FastQCFastQC Report
Tue 5 Sept 2023
SRR12764784_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764784_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11915163
Total Bases367 Mbp
Sequences flagged as poor quality0
Sequence length15-44
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC6211735.213298382909239No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG5458444.581087140813768No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG4087673.430645472495844No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2098821.7614698179118489No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA2067181.7349154182783735No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA1558861.3082993493248896No Hit
CAGAGTTCTACAGTCCGACGATC1508791.2662772636849366Illumina DpnII expression Sequencing Primer (100% over 23bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG1314811.1034763015831173No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC1231001.0331373561570245No Hit
AGAGTTCTACAGTCCGACGATC1229681.0320295240610642Illumina DpnII expression Sequencing Primer (100% over 22bp)
CCCCCCGCGGGGGCGCGCCGGC1092680.9170499807682028No Hit
GTTCTACAGTCCGACGATC1070080.8980825524585774Illumina DpnII expression Sequencing Primer (100% over 19bp)
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG995050.8351123690041No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT955920.8022718614927886No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG899610.7550127513992045No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG889900.7468634713599805No Hit
CGACTGCATAATTTGTGGTAGTGGGGG789130.6622905620342752No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC783590.6576410242981988No Hit
AGTTCTACAGTCCGACGATC757430.6356858063964379No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT734920.6167939120933553No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTC685150.5750236064752114No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCT562570.4721462895639783No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG558660.46886475661306526No Hit
TCGACTGCATAATTTGTGGTAGTGGGG533200.447497025428859No Hit
CGACTGCATAATTTGTGGTAGTGGGG521720.43786224326096085No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC519480.4359822857647856No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG517310.4341610769403658No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT499960.41959979901240124No Hit
TCTACAGTCCGACGATC499170.4189367782883037Illumina DpnII expression Sequencing Primer (100% over 17bp)
TTCTACAGTCCGACGATC484500.40662473522183457Illumina DpnII expression Sequencing Primer (100% over 18bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATT482510.4049545944105003No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC464350.38971351042365093No Hit
GAGTTCTACAGTCCGACGATC453680.3807585343146376Illumina DpnII expression Sequencing Primer (100% over 21bp)
CGCCGGCCGAGGTGGGATCCCGAGGCCT441070.3701753807312581No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG439480.36884094661566946No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGG437790.3674225858261444No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA423800.3556812441424427No Hit
CCCCCCGCGGGGGCGCGCCGG418100.3508974237280682No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG374270.3141123625417462No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC371190.31152742098450525No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCG367150.30813678335747485No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC349290.2931474793924347No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT343390.2881958056301873No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGG340470.2857451467512446No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG336990.282824498498258No Hit
GACTGCATAATTTGTGGTAGTGGGGGA336530.28243843579815064No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG330320.2772265893467005No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG329700.27670624396829485No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTC328930.2760600085789846No Hit
CTACAGTCCGACGATC304310.2553972614558441Illumina DpnII expression Sequencing Primer (100% over 16bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGC304010.2551454814340349No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG303310.25455799471648016No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG299520.25137717377429075No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGG285000.23919102071872622No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG284630.23888049202516154No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG281720.23643822581361243No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGC279680.23472612166530998No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT273280.2293548145333807No Hit
CCCCCGCGGGGGCGCGCCGGC267620.22460456478858074No Hit
GACTGCATAATTTGTGGTAGTGGGGG266970.22405904140799415No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGG254380.213492673159402No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGG247040.2073324552924706No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGG246900.20721495794895967No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC239910.20134848344080564No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGG234050.1964303803481329No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG231090.19394615079961558No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC229080.19225922465349404No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGG222710.18691309552374566No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG218950.1837574525837372No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGG214410.17994718158702488No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG213130.17887292016063902No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC194390.16314506146495855No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC188000.1577821470004229No Hit
TCGACTGCAAAATTTGTGGTAGTGGGGGA182970.15356063530142225No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTC181180.15205834783796074No Hit
CTCGACTGCATAATTTGTGGTAGTGGGG180700.15165549980306606No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGC179450.15060641637886113No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG178780.15004410766348728No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG178710.1499853589917318No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC178680.14996018098955088No Hit
GTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTC177980.1493726942719961No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTC176560.1481809355020993No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGG174930.14681293071693605No Hit
GACTGCATAATTTGTGGTAGTGGGG174100.14611633932326398No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGG173860.14591491530581663No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG171360.14381674845740675No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC169770.14248231434181807No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCC165410.13882311135819123No Hit
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGG163390.13712779254467605No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCC163160.13693476119462233No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGG157040.13179844874971494No Hit
CGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT156860.13164738073662946No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGT156320.1311941766973729No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGT152860.12829031377917366No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG150370.1262005395981574No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC149010.12505913683262243No Hit
CGACTGCAAAATTTGTGGTAGTGGGGGA148160.12434576010416307No Hit
TACAGTCCGACGATC144520.12129082917287828Illumina DpnII expression Sequencing Primer (100% over 15bp)
CCCCCGGCCCGCCCCCGG143390.12034245775739702No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAG138040.11585238070179989No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTC137650.11552506667344795No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC137370.11529007198642603No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGG136550.11460187326014759No Hit
TCCTCGCCGCGCGGCGGCGGTGGCGGCGGG136460.11452633925360485No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC133320.11189104169200204No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTG130020.10912146145210098No Hit
ACGCGGCGCTCCCCCGGGGAGGGGGGAGGAC129990.10909628344992006No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCG129730.10887807409768545No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT122890.10313748960043602No Hit
TCCTCGCCGCGCGGCGGCGGTGGCGGCGGGC121310.10181144815224097No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCC120860.1014337781195272No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGC120260.10093021807590881No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCG119460.10025880468441765No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)