FastQCFastQC Report
Tue 5 Sept 2023
SRR12764798_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764798_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12311716
Total Bases373.9 Mbp
Sequences flagged as poor quality0
Sequence length15-44
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC3571962.901269002631315No Hit
CAGAGTTCTACAGTCCGACGATC2679682.176528438440263Illumina DpnII expression Sequencing Primer (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCG2313351.8789825886172162No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC2205871.7916836288296447No Hit
GTTCTACAGTCCGACGATC2111681.7151792650187836Illumina DpnII expression Sequencing Primer (100% over 19bp)
AGAGTTCTACAGTCCGACGATC1952011.5854897887508126Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT1864571.5144680075466328No Hit
AGTTCTACAGTCCGACGATC1612291.3095574979149942No Hit
TCTACAGTCCGACGATC1533371.2454559543121364Illumina DpnII expression Sequencing Primer (100% over 17bp)
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG1295491.0522416209080847No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT1277451.0375889112451913No Hit
TTCTACAGTCCGACGATC1207670.9809111906090101Illumina DpnII expression Sequencing Primer (100% over 18bp)
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG1194220.9699866371186601No Hit
CTACAGTCCGACGATC1139930.9258904282717372Illumina DpnII expression Sequencing Primer (100% over 16bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1106050.8983719247584984No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1095740.8899977874733302No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT1020110.8285684952446921No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC992530.8061670688310225No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG956840.7771784209447327No Hit
GAGTTCTACAGTCCGACGATC830900.6748856130209632Illumina DpnII expression Sequencing Primer (100% over 21bp)
CGCCGGCCGAGGTGGGATCCCGAGGCCTC781560.6348099647522734No Hit
CCCCCGCGGGGGCGCGCCGGC773640.6283770678271007No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC684870.5562750147907896No Hit
AGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGTG684560.5560232221081123No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTC680350.5526037150304637No Hit
TACAGTCCGACGATC679810.5521651084219292Illumina DpnII expression Sequencing Primer (100% over 15bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC674870.5481526701882987No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG652530.5300073523463342No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC649050.5271807764246673No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGG599640.4870482717437602No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT596870.4847983822888702No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCT596540.48453034491698804No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCC585470.47553890944203064No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT571130.4638914672820588No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC567940.4613004393538642No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC554600.4504652316541415No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC540630.43911831624446174No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG531740.4318975518928474No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA498980.4052887509750875No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCC480640.3903923709741193No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG443650.3603478182895057No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC442360.35930003583578435No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG418150.33963583955315413No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCG404730.3287356530966114No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG404080.3282077006974495No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGA390550.3172181684502794No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGT383940.31184929866803296No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA367920.29883730261484265No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT367340.29836620662789815No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA366380.29758646154605906No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGA364710.2962300299974431No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCT364160.2957833010443061No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC359880.29230693755444004No Hit
CCCGCGAGGGGGGCCCGGGC323470.2627334808567709No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT308990.2509723258723642No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGG271680.2206678581604709No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC265430.21559139278391412No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA265390.21555890340550415No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC262510.2132196681599868No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG255400.20744468114761583No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGC251590.20435006785406684No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGA250800.20370840263047005No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA240790.19557793568337672No Hit
CGACAGAGTGGTTCATTTCCACCTTTCGGGCGCCA234760.19068016188807474No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG231780.18825970319653246No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGG221650.18003176811420926No Hit
TAACCCAGAGGTCGATGGATCGTAACCATCCTCTGC221390.1798205871545445No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGG221270.1797231190193146No Hit
TCTACAGTCCGACGATCCAGAGT220460.17906520910651286No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG215530.17506089321748486No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGC214260.17402935545296855No Hit
CGACTGCATAATTTGTGGTAGTGGGGG210600.17105657732845692No Hit
AGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGT200400.16277178583391624No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG199990.16243876970521412No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGG199370.16193518433985968No Hit
CTACAGTCCGACGATCCAGAGTT196170.15933603406706262No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGA192810.1566069262806257No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGGC192650.15647696876698586No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGC182980.14862266153637724No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGT181300.14725810764315875No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTC177470.14414724966040476No Hit
TAACCCAGAGGTCGATGGATCGAAACCATCCTCTGC174480.14171866862426No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCG171720.13947690151397255No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC169060.13731635784970997No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTG169010.13727574612669752No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCC168180.13660159152469079No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC167710.13621984132837373No Hit
CCCCCGCGGGGGCGCGCCGG164990.1340105635964962No Hit
TAATCTCAGGGTCGTGGGTTCGTGCCCCACGTTGGG163240.13258915329106033No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGG161820.13143578035750664No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCT161050.13081035982311484No Hit
CGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATG159080.12921025793642413No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC156720.12729338461023632No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGT155790.12653800656220465No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC155310.1261481340212851No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGC155190.1260506658860552No Hit
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGG150130.12194075951719484No Hit
TAACCCAGAGGTCGATGGATCGTAACCATCCTCTG149720.12160774338849271No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCG147040.11943095503502517No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGG146940.11934973158900027No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGC142040.11536978273377976No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG140910.11445195779369828No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG139430.11324985079252964No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGT135680.11020397156659559No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGA133860.10872570484894227No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG133060.10807591728074299No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG130610.10608594285313273No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGA127680.10370609588460293No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC127590.10363299478318051No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGC127580.10362487243857801No Hit
CGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGA126470.10272329218770154No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCG126200.10250398888343429No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCT126080.10240652074820439No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC125660.1020653822748998No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGC125120.10162677566636527No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)