.1988566802255668No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCT587000.1977852033149339No Hit
CCTCCGAACAATCCATGCT578080.19477967688636627No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCG559770.18861026108960913No Hit
GTTCTACAGTCCGACGATC559450.18850243951369638Illumina DpnII expression Sequencing Primer (100% over 19bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGG544480.1834584114155285No Hit
CCCCCGGGGAGCCCGGCGGGCGCCGGCGCGCCCCGCTCGCTCCGCCGTCG544100.1833303732941321No Hit
AGAGTTCTACAGTCCGACGATC538970.18160185865528097Illumina DpnII expression Sequencing Primer (100% over 22bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGG517860.17448900406928736No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC499820.1684105627272066No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGG495850.16707290130103916No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC493300.1662136981179845No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGG488830.1647075654794534No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGA485110.16345413965946778No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGG469490.15819109898522712No Hit
ATCCATTGGGGTTTCCCCGCGCAGGTTCGTATCCTG431980.14555238863370554No Hit
TAATTGCCAGGAACCTAAATTGG424660.14308597008470159No Hit
TCTACAGTCCGACGATC421950.14217285611369057Illumina DpnII expression Sequencing Primer (100% over 17bp)
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG419720.14142147450654866No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGG418100.14087562777849041No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTG411490.13864843835104285No Hit
AGTTCTACAGTCCGACGATC404260.13621234462026435No Hit
CCCCGGGGAGCCCGGCGGGCGCCGGCGCGCCCCGCTCGCTCCGCCGTCG404240.13620560577176982No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC397110.13380320628346407No Hit
AAGTACAAATTCTAGAATTAAAGCATCTATG388450.13088528488532553No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC384030.12939599936803078No Hit
GCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT379150.1277517203353615No Hit
AGACCGGGGTTCGATTCCCCGACGGGGAGC376570.126882408879565No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT374270.1261074413026922No Hit
GGTTCCATAGTGTAGTGGTTATCACGTCTGCTTT361950.12195631063005166No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCG358860.12091515853764427No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGC358730.12087135602242972No Hit
GCATTGGTGGTTCAGTGGTAGAATTCT352590.118802529534604No Hit
CAATTAATTGCCAGGAACCTAAATTGG347730.11716498935042925No Hit
TGGGAAACTCGACTGCATAATTTGTGGTAGTGGGGGA343450.11572287577259635No Hit
TAACCGAAAGGTTGGTGGTTCGTGCCCACCCAGGGACGC339450.11437510607368707No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG339280.11431782586148342No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT335260.11296331731407963No Hit
CGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATG332810.11213780837349772No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTC331250.1116121781909231No Hit
TAATCTCAGGGTCGTGGGTTCGTGCCCCACGTTGGGCGCCA329460.1110090512506612No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCG329350.1109719875839412No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG325420.10964780385476286No Hit
AAACCTCCGAACAATCCATGCT317030.1068208569113007No Hit
TTCTACAGTCCGACGATC312470.10528439945454411Illumina DpnII expression Sequencing Primer (100% over 18bp)
AGGGGGTCCCCCCCGCGGGGGCGCGCCGGCG309280.10420955311966398No Hit
CTACAGTCCGACGATC307200.10350871287623116Illumina DpnII expression Sequencing Primer (100% over 16bp)
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTC305420.10290895536021656No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCG304860.10272026760236926No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)