FastQCFastQC Report
Tue 5 Sept 2023
SRR12764801_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764801_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15027944
Total Bases446.1 Mbp
Sequences flagged as poor quality0
Sequence length15-44
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAGTTCTACAGTCCGACGATC3978282.6472550070721583Illumina DpnII expression Sequencing Primer (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC3662282.4369800685975407No Hit
GTTCTACAGTCCGACGATC3497832.3275505950780757Illumina DpnII expression Sequencing Primer (100% over 19bp)
AGAGTTCTACAGTCCGACGATC3207902.134623339027614Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTTCTACAGTCCGACGATC2641421.7576722404608374No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2555301.7003656654562993No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG2429171.6164353553619843No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC2238011.4892323261252505No Hit
TCTACAGTCCGACGATC2180351.4508638041238375Illumina DpnII expression Sequencing Primer (100% over 17bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2147701.429137611904862No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT1953761.3000846955511678No Hit
TTCTACAGTCCGACGATC1853341.2332625141536329Illumina DpnII expression Sequencing Primer (100% over 18bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATT1700851.13179154779922No Hit
CTACAGTCCGACGATC1398840.9308259333412474Illumina DpnII expression Sequencing Primer (100% over 16bp)
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC1386190.9224082815320578No Hit
GAGTTCTACAGTCCGACGATC1361750.9061452451512995Illumina DpnII expression Sequencing Primer (100% over 21bp)
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG1224640.814908546372012No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG1212130.8065840543456909No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT1168120.7772986111739569No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA1106530.7363149609820212No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG976660.6498959538310762No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC904920.6021582193811742No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC845860.5628580995510764No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT773120.5144549380806849No Hit
CCCCCGCGGGGGCGCGCCGGC745050.4957764016155503No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA742700.4942126481174005No Hit
TACAGTCCGACGATC696380.4633900685283363Illumina DpnII expression Sequencing Primer (100% over 15bp)
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG687950.4577805187456115No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC685340.4560437542221345No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG660810.439720829409532No Hit
AGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGTG654900.43578815571844026No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC598150.3980251723056727No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG587660.39104484286074No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTC577970.38459685503219865No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT568810.3785015435245167No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGT551730.3671360500145595No Hit
CAGAGTTCTACAGTCCGACGA545290.36285070000260844Illumina DpnII expression Sequencing Primer (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCC543730.36181263385064516No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGG541090.3600559065165534No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG517810.3445647654795626No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCT512020.34071194303092955No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA508030.33805688921917726No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC494330.3289405390384739No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG492910.3279956326693791No Hit
GTTCTACAGTCCGACGA488240.3248880884836941Illumina DpnII expression Sequencing Primer (100% over 17bp)
CTCGCCGGCCGAGGTGGGATCCCGAGGCC481300.32027002496149837No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC461550.3071278413068348No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG447130.29753238367137913No Hit
AGAGTTCTACAGTCCGACGA431530.2871517221517461No Hit
CACTGCACTCCAGCCTGGGCAA430680.2865861091843302No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGG426140.2835650705113088No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC421700.28061057454033633No Hit
CGACTGCATAATTTGTGGTAGTGGGGG415730.2766379752280152No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG395890.2634359031415076No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT387100.2575867996314067No Hit
AGTTCTACAGTCCGACGA353490.2352217974727614Illumina DpnII expression Sequencing Primer (100% over 18bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCG340540.22660451755742503No Hit
CCGCCGAGGGCGCACCACCGGCCCGTC335830.22347035629092044No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC318590.21199839445768498No Hit
CACTGCACTCCAGCCTGAGCAA315150.20970932550720178No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC315150.20970932550720178No Hit
TCTACAGTCCGACGA304500.20262252773899078Illumina DpnII expression Sequencing Primer (100% over 15bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGG303340.2018506323952232No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG295960.19693978098401219No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA281960.18762380269716203No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGG276560.18403049678651984No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC274160.18243347193734552No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC265840.17689711912687456No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGA263790.17553299373487152No Hit
TTCTACAGTCCGACGA252540.16804693975436694Illumina DpnII expression Sequencing Primer (100% over 16bp)
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG251520.16736820419346785No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGG245450.16332906217909782No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG243310.1619050483552507No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC241590.16076051388000914No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA240050.15973575626845563No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGA233810.1555834916606024No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGT232200.15451215415761463No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGG229670.15282862379577672No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGT224050.14908892394062687No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGG219560.14610115661862993No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGA219310.1459347998635076No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG217820.14494331360297855No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGT215420.14334628875380426No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTG211280.14059142088897855No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC209650.139506774845581No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTC203760.13558740969489905No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGC193710.12889986813898163No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGA188890.1256925099002232No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG188390.12535979638997857No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG185260.12327700981584708No Hit
GAGTTCTACAGTCCGACGA184970.12308403597990517Illumina DpnII expression Sequencing Primer (100% over 19bp)
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGG184290.12263154560597245No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC178160.11855247797037308No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGGC177540.1181399132176697No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG175510.11678909636607643No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCCCC173990.1157776472949327No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCC170310.11332887585953208No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG168720.1122708468969541No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGG166970.11110634961109783No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG165300.10999508648688071No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCC165290.10998843221667581No Hit
CGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATG164840.10968899005745564No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGC164790.10965571870643118No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCC161560.10750638943025073No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC161530.10748642661963606No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG161150.10723356435185014No Hit
CCCCCGCGGGGGCGCGCCGG160570.10684761667996634No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGG160550.10683430813955655No Hit
TAACCCAGAGGTCGATGGATCGTAACCATCCTCTGC160020.10648163181869721No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCG158350.1053703686944801No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCT157830.10502434664382566No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGG155130.10322769368850457No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGG150310.10002033544974616No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)