FastQCFastQC Report
Tue 5 Sept 2023
SRR12764803_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764803_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10316817
Total Bases315.5 Mbp
Sequences flagged as poor quality0
Sequence length15-44
%GC70

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG3565523.4560271835780356No Hit
CAGAGTTCTACAGTCCGACGATC3146373.049748774258572Illumina DpnII expression Sequencing Primer (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC3102213.0069448745674174No Hit
GTTCTACAGTCCGACGATC2619602.5391552452660546Illumina DpnII expression Sequencing Primer (100% over 19bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2598462.518664429154845No Hit
AGAGTTCTACAGTCCGACGATC2296632.2261032642141467Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTTCTACAGTCCGACGATC1985741.9247603209400728No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC1757771.703791004531727No Hit
TCTACAGTCCGACGATC1709071.656586522761817Illumina DpnII expression Sequencing Primer (100% over 17bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCG1590661.5418127509676676No Hit
TTCTACAGTCCGACGATC1416061.3725745062648682Illumina DpnII expression Sequencing Primer (100% over 18bp)
CTACAGTCCGACGATC1110521.0764172709470372Illumina DpnII expression Sequencing Primer (100% over 16bp)
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT1022510.9911099518388278No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG987270.9569521297121002No Hit
GAGTTCTACAGTCCGACGATC986840.9565353344931872Illumina DpnII expression Sequencing Primer (100% over 21bp)
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG921780.8934732485804487No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC905970.8781487546013464No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC845870.8198943530742088No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG839310.8135358027577693No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT797600.7731066665232116No Hit
AGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGTG792980.7686285411479141No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC789080.7648483054414943No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG621120.6020461543516765No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT595310.5770287483048309No Hit
TACAGTCCGACGATC565350.5479887837498717Illumina DpnII expression Sequencing Primer (100% over 15bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC533450.5170683942537703No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT515310.49948545176288384No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG471650.4571661976751163No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGG467940.45357012729798346No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC415940.4031669845457179No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC415910.40313790580951464No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCC412650.39997801647543035No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC403750.39135132473513873No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG380000.36833065857424824No Hit
CCCCCGCGGGGGCGCGCCGGC376460.36489936770226705No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG349190.3384667964935309No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC332860.32263827108690596No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC323730.31378864236905624No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA317050.3073137771078037No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCT309760.3002476442104188No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGA301280.2920280547769724No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGT279680.2710913647106467No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG277030.26852274301269474No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG270940.262619759563439No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC270480.2621738856083228No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG269270.26100104324812584No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA268530.2602837677551128No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCCCC265640.257482516167535No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA261440.2534114930990828No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCG261170.2531497844732537No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTC257710.2497960368978145No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC246850.2392695343922452No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG240350.23296914154821202No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGG240320.23294006281200877No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGG233510.22633918969387554No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC229000.2219676863513233No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGA225400.21847823800693567No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG220250.21348638829204783No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA219390.21265279785422192No Hit
CGACTGCATAATTTGTGGTAGTGGGGG208610.20220383864519453No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT206940.20058512232988138No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG189340.18352559709065305No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT182150.17655639331394557No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC176350.17093450431465443No Hit
TAACCCAGAGGTCGATGGATCGTAACCATCCTCTGC176030.17062433112848663No Hit
TAATCTCAGGGTCGTGGGTTCGTGCCCCACGTTGGG173490.1681623314632798No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGG169810.16459533982235025No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCC168370.1631995604845952No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGG166080.16097988362108198No Hit
CAGAGTTCTACAGTCCACGATC165400.16032076560047542No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGT164010.15897345082305908No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG158710.15383620742715512No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGGC158440.15357449880132604No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGC156810.15199455413428387No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGG151620.14696393277112504No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTG149780.14518043695066027No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCC147290.14276690184579216No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC145440.14097371311325965No Hit
CGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATG143830.13941315427035295No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG141760.13740672147233007No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC138140.13389788730380697No Hit
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGG136110.13193022615405509No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCG136010.13183329703337765No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGT134340.1302145807180645No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGG133790.12968147055433862No Hit
CCCCGCGCAGGTTCGTATCCTGC133640.12953607687332247No Hit
AGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGT132910.1288284942923772No Hit
CGTCGCGCGCGCGTCCGCTGGGGGCGGGGAGCGGT132010.1279561322062803No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCT130700.1266863607254059No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG130350.12634710880303487No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG128960.12499979402561855No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG128270.12433098309294426No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGG128240.12430190435674104No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG126060.12218884952597299No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC124430.12060890485893082No Hit
TCCTTAGGTCGCTGGTTCGTTTCCGGCTCGAAGG121180.11745870843691422No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA120390.1166929683835625No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCT119120.11546196855095908No Hit
TAACCCAGAGGTCGATGGATCGAAACCATCCTCTGC116830.11324229168744585No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG116580.11299996888575227No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC115880.11232146504101023No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGT115270.11173019740487787No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGA114230.11072213454983257No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGC113170.10969468587065177No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGA112940.10947174889309368No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGG110500.1071066783485643No Hit
AGAGTTCTACAGTCCACGATC109340.10598230054870605No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCC104740.1015235609975441No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCG103690.10050580523043105No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGG103690.10050580523043105No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGG103280.10010839583565356No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)