FastQCFastQC Report
Tue 5 Sept 2023
SRR12764797_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764797_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25072235
Total Bases795.1 Mbp
Sequences flagged as poor quality0
Sequence length15-44
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT5507912.196816518351874No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC4733491.8879409833227871No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG4697601.8736263440415262No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG4567101.821576736178486No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT4252281.6960115442440613No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG3980741.5877084751319537No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC2908131.1599005832547438No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG2719611.0847098393900663No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2657951.060116898234242No Hit
CAGAGTTCTACAGTCCGACGATC2650621.0571933455473754Illumina DpnII expression Sequencing Primer (100% over 23bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2629741.048865408289289No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG2299800.9172696410990085No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGG1976760.7884259221405671No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG1897460.7567973098529109No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA1868400.7452067994736009No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGT1798190.7172037115957154No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG1790890.7142921243359438No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGA1769610.7058046480499246No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC1713540.6834412648094595No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC1669950.6660554992404946No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC1633770.6516251941639826No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC1587490.6331665286321702No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC1564860.6241406081268782No Hit
AGAGTTCTACAGTCCGACGATC1370390.546576721221702Illumina DpnII expression Sequencing Primer (100% over 22bp)
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT1319020.5260879215594461No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG1238930.49414421969162303No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGG1209010.4822107004022577No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGA1181830.4713700234542313No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA1156480.461259237558997No Hit
GTTCTACAGTCCGACGATC1078590.4301930003448037Illumina DpnII expression Sequencing Primer (100% over 19bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGA999800.3987678003177618No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC986120.39331156556246377No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGA945300.37703060776193265No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG910090.36298718482815756No Hit
AGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGTG902870.36010750537397246No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA895690.35724377982258065No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA894590.35680504749576575No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGG890640.3552295995949304No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC862560.3440299598340555No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG846130.3374768942617202No Hit
AGTTCTACAGTCCGACGATC826770.3297552053097779No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGG812840.3241992586620219No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT800190.3191538369036506No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCC795810.3174068845477876No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA786930.31386511812768186No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGA785640.31335060476259896No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGC739820.29507540911290914No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGT721250.28766880974113396No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGG707250.2820849437634898No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGA699130.2788463014964561No Hit
TAACCCAGAGGTCGATGGATCGTAACCATCCTCTGC678870.27076564973166534No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTC672630.2682768408959153No Hit
CGACTGCATAATTTGTGGTAGTGGGGG665630.2654849079070932No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGT663590.2646712588646365No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGG645830.257587726024425No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC644070.25688575430152116No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGG641550.25588065842554525No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT636750.25396619009035293No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG635730.25355936556912456No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGG624940.24925580029064023No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC615910.24565420673505972No Hit
TAACCCAGAGGTCGATGGATCGAAACCATCCTCTGC612760.24439783689008976No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC592740.2364129085420586No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGT585330.23345744804960547No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG583660.23279137260798646No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCG575230.22942908759430503No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG572560.2283641645828543No Hit
CCCCCGCGGGGGCGCGCCGGC569070.2269721865641416No Hit
TCAGAAGGTTGCGTGTTCAGGTCACGTCGGGG564800.22526910744096007No Hit
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGG557780.22246919750074137No Hit
TCTACAGTCCGACGATC552520.2203712592834265Illumina DpnII expression Sequencing Primer (100% over 17bp)
TAATCTCAGGGTCGTGGGTTCGTGCCCCACGTTGGG532010.21219089562617774No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGG528880.2109425027326044No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCT514800.20532672894937368No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGG511740.20410625538568858No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGGC509240.20310913646110926No Hit
GAGTTCTACAGTCCGACGATC504280.20113085251474389Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCGCCGCTGGTGTAGTGGTATCATGCAAG498480.19881753660971985No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG498380.19877765185273671No Hit
TTCTACAGTCCGACGATC498080.19865799758178718Illumina DpnII expression Sequencing Primer (100% over 18bp)
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGC491780.19614525789184728No Hit
TCCTTAGGTCGCTGGTTCGTTTCCGGCTCGAAGG489190.19511224268598312No Hit
TAACCCAGAGGTCGATGGATCGTAACCATCCTCTG469660.18732274964716947No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG463430.1848379292871178No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGT454380.18122835878014065No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGG447160.17834867932595558No Hit
CGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATG443940.17706439015109743No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCG442600.1765299344075229No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGG438070.17472315491618517No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCC431120.17195116430585466No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGG429120.1711534691661912No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGC419630.1673684057284881No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC419060.16714106261368403No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC417690.16659464144301456No Hit
TAATCCCAGGGTCGTGGGTTCGTGCCCCACGTTGGG412290.16444086456592322No Hit
CGACAGAGTGGTTCATTTCCACCTTTCGGGCGCCA406880.16228309921313355No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT406540.16214749103939077No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC403700.16101476394106867No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCT402730.1606278817983319No Hit
CTACAGTCCGACGATC381580.15219225569639086Illumina DpnII expression Sequencing Primer (100% over 16bp)
AGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCG379120.15121109067460478No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAG376880.15031767211818173No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGA372890.14872627031455313No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCC372410.1485348234810339No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG364810.14550358195031277No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG339770.13551643880172629No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC338790.1351255681832912No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACG331180.13209033817687177No Hit
TCCTTAGGTCGCTGGTTCGTTTCCGGCTCGAAG326100.13006419252212656No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCC323480.12901921188916743No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGG321380.1281816319925208No Hit
CCCGCGAGGGGGGCCCGGGC320760.12793434649922514No Hit
CGCGGGAGACCGGGGTTCAATTCCCCGACGGGG318860.12717653611654484No Hit
TAATCTCAGGGTCGTGGGTTCGGGCCCCACGTTGGG315310.12576062724364223No Hit
TAACCCAGAGGTCGATGGATCGAAACCATCCTCTG315260.12574068486515064No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGA314430.1254096413821903No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTC310650.12390199756822638No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGA308560.12306840614727806No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGC306750.12234649204588262No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG296620.11830616616348721No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTG288360.11501168523667714No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGC285510.11387496966265673No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCCCC281150.11213599425819037No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGC279510.11148188424366634No Hit
CGCAGCAACCTCGGTTCGAATCCGAGTCACGG278250.11097933630567838No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCG275410.10984660920735627No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGG273970.10927226870679858No Hit
AGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGT271710.10837087319897887No Hit
TTCTTGCGACCCGGGTTCGATTCCCGGGCGGCG271480.10827913825791756No Hit
TCCTCGTTAGTATAGTGGTGAGTATCCCCG270070.10771676318445485No Hit
TTCTTGCGACCCGGGTTCGGTTCCCGGGCGGCG265750.10599374168278176No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGG265460.10587807588753058No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC264790.10561084801574332No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAG263510.10510032312635871No Hit
CGCGGGAGACCGGGGTTCAATTCCCCGACGGGGA263370.10504448446658225No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGG251520.10031814076407629No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)