FastQCFastQC Report
Tue 5 Sept 2023
SRR12764782_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764782_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14199519
Total Bases445.4 Mbp
Sequences flagged as poor quality0
Sequence length15-50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAGTTCTACAGTCCGACGATC4193562.953311305826627Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGAGTTCTACAGTCCGACGATC3003212.1150082618995754Illumina DpnII expression Sequencing Primer (100% over 22bp)
GTTCTACAGTCCGACGATC2914332.052414592353445Illumina DpnII expression Sequencing Primer (100% over 19bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2519561.7743981327818217No Hit
AGTTCTACAGTCCGACGATC2188831.5414817924466315No Hit
TCTACAGTCCGACGATC1992481.4032024605903903Illumina DpnII expression Sequencing Primer (100% over 17bp)
TTCTACAGTCCGACGATC1635041.1514756239278245Illumina DpnII expression Sequencing Primer (100% over 18bp)
CTACAGTCCGACGATC1346130.948010985442535Illumina DpnII expression Sequencing Primer (100% over 16bp)
GAGTTCTACAGTCCGACGATC1179710.8308098323612229Illumina DpnII expression Sequencing Primer (100% over 21bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGG1071180.7543776658913587No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1022040.7197708598439144No Hit
TACAGTCCGACGATC774520.5454550960493803Illumina DpnII expression Sequencing Primer (100% over 15bp)
ATCCAGCGATCCGAGTTC663980.4676073886728135No Hit
TAAACCAGGGGTCGCGAGTTC571180.40225306223400953No Hit
AGACCGGGGTTCGATTCCCCGACGGGGAGC410660.28920697947585405No Hit
AGACCGGGGTTCGATTCCCCGACGGGGAG320260.22554285113460534No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGG314710.22163426803400876No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG274600.1933868323286162No Hit
AGGGGGTCCCCCCCGCGGGGGCGCGCCGGC245980.17323122001526953No Hit
ACCCCCTCCCCGCACCCCCGGCGACGGGGGC220910.15557569238789004No Hit
CCCCGGGGAGCCCGGCGGGCGCCGGCGCGCCCCGCTCGCTCCGCCGTCG211870.14920927955376515No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG210020.14790641852023298No Hit
CAGAGTTCTACAGTCCGA208990.14718104183669883Illumina DpnII expression Adapter 1 (100% over 18bp)
ACAGAGTTCTACAGTCCGACGATC192130.13530740020137302Illumina DpnII expression Sequencing Primer (95% over 24bp)
GGCGCCGGCGCGCCCCGCTCGCTCCGCCGTCG190940.13446934364466853No Hit
CCCCCGGGGAGCCCGGCGGGCGCCGGCGCGCCCCGCTCGCTCCGCCGT189790.13365945705625662No Hit
AATCCAGCGATCCGAGTTC184460.12990580878126928No Hit
CAGAGTTCTACAGTC163220.11494755561790508Illumina DpnII expression Adapter 1 (100% over 15bp)
TGGGAAACTCGACTGCATAATTTGTGGTAGTGGGGGACTG161610.11381371439412842No Hit
CAGAGTTCTACAGTCCGACGAT159550.11236296102706013Illumina DpnII expression Sequencing Primer (100% over 22bp)
CCCCCGGGGAGCCCGGCGGGCGCCGGCGCGCCCCGCTCGCTCCGCCGTCG149300.10514440665208447No Hit
CCCCCGGGGAGCCCGGCGGGCGCCGGCGCGCCCCGCTCGCTCCGCCGTC147490.1038697155868449No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT147070.1035739309197727No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT143090.10077101907466021No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)