FastQCFastQC Report
Tue 5 Sept 2023
SRR12764788_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764788_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5224843
Total Bases140.7 Mbp
Sequences flagged as poor quality0
Sequence length15-44
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC3628056.943845011228089No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG3310706.336458339513743No Hit
CAGAGTTCTACAGTCCGACGATC3045705.8292660659851405Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGAGTTCTACAGTCCGACGATC2312464.425893754128114Illumina DpnII expression Sequencing Primer (100% over 22bp)
GTTCTACAGTCCGACGATC1962273.755653519158374Illumina DpnII expression Sequencing Primer (100% over 19bp)
AGTTCTACAGTCCGACGATC1484062.840391567746629No Hit
TTCTACAGTCCGACGATC927741.7756323012959434Illumina DpnII expression Sequencing Primer (100% over 18bp)
TCTACAGTCCGACGATC888541.7006061234758632Illumina DpnII expression Sequencing Primer (100% over 17bp)
GAGTTCTACAGTCCGACGATC871911.668777415895559Illumina DpnII expression Sequencing Primer (100% over 21bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG758551.4518139588117767No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT690701.3219535974573782No Hit
CTACAGTCCGACGATC556791.0656588150112836Illumina DpnII expression Sequencing Primer (100% over 16bp)
CGACTGCATAATTTGTGGTAGTGGGG522771.000546810688857No Hit
CGACTGCATAATTTGTGGTAGTGGGGG429280.8216132044541817No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCG403920.7730758608440483No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCT373900.7156195889522422No Hit
ATACCACCCTGAACGCGCC338100.6471007836981896No Hit
ACCAGGGGTCGCGAGTTC324990.6220091206568312No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG280520.5368965153594089No Hit
TACAGTCCGACGATC268240.5133934167974042Illumina DpnII expression Sequencing Primer (100% over 15bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGC264950.507096576873219No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT245960.47075098715884856No Hit
CTGGTCCGATGGTAGTGGGTTATCAGAA245240.4693729553213369No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGC240270.45986070777629107No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG214280.410117586308335No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA207690.39750476712888794No Hit
ATCGCATGATGAGTAATAGAAAGGAGCTT180950.3463261958301905No Hit
CAGAGTTCTACAGTCCGA170750.32680407813210843Illumina DpnII expression Adapter 1 (100% over 18bp)
CCAGGGGTCGCGAGTTC170660.3266318241524195No Hit
CAAGCGGTGGAGCATGTGGTTTAATTTG159580.305425445319601No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTG157490.30142532512460185No Hit
CCCCACAACCGCGTTTGACTAGCTTGACATTTT156620.2997602033209419No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT145680.27882177512319506No Hit
CCCCACAACCGCGTTTGACTAGCTTGACATTT138450.26498403875484866No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTC132570.2537301120818367No Hit
GACTGCATAATTTGTGGTAGTGGGG129830.24848593536686173No Hit
AGAGTTCTACAGTCCGA117510.22490627948055092Illumina DpnII expression Adapter 1 (100% over 17bp)
TCGACTGCATAATTTGTGGTAGTGGGG116370.22272439573782407No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG105120.2011926482767042No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCT100780.19288617858948107No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAG98850.18919228769170673No Hit
TACCACCCTGAACGCGCC94980.18178536656508149No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTA88060.16854094946010817No Hit
ACAGAGTTCTACAGTCCGACGATC87070.16664615568352964Illumina DpnII expression Sequencing Primer (95% over 24bp)
CGTGGGGGGCCGGGCCACCCCTCCCACGGCG85770.164158042643578No Hit
GACTGCATAATTTGTGGTAGTGGGGG85160.1629905434479084No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG85160.1629905434479084No Hit
CAGAGTTCTACAGTC79870.1528658373084129Illumina DpnII expression Adapter 1 (100% over 15bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC79710.1525596080111881No Hit
CCCCCGCGGGGGCGCGCCGG76930.1472388739719069No Hit
CCCCCGCGGGGGCGCGCCGGC75400.1443105563171946No Hit
CAGAGTTCTACAGTCCGACGAT73070.13985109217635822Illumina DpnII expression Sequencing Primer (100% over 22bp)
CAGAGTTCTACAGTCCACGATC71680.13719072515671762No Hit
AGTTCTACAGTCCGA70540.1350088414139908Illumina DpnII expression Adapter 1 (100% over 15bp)
TCCAGCGATCCGAGTTC66380.12704687968614561No Hit
AGCGGGAAGCTTCCTCAAAGATGAGTATTCCT66360.1270086010239925No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG65730.12580282316616978No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC65490.12534347922033257No Hit
TATCAACCTGTACCGAGAACGAACACACGTTCCCA65420.1252095039027967No Hit
GCTGGTCCGATGGTAGTGGGTTATCAGAA64090.1226639728696154No Hit
TATCAACCTGTACCGAGAACGAACACACGTTCCCAA63360.1212668017010272No Hit
TAAACCAGGGGTCGCGAGTTC61720.11812795140447281No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGG60940.11663508358050186No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTT60570.11592692833066946No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG60350.11550586304698535No Hit
TCAGAGTTCTACAGTCCGACGATC58990.11290291402057441Illumina DpnII expression Sequencing Primer (100% over 24bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG58830.11259668472334958No Hit
GTGGGGGGCCGGGCCACCCCTCCCACGGCG58450.11186939014244066No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA57400.10985976037940277No Hit
ATCCAGCGATCCGAGTTC55780.10675918874500152No Hit
TTCAGAGTTCTACAGTCCGACGATC54070.10348636313091131Illumina DpnII expression Sequencing Primer (100% over 25bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC53230.10187865932048101No Hit
CGTGATCGTATAGTGGTTAGTACTCTG52660.10078771744911762No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)