FastQCFastQC Report
Tue 5 Sept 2023
SRR12764785_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764785_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13866165
Total Bases419.4 Mbp
Sequences flagged as poor quality0
Sequence length15-44
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC3999422.884301463310151No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG2971242.1427986757693995No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT2126531.533610771255066No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2109771.5215237955123135No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG2086481.5047275147814843No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT1740331.2550910796171832No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA1681641.2127650291194427No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG1623241.170648120803409No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTT1592701.1486232855299212No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG1446971.043525733322804No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT1445131.0421987622388742No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1410871.0174911376000502No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA1302020.9389907014664833No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT1114080.803452144122041No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG1001180.7220309292439546No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT958150.6909985565583562No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT914720.6596777118979905No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCT892400.6435809757059721No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT867560.6256668660729192No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG858050.6188084448728254No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG836770.6034617358152019No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTC827080.5964735022264628No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT822650.5932786751059143No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG816360.5887424533026976No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC774900.5588423331180611No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG743060.5358799639265796No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTC741060.5344376040527428No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT732090.5279686200185848No Hit
CGACTGCATAATTTGTGGTAGTGGGGG730070.5265118365460096No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT728420.5253218896500943No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT695900.5018691181015082No Hit
CAGAGTTCTACAGTCCGACGATC683500.4929264868837202Illumina DpnII expression Sequencing Primer (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAA671520.4842867512394379No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAT670090.4832554639296446No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT666050.48034189698449425No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC638630.46056714311419206No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG623600.4497278086623086No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTT618360.4459488257928562No Hit
GTTCTACAGTCCGACGATC521750.37627563208717046Illumina DpnII expression Sequencing Primer (100% over 19bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC514720.37120573713063415No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGG496000.35770524871152193No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC486810.3510776050912419No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCC477870.34463025645519146No Hit
AGAGTTCTACAGTCCGACGATC474920.34250277564128223Illumina DpnII expression Sequencing Primer (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC474300.3420556440803928No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC465880.33598330901153994No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTG430090.3101722790692307No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC413700.2983521399031383No Hit
CGGTGGATCACTCGGCTCGTGC412840.2977319251573885No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG410220.29584243372266233No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCG379410.2736228798662067No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCG377870.2725122627633524No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCC373020.2690145400692982No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGG358530.2585646427833507No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG348190.25110764223561455No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTC334310.24109766471118727No Hit
AGTTCTACAGTCCGACGATC311210.22443840816837243No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG308200.2222676565582481No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG307000.22140224063394603No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGG303290.21872666306797878No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCC301300.21729151499351118No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG300850.2169669840218979No Hit
GACTGCATAATTTGTGGTAGTGGGGGA300360.2166136058528079No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGG296640.2139308164874715No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG295960.21344041413036696No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGA264730.19091796470040562No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG258100.1861365417186367No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATA257570.18575431635206996No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT255790.18447061606435522No Hit
CCCCCCGCGGGGGCGCGCCGGC253090.18252343023467554No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGG252460.18206908687441697No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGC252430.18204745147630944No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGG246370.17767710105858397No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTTA244140.17606886979925596No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCC241870.1744317913424512No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGG240690.1735807990168875No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGC237450.17124417602127193No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATAC235490.16983066334491187No Hit
CGTGATCGTATAGTGGTTAGTACTCTGC235080.16953497957077535No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCG233880.16866956364647326No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG229680.16564060791141602No Hit
CCCTGGTGGTCTAGTGGTTAGGATTC228620.16487615717828255No Hit
TCTACAGTCCGACGATC225920.16292897134860287Illumina DpnII expression Sequencing Primer (100% over 17bp)
CTTCCCACGCAGTTTTATCCGGTAAAGCG225520.1626404993738355No Hit
TCGACTGCATAATTTGTGGTAGTGGGG224000.16154430586971955No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG222270.16029666457885075No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC218100.15728934424190105No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC217900.15714510825451738No Hit
GACTGCATAATTTGTGGTAGTGGGGG215490.15540706460654405No Hit
CGACTGCATAATTTGTGGTAGTGGGG214220.15449116608665772No Hit
TTCTACAGTCCGACGATC213150.15371950355415503Illumina DpnII expression Sequencing Primer (100% over 18bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCG208010.1500126386783945No Hit
TCCTCGCCGCGCGGCGGCGGTGGCGGCGGG204680.14761110948845624No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGG202030.14569998265562253No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGC200580.14465427174709086No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG195040.14065893489656295No Hit
CGCCGCTGGTGTAGTGGTATCATGCA194600.14034161572431889No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATT194480.14025507413188867No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGG194020.1399233313609062No Hit
GAGTTCTACAGTCCGACGATC187270.13505536678670707Illumina DpnII expression Sequencing Primer (100% over 21bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGG186930.1348101656081548No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG185000.13341828832990232No Hit
TCCCTGGTGGTCTAGTGGTTAGGAT184200.13284134438036763No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC181740.13106724173554837No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC180320.13004316622512424No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC176420.12723056447114253No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA176410.12722335267177334No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGG172460.1243746919209457No Hit
TTCCCACGCAGTTTTATCCGGTAAAGCG171870.12394919575816385No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTA166460.12004761229943535No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGA165430.1193047969644094No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGA161800.11668691379339564No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCC161210.1162614176306138No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC161040.11613881704133767No Hit
CTACAGTCCGACGATC161000.11610996984386093Illumina DpnII expression Sequencing Primer (100% over 16bp)
CCCTGGTGGTCTAGTGGTTAGGATT160920.11605227544890748No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGG157600.1136579580583384No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAG155210.11193433800910345No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGA154940.1117396194261355No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCGTTA154810.1116458660343361No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTG153570.11075160291255728No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC151790.10946790262484256No Hit
GGGCGCGCGCCGCGGCTGGACGAGGCGCCG149840.10806160174785169No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAG149390.10773707077623841No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCG142520.10278256460960906No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT142500.10276814101087071No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGG142020.10242197464114988No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCT139850.10085701417803697No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)