FastQCFastQC Report
Tue 5 Sept 2023
SRR12764802_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764802_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20194457
Total Bases642 Mbp
Sequences flagged as poor quality0
Sequence length15-44
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT5691042.8181198434798223No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG4178582.0691717534172867No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG3348741.6582471120664448No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2719521.3466665630078591No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT2696871.3354506139976925No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC2682891.3285279222907553No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGA1910950.9462745148334516No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC1873720.9278387628842906No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG1834520.9084274957232077No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG1764890.8739477372429474No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1683210.8335009948522013No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG1663200.8235923352630873No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC1541130.7631450551010112No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGT1528010.7566482228266895No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGG1448940.7174939142953931No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGG1369660.6782356168328765No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC1314560.6509509020222727No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT1264090.6259588955523786No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGA1221230.6047352498757456No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA1066350.5280409371739978No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC1020030.5051039500591672No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG1000220.4952943275474057No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC960090.4754225379766339No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGA957340.4740607781630375No Hit
TAACCCAGAGGTCGATGGATCGTAACCATCCTCTGC932460.4617405657403911No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG916880.4540255774146342No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC872240.43192050174956426No Hit
GCCGGGCGCGGTGGCGCGT855000.42338350568178185No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGA817530.4048289092397978No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT797050.3946875125189055No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA773620.38308531890706443No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGC772390.3824762408813468No Hit
TAACCCAGAGGTCGATGGATCGTAACCATCCTCTG728380.3606831320099372No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC719950.3565087191995308No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGG719270.35617199313653247No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC717540.35531532241743363No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG703850.3485362344726575No Hit
TAACCCAGAGGTCGATGGATCGAAACCATCCTCTGC691600.3424702134848191No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGG661290.32746114441205326No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA652520.32311836857014775No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG648440.3210980121921575No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC633850.3138732573992953No Hit
CGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATG627200.31058027457732584No Hit
TAATCTCAGGGTCGTGGGTTCGTGCCCCACGTTGGG609980.3020531822172787No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTC606360.30026061111719915No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGG595910.29508592382553295No Hit
TAATCCCAGGGTCGTGGGTTCGTGCCCCACGTTGGG586240.2902974811355413No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGT579870.2871431502218653No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA570500.2825032631479024No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGG568510.2815178442282454No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGT557410.2760212864351837No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGA552160.2734215631546815No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGG541960.26837067220970584No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCC539690.2672466013817554No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC537570.2661968083618193No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC518720.2568625638213496No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGC509750.2524207509020916No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGG506520.2508213021028493No Hit
TCCTTAGGTCGCTGGTTCGTTTCCGGCTCGAAGG504410.24977646093678083No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGT491950.24360645101772233No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT491390.2433291472011354No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGG478120.23675803711879945No Hit
TAACCCAGAGGTCGATGGATCGAAACCATCCTCTG471830.2336433210360645No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC462160.22885487834607288No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCT461920.22873603385324992No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA461850.22870137087617654No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG436980.21638611030739774No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG434570.21519271352530056No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGG431340.21359326472605825No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGC425650.21077565987538066No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG425400.21065186352869006No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGGC417140.20656163223403334No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG414360.20518501685883409No Hit
AGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGTG408270.20216933785345156No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGA399230.19769286195712021No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGA390780.19350854543897864No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCC388620.192438945003572No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC388070.19216659304085276No Hit
AGCAGTTGACCCGGGTTCGTTTCCCGGCCAACG379740.18804169876912263No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGA373120.18476357150875608No Hit
TAATCTCAGGGTCGTGGGTTCGGGCCCCACGTTGGG364820.18065353279862884No Hit
CGACTGCATAATTTGTGGTAGTGGGGG355080.17583042713156388No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGT351040.1738298781690441No Hit
TCCGTAGTGTAGTGGTTATCACGTTCG350420.17352286322925148No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCG346970.17181447364492147No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG339480.16810553509807172No Hit
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGG331540.1641737631271789No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT330410.16361420364013748No Hit
TAACCGAAAGGTTGGTGGTTCGTGCCCACCCAGG329580.16320319976912476No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGC324330.16060347648862258No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGG320080.15849893859488273No Hit
TAACCCAGAGGTCGATGGATCGGAACCATCCTCTGC312480.15473552965548915No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCG312320.15465629999360714No Hit
CGACAGAGTGGTTCATTTCCACCTTTCGGGCGCCA307370.15220513232913369No Hit
CGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGC295820.14648574111202892No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAG295220.14618862987997153No Hit
TTCTTGCGACCCGGGTTCGGTTCCCGGGCGGCG293080.14512893315230016No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAG290440.14382164373124764No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGG290250.1437275585077628No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC290170.14368794367682183No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAG290150.14367803996908657No Hit
CCGAAAGGTTGGTGGTTCGTGCCCACCCAGG289680.14344530283730828No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGG285040.14114764264273114No Hit
AGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCG284200.1407316869178508No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC283940.14060293871729257No Hit
TACAGTCCGACGATC282520.13989977546809007Illumina DpnII expression Sequencing Primer (100% over 15bp)
CGCCGGCCGAGGTGGGATCCCGAG282280.13978093097526711No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGG276700.1370177965171334No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTC276600.13696827797845718No Hit
CCCCCGCGGGGGCGCGCCGGC274480.13591848495852105No Hit
TCAGAAGGTTGCGTGTTCAGGTCACGTCGGGG272840.13510638092423083No Hit
CGCAGCAACCTCGGTTCGAATCCGAGTCACGG271940.13466071407614474No Hit
CTCGCCGGCCGAGGTGGGATCCCGAG271500.13444283250596933No Hit
CGCGAAAGGTCCCCGGTTCGTAACCGGGCGG267670.13254627247466966No Hit
TCCTTAGGTCGCTGGTTCGTTTCCGGCTCGAAG267450.13243733168958194No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGG259370.1284362337645424No Hit
TTCTTGCGACCCGGGTTCGATTCCCGGGCGGCG258300.12790638540070673No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG253400.1254799770055714No Hit
TAACCCAGAGGTCGATGGATCGGAACCATCCTCTG251150.12436580988535617No Hit
TTGCGACCCGGGTTCGTTTCCCGGGCGGCG248020.12281587962479013No Hit
CGCGGGAGACCGGGGTTCAATTCCCCGACGGGG246920.12227117569935156No Hit
TCCATAGTGTAGTGGTTATCACGTCTGCTTT246560.12209290896011712No Hit
CCGAAAGGTTGGTGGTTCGTGCCCACCCAGGG241450.11956251163376166No Hit
CGCGGGAGACCGGGGTTCAATTCCCCGACGGGGA235570.11665082155959926No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG231820.11479387635924054No Hit
TCTTGCGACCCGGGTTCGGTTCCCGGGCGGCG230300.11404119457136183No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGG226910.11236251611023759No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC226840.11232785313316422No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCG225470.11164944915329983No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGA224050.11094628590409734No Hit
CGGGAGGCCCGGGTTCGTTTCCCGGCCAATG220260.10906953328826816No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTC218660.10827723666944845No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGG215680.10680158421689674No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCG214990.10645990630003074No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC212780.10536554659528602No Hit
TCGCCGGCCGAGGTGGGATCCCGAG210910.10443954992204049No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGG209010.1034986976871921No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTG207500.10275096775318097No Hit
TCTTGCGACCCGGGTTCGATTCCCGGGCGGCG206390.10220131197387482No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG205980.10199828596530226No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG204980.10150310057853995No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)