FastQCFastQC Report
Tue 5 Sept 2023
SRR12764783_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764783_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8369058
Total Bases253.4 Mbp
Sequences flagged as poor quality0
Sequence length15-44
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC7335598.765132228740677No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG6943138.296190562904451No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG3114433.7213626671006463No Hit
CAGAGTTCTACAGTCCGACGATC1660271.9838194453903895Illumina DpnII expression Sequencing Primer (100% over 23bp)
CGACTGCATAATTTGTGGTAGTGGGGG1298491.5515366245520106No Hit
AGAGTTCTACAGTCCGACGATC1268461.515654449999032Illumina DpnII expression Sequencing Primer (100% over 22bp)
GTTCTACAGTCCGACGATC1211761.447904889654248Illumina DpnII expression Sequencing Primer (100% over 19bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATT1111601.3282259484878705No Hit
CGACTGCATAATTTGTGGTAGTGGGG1046381.2502960309272562No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCG953531.1393516450716437No Hit
AGTTCTACAGTCCGACGATC900051.0754495906229828No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG801230.9573717854506445No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA779190.9310366829815255No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC770760.9209638647503697No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT734430.8775539612701931No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG680720.8133770849718093No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG636170.7601452875580501No Hit
TCTACAGTCCGACGATC624740.7464878365044191Illumina DpnII expression Sequencing Primer (100% over 17bp)
TTCTACAGTCCGACGATC587580.7020861846100241Illumina DpnII expression Sequencing Primer (100% over 18bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG513810.6139400635053551No Hit
GAGTTCTACAGTCCGACGATC506410.6050979692099159Illumina DpnII expression Sequencing Primer (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGG503440.6015491827156653No Hit
CCCCCGCGGGGGCGCGCCGG471270.5631099700826545No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTG447080.5342058807574281No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA418390.49992484219848876No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCT394280.4711163430818618No Hit
CCCCCGCGGGGGCGCGCCGGC374890.4479476662725961No Hit
CTACAGTCCGACGATC353010.42180374422067574Illumina DpnII expression Sequencing Primer (100% over 16bp)
AGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGTG336800.4024347782032338No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGG327850.39174062361618234No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG308650.36879897355234004No Hit
CCCCCCGCGGGGGCGCGCCGG308020.36804620065962024No Hit
AGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGT283380.3386044164110226No Hit
CCCCCCGCGGGGGCGCGCCGGC264420.31594953697297834No Hit
AAATTCTAGAATTAAAGCATCTATGC254070.30358255373543835No Hit
CCCCGGGGAGCCCGGCGGGCGCCGGCGCGCCCCGCT225490.26943295171332304No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG224070.2677362255106847No Hit
TTGGCAATTTTTGACAGTCTCCACGGAGACTG220090.2629806126328674No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG219730.2625504566941704No Hit
GCCGCTGGTGTAGTGGTATCATGCAAGATT212540.2539592866963044No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG209380.2501834734566304No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG208890.24959798342895936No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG207690.24816413029996923No Hit
GACTGCATAATTTGTGGTAGTGGGGG200630.2397282943910772No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGC191620.22896244714757621No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC186530.22288052012544302No Hit
GACTGCATAATTTGTGGTAGTGGGG181770.2171929027137821No Hit
CGTGATCGTATAGTGGTTAGTACTCTGC181120.2164162322689125No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTC181070.21635648838853788No Hit
TTGGCAATTTTTGACAGTCTCTACGGAGACTG178060.21275990678998757No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGG177730.21236559717951528No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC175390.20956958357798453No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGG166650.1991263532885063No Hit
TACAGTCCGACGATC166270.19867229979765944Illumina DpnII expression Sequencing Primer (100% over 15bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG166010.19836163161971154No Hit
TGGCAATTTTTGACAGTCTCTACGGAGACTG165660.19794342445708943No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATA162480.19414371366526556No Hit
CCGCTGGTGTAGTGGTATCATGCAAGATT155490.18579151918889797No Hit
CCCCGGGGAGCCCGGCGGGCGCCGGCGCGCCCCGCTCGCT155410.1856959289802986No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTG153750.18371243215186225No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC153680.18362879071933783No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC147790.1765909616112112No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGC146980.17562311074914286No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGC146100.17457161845455008No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGG144260.1723730436567652No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGT142980.1708436003191757No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCG134270.16043621635792224No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGG133420.15942057039155422No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCC132300.15808230747116342No Hit
AGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCG131590.15723394436984425No Hit
AAACCAATTTTTTGAGGCCTTGTTTCGGCAAGGCTA130150.15551332061505607No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT126980.15172555859930711No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGG123130.14712527981046375No Hit
CCCCGCGGGGGCGCGCCGG121710.1454285536078254No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATG119670.14299100328854217No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCG118480.1415690989356269No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG116320.13898816330344466No Hit
GAGCCCGGCGGGCGCCGGCGCGCCCCGCTCGCTCCGCCG115350.13782913202417763No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAG115080.13750651507015485No Hit
GACTGCATAATTTGTGGTAGTGGGGGA112820.1348060916772234No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGC111320.13301377526598573No Hit
CAAATTCTAGAATTAAAGCATCTATGC110780.13236854135794016No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATT108430.12956057898033446No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC106830.1276487748083476No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATAC105630.12621492167935747No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATA103290.12341890807782668No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGG102470.12243910843968342No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC101700.12151905268191475No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGG99440.11881862928898329No Hit
TGGCAATTTTTGACAGTCTCCACGGAGACTG97450.11644082285007464No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG97440.11642887407399972No Hit
TAAACCAATTTTTTGAGGCCTTGTTTCGGCAAGGCTA95820.11449317234986302No Hit
AGCCCGGCGGGCGCCGGCGCGCCCCGCTCGCTCCGCCG95310.11388378477004221No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGG94460.11286813880367419No Hit
CCCCGGGGAGCCCGGCGGGCGCCGGCGCGCCCCGC94370.11276059981899994No Hit
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGG94220.11258136817787617No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG93050.11118336137711077No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGG91950.10986899600886982No Hit
CGACTGCAAAATTTGTGGTAGTGGGGG91310.10910427434007507No Hit
AACCAATTTTTTGAGGCCTTGTTTCGGCAAGGCTA89590.1070490848551892No Hit
GTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTC88940.10627241441031954No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT88630.10590200235199708No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGT88550.10580641214339773No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT86950.10389460797141088No Hit
GTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGC85070.10164823806932631No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT84580.10106274804165534No Hit
CCCCGCGCAGGTTCGTATCCTGC84070.10045336046183453No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)