FastQCFastQC Report
Tue 5 Sept 2023
SRR12764787_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764787_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13571409
Total Bases399 Mbp
Sequences flagged as poor quality0
Sequence length15-44
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC9273976.8334614335180675No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG7375425.4345278371611965No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT3893782.8691051901832743No Hit
GCCGGGCGCGGTGGCGCGTGCCT2808852.069681932067628No Hit
CAGAGTTCTACAGTCCGACGATC2064601.5212864043814465Illumina DpnII expression Sequencing Primer (100% over 23bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1927491.4202578376349868No Hit
AGAGTTCTACAGTCCGACGATC1496421.1026268532618833Illumina DpnII expression Sequencing Primer (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC1359601.0018119710341056No Hit
GTTCTACAGTCCGACGATC1335170.9838108924430764Illumina DpnII expression Sequencing Primer (100% over 19bp)
CGCCGGCCGAGGTGGGATCCCGAGGCCTC1275190.9396150392343197No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT1258290.927162389697341No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT1177650.8677433566404196No Hit
CCCCCCGCGGGGGCGCGCCGGC1095640.8073148484435183No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCT1074840.791988510551852No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA1028130.7575705661807112No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG1009860.7441084415037524No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA987260.7274557859099229No Hit
AGTTCTACAGTCCGACGATC972510.7165873491838615No Hit
CGACTGCATAATTTGTGGTAGTGGGGG861740.6349672314790601No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCT837570.6171577321116769No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG799030.5887597964220223No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT686510.5058502031734509No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGG679220.500478616479689No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTC676260.49829756070279807No Hit
TTCTACAGTCCGACGATC611590.45064591303673773Illumina DpnII expression Sequencing Primer (100% over 18bp)
TCTACAGTCCGACGATC611190.45035117576959033Illumina DpnII expression Sequencing Primer (100% over 17bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC598240.4408090567456923No Hit
GAGTTCTACAGTCCGACGATC597070.43994695023928615Illumina DpnII expression Sequencing Primer (100% over 21bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG576210.42457640175754785No Hit
CCCCCCGCGGGGGCGCGCCGG543090.4001721560377408No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG541060.3986763644069676No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG528440.3893774036284663No Hit
CCCCCGCGGGGGCGCGCCGGC522830.3852437134567236No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG508590.37475106674627523No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG508490.37467738242948834No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGG446130.32872784248120446No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGG417680.30776465435534367No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCC413590.3047509657987612No Hit
CTACAGTCCGACGATC390060.2874130460588138Illumina DpnII expression Sequencing Primer (100% over 16bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC388870.28653620268905017No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGG383180.28234356506387803No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG378450.27885829687985975No Hit
CGACTGCATAATTTGTGGTAGTGGGG371330.2736119735246355No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG359020.26454143412817344No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC354390.26112985026094193No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG350520.2582782672012906No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGG346790.25552984218514085No Hit
TCGACTGCATAATTTGTGGTAGTGGGG326820.2408150841228055No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG325090.2395403454423929No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG322200.23741086868725275No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT314850.23199507140341877No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGG312600.23033717427571446No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG307970.226925590408483No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT306490.22583506252003752No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC296860.21873926281346323No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG295870.2180097880772733No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG285720.21053082992340735No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTC282130.2078855629507592No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC281620.20750977293514622No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC281480.20740661489164464No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGG279850.20620556052801886No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT266650.19647923071215379No Hit
TAAGTGGGAGGCCCCCGGCGCCCCCCCGTC264210.19468133338255447No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGG262700.1935687001990729No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCG252270.18588342595820376No Hit
AAATTCTAGAATTAAAGCATCTATG251740.18549289907923341No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCT251290.18516131965369256No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGC247940.18269289504133285No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGG241780.17815394112726246No Hit
CCCCCGCGGGGGCGCGCCGG234930.17310656542736277No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGC229980.16945919174641336No Hit
AGGGCACCACCAGGAGTGGAGCCTGCGG229000.16873708544190216No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTG226630.16699076713405364No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG223090.16438234231979892No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGG215620.1588781238558207No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCG214260.15787601714751948No Hit
CGTGATCGTATAGTGGTTAGTACTCTGC212300.15643180453849706No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCG210760.15529706605997948No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG210040.1547665389791141No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCG207170.1526517990873313No Hit
TAAGTGGGAGGCCCCCGGCGCCCCCCCGTCCCC203170.14970442641585704No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC199150.1467423168810254No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGG195560.14409704990837724No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC195540.14408231304501987No Hit
TTGGCAATTTTTGACAGTCTCCACGGAGACTG189970.13997809659999194No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG186800.13764230375784858No Hit
TACAGTCCGACGATC185480.13666967077626205Illumina DpnII expression Sequencing Primer (100% over 15bp)
CAGGCGGCCCGGGTTCGACTCCCGGTGTGG184380.13585914329160664No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGG183160.134960194626807No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATG182780.1346801942230169No Hit
GCCGCTGGTGTAGTGGTATCATGCAAGATT178200.13130545251417888No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG177900.13108439956381832No Hit
CGGGAGACCGGGGTTCAATTCCCCGACGGG176000.12968439754486805No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTG175890.12960334479640248No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC174040.12824018493584566No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCC172600.12717913077411488No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTC169910.12519702265254845No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGT167270.12325175668937544No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCC167140.12315596707755253No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGG165690.1220875444841431No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTC163570.12052543696826175No Hit
TCCTTAGGTCGCTGGTTCGTTTCCGGCTCGAA162520.11975175164199975No Hit
GGGAGACCGGGGTTCGATTCCCCGACGG162190.11950859339660311No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCC158400.11671595779038123No Hit
GCTGGTCCGATGGTAGTGGGTTATCAGAA156430.11526437674968017No Hit
GACTGCATAATTTGTGGTAGTGGGGG155360.11447595456006078No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGG153330.1129801629292876No Hit
CCCCGGGGAGCCCGGCGGGCGCCGGCGCGCCCCGCT153310.11296542606593021No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAG152920.11267805723046147No Hit
CGCCGGGCGCGGTGGCGCGTGCCT151940.11195595092595029No Hit
TAAGTGGGAGGCCCCCGGCGCCCCCCCGTCC151220.11142542384508491No Hit
TCCTCGCCGCGCGGCGGCGGTGGCGGCGGG151180.11139595011837017No Hit
GTGGGGGGCCGGGCCACCCCTCCCACGGCG149920.11046752772685578No Hit
TCCTCGCCGCGCGGCGGCGGTGGCGGCGGGC148420.10936226297505293No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCG147010.10832331410835824No Hit
CGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT146710.10810226115799766No Hit
TTGCGACCCGGGTTCGTTTCCCGGGCGGCG143600.10581067890592642No Hit
AGACCGGGGTTCGATTCCCCGACGGGG143420.10567804713571007No Hit
TCTACAGTCCGACGATCCAGAGT142010.1046390982690154No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGG141950.10459488767894329No Hit
CGTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCG141500.10426330825340244No Hit
AGGTCGCTGGTTCGTTTCCGGCTCGAAGG139210.10257593739898341No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCC137760.10150751480557398No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGG137610.10139698833039369No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGC136550.10061593457245302No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)