FastQCFastQC Report
Tue 5 Sept 2023
SRR12764799_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764799_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12787860
Total Bases378.8 Mbp
Sequences flagged as poor quality0
Sequence length15-44
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAGTTCTACAGTCCGACGATC4099703.2059312504203206Illumina DpnII expression Sequencing Primer (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC3673572.872701139987457No Hit
GTTCTACAGTCCGACGATC3581702.800859565243911Illumina DpnII expression Sequencing Primer (100% over 19bp)
AGAGTTCTACAGTCCGACGATC3161502.47226666541548Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTTCTACAGTCCGACGATC2670982.088684111336846No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2149571.6809458345649704No Hit
TCTACAGTCCGACGATC1995571.560519117350362Illumina DpnII expression Sequencing Primer (100% over 17bp)
TTCTACAGTCCGACGATC1843081.441273207557793Illumina DpnII expression Sequencing Primer (100% over 18bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCG1838381.4375978467077368No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC1792381.4016262298773994No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT1463151.144171112289312No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT1423881.1134622993995869No Hit
GAGTTCTACAGTCCGACGATC1350541.0561110303053052Illumina DpnII expression Sequencing Primer (100% over 21bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1239080.9689502387420569No Hit
CTACAGTCCGACGATC1217520.9520904983320119Illumina DpnII expression Sequencing Primer (100% over 16bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG1162460.9090340369694382No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG1115960.8726714243039884No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG1068300.8354017013010778No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG1014270.7931506913588355No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC993700.777065122702313No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC967110.7562719641910375No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT846960.6623156650135363No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA686530.536860741359383No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTC666970.5215649842897874No Hit
CCCCCGCGGGGGCGCGCCGGC652240.5100462469873771No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG638080.49897324493699496No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC634080.495845278256096No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGG612290.47880567976189914No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA591820.4627983102723989No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG588890.46050707467864055No Hit
TACAGTCCGACGATC563630.4407539650887639Illumina DpnII expression Sequencing Primer (100% over 15bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC549980.4300797787901964No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTC542870.42451981801489846No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC538640.42121199324984787No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGG527200.41226600854247697No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGT500480.3913711911140722No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT476950.3729709271136844No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGA458520.3585588206314426No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG458040.35818346462973477No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC431200.33719480820090303No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCT418220.32704455632138607No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA403500.31553363893567804No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG387610.3031077912958071No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT381460.29829854252392507No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC381390.2982438031070093No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCC369640.28905540098186877No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGA364910.2853565803817058No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC364830.2852940210480878No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC354380.2771222080942394No Hit
CGACTGCATAATTTGTGGTAGTGGGGG351900.2751828687520821No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG341090.2667295387969527No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGC340980.266643519713228No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA300190.2347460794847613No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC283340.22156951984147466No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGG273940.21421879814136222No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG273490.21386690188976107No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT268790.21019154103970486No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG265040.20725907227636212No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGA256370.20047920449551374No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGA244010.19081378745153607No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCC238370.1864033544314686No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT236090.18462041342335622No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA235780.18437799600558655No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGG231310.180882493239682No Hit
CGTCGCGCGCGCGTCCGCTGGGGGCGGGGAGCGGT228250.17848959872879433No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCCCC223940.17511921463012575No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG219980.17202252761603584No Hit
CGCCGGCCGAGGTGGGATCCCGAGGC212350.16605593117222114No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGT211050.165039342000929No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC210930.16494550300050204No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGG210340.16448412791506944No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCT207350.1621459728210975No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC205620.16079312723160874No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGG205050.16034739197958064No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG204860.16019881356223795No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGA202640.15846279205433902No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGG199170.15574928095865923No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCG199000.15561634237472102No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC198210.1549985689552435No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG196700.15381776153320414No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGT194950.1524492761103109No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG194060.15175330352381086No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC192000.15014240068314794No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG188510.1474132497540636No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC188510.1474132497540636No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGT187450.1465843385836254No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCC184740.1444651411573164No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGGC183120.14319831465155233No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCG174560.13650446595442867No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG173240.135472236949732No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGG169910.13286820468788366No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGA166750.13039711100997353No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC164450.12859853016845665No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCC164070.12830137333377126No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC156500.12238169639017006No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGG156420.12231913705655208No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGC154620.12091155205014757No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC153640.12014520021332734No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGA153490.12002790146279363No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC152180.11900349237479921No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC149690.11705633311593965No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGC148820.11637600036284414No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCG148270.11594590494422054No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGC144260.11281011834661937No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCG142200.11119921550595643No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGC142050.11108191675542273No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCG140820.1101200670010463No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGC138610.10839186540984966No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTG137140.1072423376546193No Hit
GACTGCATAATTTGTGGTAGTGGGGGA137050.10717195840429906No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCG134290.10501366139447883No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCT134200.1049432821441586No Hit
TAACCCAGAGGTCGATGGATCGTAACCATCCTCTGC133740.10458356597585522No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGA133000.10400489213988892No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG132570.1036686357216923No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG130400.10197171379730463No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)