FastQCFastQC Report
Tue 5 Sept 2023
SRR12764800_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764800_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14759739
Total Bases444.8 Mbp
Sequences flagged as poor quality0
Sequence length15-44
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC5320193.604528508261562No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC3014762.0425564435793886No Hit
CAGAGTTCTACAGTCCGACGATC2984452.022020850097688Illumina DpnII expression Sequencing Primer (100% over 23bp)
GTTCTACAGTCCGACGATC2496591.691486549999292Illumina DpnII expression Sequencing Primer (100% over 19bp)
AGAGTTCTACAGTCCGACGATC2320641.5722771249545808Illumina DpnII expression Sequencing Primer (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCG2287811.550034184208813No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG1904871.2905851519461151No Hit
AGTTCTACAGTCCGACGATC1804731.2227384237621004No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT1620421.097864941920721No Hit
TCTACAGTCCGACGATC1612321.092377040000504Illumina DpnII expression Sequencing Primer (100% over 17bp)
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG1514591.026163132017443No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTC1350050.9146841959739261No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT1323740.896858677514555No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT1322370.8959304768194072No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1308410.8864723149914778No Hit
TTCTACAGTCCGACGATC1301500.881790660390404Illumina DpnII expression Sequencing Primer (100% over 18bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC1151350.7800612192397168No Hit
CTACAGTCCGACGATC1148490.7781235155987515Illumina DpnII expression Sequencing Primer (100% over 16bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC1146570.7768226795880333No Hit
CCGCCGAGGGCGCACCACCGGCCCGTC1140940.7730082489941049No Hit
CCCCCGCGGGGGCGCGCCGGC1106050.7493696196118373No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC1091550.7395455976558934No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1030430.6981356513147015No Hit
GAGTTCTACAGTCCGACGATC972910.6591647724936057Illumina DpnII expression Sequencing Primer (100% over 21bp)
TCGCCGGCCGAGGTGGGATCCCGAGGCCTC848210.574678183672489No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG776510.5261000888972359No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC768350.520571535851684No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG737550.4997039581797483No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT716150.4852050568102864No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG702280.4758078716703595No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG693880.47011671412346795No Hit
AGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGTG675730.45781974870964853No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGT668920.45320584598413294No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA659410.4467626426185449No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC644930.43695217103771283No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGG634660.4299940534178822No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC631860.4280970009022517No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCT627600.42521077100347093No Hit
TACAGTCCGACGATC612990.4153122219844131Illumina DpnII expression Sequencing Primer (100% over 15bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCC612570.4150276641070686No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC580400.39323188573998497No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA579930.39291345192486127No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCC513170.34768229980218485No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT512790.34742484267506357No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC483370.3274922408858314No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT473340.3206967277673406No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG464770.31489039203200003No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC451940.30619782639787874No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGA444780.3013467921079092No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA432890.2932910941040353No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGG424510.2876134869322554No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGA416370.28209848426181516No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG406420.275357172643771No Hit
CGACTGCATAATTTGTGGTAGTGGGGG376030.25476737766162394No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG366850.24854775548537816No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT366550.24834449985870347No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCT357660.2423213581215765No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA356290.24139315742642875No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG354900.24045140635616932No Hit
CCCCCGCGGGGGCGCGCCGG347780.2356274728164231No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCG341120.23111519790424478No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA307350.20823538952822948No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC302690.2050781521272158No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGG296600.20095206290571943No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG291610.19757124431536358No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG288010.19513217679526718No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC283680.19219852058359568No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC261270.17701532527099564No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC258530.17515892388070006No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC256300.17364805705575145No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGT253500.17175100454012093No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGC252770.1712564158485458No Hit
TAACCCAGAGGTCGATGGATCGTAACCATCCTCTGC249650.16914255733112896No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGG245110.16606662218078516No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGG240330.16282808252910164No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG232470.1575027851102245No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGA225340.1526720763829225No Hit
CCCGCGAGGGGGGCCCGGGC222750.15091730280596424No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG216350.14658118277023732No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC215750.14617467151688793No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGA215560.1460459429533273No Hit
TAATCTCAGGGTCGTGGGTTCGTGCCCCACGTTGGG210820.14283450405186704No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTC209280.14179112516827025No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCC208540.1412897612891393No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGG208150.14102552897446222No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCG207800.1407883974100084No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGA207500.1405851417833337No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGT200340.1357341074933642No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGT200280.13569345636802926No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGC199530.13518531730134253No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG197980.13413516323018992No Hit
TAACCCAGAGGTCGATGGATCGAAACCATCCTCTGC190280.12891826881220597No Hit
CGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGA189160.12815944780595376No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGG188250.12754290573837382No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCG188030.1273938516121457No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG187820.12725157267347342No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGC185640.12577458178630394No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGG184470.12498188484227261No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGGC184280.12485315627871199No Hit
TAATCCCAGGGTCGTGGGTTCGTGCCCCACGTTGGG184050.12469732696492802No Hit
CGTCGCGCGCGCGTCCGCTGGGGGCGGGGAGCGGT182100.12337616539154249No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC176660.11969046336117461No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGA174500.11822702284911679No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTC174170.11800344165977461No Hit
CCGCGAGGGGGGCCCGGGC166840.11303722918135613No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGA166530.1128271983671256No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC164280.11130278116706536No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGG164000.1111130759155023No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTG163870.11102499847727659No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG161720.10956833315277459No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCC161450.10938540308876735No Hit
TTGCGACCCGGGTTCGTTTCCCGGGCGGCG160870.10899244221052959No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCC155310.10522543792949185No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCT149860.10153296071156814No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)