FastQCFastQC Report
Tue 5 Sept 2023
SRR12764786_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764786_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26438207
Total Bases800.6 Mbp
Sequences flagged as poor quality0
Sequence length15-44
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCGGCCGAGGTGGGATCCCGAGGCCT4785751.8101643579687534No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC4539771.7171247656847533No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT4389501.6602865693577482No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG4129271.5618570502908915No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT3617991.368470259726766No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTT3242501.2264447433973114No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT3174591.200758432672836No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT3075221.1631726765737178No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG2793301.0565391215826399No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG2685791.0158744880089636No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCT2369180.8961197709057955No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT2366640.8951590400967812No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTC2279950.8623693732332152No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT1972880.7462230702709908No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1924060.7277573702331629No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT1868220.7066364220538859No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT1856360.7021504900086455No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA1737190.6570755724849269No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG1727750.6535049823915821No Hit
CAGAGTTCTACAGTCCGACGATC1657970.6271113619770055Illumina DpnII expression Sequencing Primer (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCG1550500.5864618580223688No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG1485310.5618043613925862No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC1380920.5223198381039985No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCC1284210.48574020167101345No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC1258650.4760723751047111No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT1237090.46791751044236846No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1228270.4645814294441374No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT1166670.4412818161231584No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA1137700.4303241895337305No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT1131590.4280131402254321No Hit
GTTCTACAGTCCGACGATC1116910.42246057003790005Illumina DpnII expression Sequencing Primer (100% over 19bp)
TCGCCGGCCGAGGTGGGATCCCGAGGCCTC1090250.41237667894800883No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG1089700.4121686466862144No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCC1066860.40352963421460464No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG1023750.38722368729467926No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC988110.3737431967303985No Hit
AGAGTTCTACAGTCCGACGATC964760.3649112816160339Illumina DpnII expression Sequencing Primer (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGG958960.3627174868552924No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCT954410.36099649268953826No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC943680.35693797238216646No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTG940590.35576920931135764No Hit
ATTGATCATCGACACTTCGAACGCA932300.3526335957654012No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAA925860.3501977271000261No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC897760.3395691697247094No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT893300.33788221720179434No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC881990.3336043174183484No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC873450.33037414375339447No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG848660.3209975623536044No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAT820930.3105089539544039No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGG820220.31024040321645113No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTC805190.3045554488623226No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG762330.28834406206139473No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTC726940.2749581316161115No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG683200.25841389319631247No Hit
AGTTCTACAGTCCGACGATC676980.2560612374356551No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT667770.252577642651788No Hit
CATTGATCATCGACACTTCGAACGCA660660.2498883528674997No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTT652070.2466392671787463No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGG644120.2436322553946264No Hit
CGACTGCATAATTTGTGGTAGTGGGGG599360.22670221168931767No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG578130.21867216638405168No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATAC563350.2130817721489207No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG558810.21136456038792648No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC555730.2101995797218775No Hit
ATTGATCATCGACACTTCGAACGCAC533770.2018934188691389No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATA510440.1930690685642941No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCG506320.19151071780321563No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG506200.19146532894609683No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGG494410.1870058737341757No Hit
CTTCCCACGCAGTTTTATCCGGTAAAGCG493090.186506596305869No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATT488820.18489150947339206No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGA487350.18433549597368687No Hit
TCTACAGTCCGACGATC485140.18349958452174917Illumina DpnII expression Sequencing Primer (100% over 17bp)
TTCTACAGTCCGACGATC477280.18052661438046838Illumina DpnII expression Sequencing Primer (100% over 18bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG457930.17320766117006345No Hit
GAGTTCTACAGTCCGACGATC450840.17052593619529494Illumina DpnII expression Sequencing Primer (100% over 21bp)
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCG447350.16920587693409012No Hit
AGGGCACCACCAGGAGTGGAGCCTGCGG442960.16754540124449438No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTTA442200.16725793848274204No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG422440.15978390667718126No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCC421020.15924680520127554No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCC401390.15182194465759347No Hit
CCCCCCGCGGGGGCGCGCCGGC400270.15139831532448475No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC398760.15082717220574No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGG394370.14916669651614423No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCGTTA392920.14861824782595887No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCC392160.14833078506420652No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGG390210.14759321613602616No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG379460.14352713101913453No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGG378110.14301650637654817No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGG373980.1414543732107098No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGG372820.1410156142585615No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCG362110.13696465876070946No Hit
ACGATGCCGACTGGCGATGCGGCGGCG343080.1297667425026213No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGG338180.12791336417027072No Hit
TTCCCACGCAGTTTTATCCGGTAAAGCG335420.1268694204565385No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGG333530.12615454595691758No Hit
CTACAGTCCGACGATC333370.1260940274807592Illumina DpnII expression Sequencing Primer (100% over 16bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCG331430.12536024095733875No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT329980.12481179226715337No Hit
GAGCCTCGGTTGGCCTCGGATAGCCGGTCCCC328630.12430116762456697No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG322850.12211493767334525No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCC322310.12191068781631069No Hit
TCCTCGCCGCGCGGCGGCGGTGGCGGCGGG321290.12152488253080099No Hit
CGCTTCTGGCGCCAAGCGCCCGGCCG320040.12105208193581357No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG318060.12030316579335354No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGC317240.11999300860304181No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCT308940.11685361265232547No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA308380.11664179798577111No Hit
AGGGCAGGGCGCCCTGGAATGGGTT307690.11638081205733808No Hit
GACTGCATAATTTGTGGTAGTGGGGGA306230.1158285809623928No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGA305890.11569997920055622No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG303380.11475059560582153No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGA302140.11428157741559403No Hit
CGATGCCGACTGGCGATGCGGCGGCG300650.11371799910636904No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCT298930.11306742548766639No Hit
GGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCC296230.11204617620249362No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCC293220.11090767236976397No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGC292030.11045756620333595No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC288380.10907698846597275No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGG282590.10688697610999112No Hit
GGACCGGGGTCCGGTGCGGAGTGCCCTTCG278570.10536644939651163No Hit
AAGGGCAGGGCGCCCTGGAATGGGTT277700.10503738018240041No Hit
CGTGATCGTATAGTGGTTAGTACTCTGC276920.10474235261112828No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG276680.10465157489689071No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCC275220.10409934380194542No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGG275100.10405395494482662No Hit
TCCTCGCCGCGCGGCGGCGGTGGCGGCGGGC272210.10296083996921576No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTA270810.10243130330282989No Hit
CGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT267170.10105450797022657No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGG265890.10057036016095948No Hit
GTGGGGGGCCGGGCCACCCCTCCCACGGCGC264900.10019590208972945No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)