FastQCFastQC Report
Tue 5 Sept 2023
SRR12764804_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764804_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9134078
Total Bases284.3 Mbp
Sequences flagged as poor quality0
Sequence length15-44
%GC71

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC2720282.9781659407769454No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1775441.9437539289679813No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1766851.934349586241764No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC1368111.4978085363405043No Hit
CAGAGTTCTACAGTCCGACGATC1286461.4084180143852505Illumina DpnII expression Sequencing Primer (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCG1257561.3767782582982102No Hit
GTTCTACAGTCCGACGATC1126471.2332607626078953Illumina DpnII expression Sequencing Primer (100% over 19bp)
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG1067741.1689630852725366No Hit
AGAGTTCTACAGTCCGACGATC1035531.133699537052344Illumina DpnII expression Sequencing Primer (100% over 22bp)
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG1015861.1121647964906802No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT921921.0093191671890693No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT830190.9088930486470556No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTC809120.8858255863372309No Hit
AGTTCTACAGTCCGACGATC794090.8693707235694724No Hit
TCTACAGTCCGACGATC718790.7869321895433781Illumina DpnII expression Sequencing Primer (100% over 17bp)
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC718290.7863847889190348No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC623810.6829479669431332No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC603090.6602636850703486No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTC587500.6431957336033258No Hit
TTCTACAGTCCGACGATC581390.6365064979738514Illumina DpnII expression Sequencing Primer (100% over 18bp)
TCGCCGGCCGAGGTGGGATCCCGAGGCCT573900.6283064366211893No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT558740.6117092496911019No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG520660.5700192181411194No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT516680.5656619091713471No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGG512230.5607900436146921No Hit
CTACAGTCCGACGATC497540.5447074132714873Illumina DpnII expression Sequencing Primer (100% over 16bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG441680.4835518155198587No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC438510.48008129556152246No Hit
CCCCCGCGGGGGCGCGCCGGC433650.474760561492906No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA425560.4659036193910321No Hit
GAGTTCTACAGTCCGACGATC421400.4613492461964962Illumina DpnII expression Sequencing Primer (100% over 21bp)
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT413380.45256894018203037No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT394980.4324245972061986No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC372810.40815285352281866No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG364300.39883609489649635No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGG354310.38789903042211815No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG346510.37935958068236336No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC338590.3706887547927662No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC333370.36497389227462257No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA329230.3604414151050604No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG327920.3590072254692811No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCC305530.3344946255111901No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC289880.31736098596924617No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT289410.3168464293823635No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCC280630.30723407441889594No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGA278640.30505541993400975No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGC272180.2979830038674949No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG267890.29328630651062976No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCT267870.29326441048565605No Hit
TACAGTCCGACGATC259660.2842760922339398Illumina DpnII expression Sequencing Primer (100% over 15bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGG252250.2761636149811727No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC243050.26609144349325675No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGT239630.26234722322274895No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGG234380.2565995166671447No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG227820.24941762047576121No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC221050.24200581602215354No Hit
CGACTGCATAATTTGTGGTAGTGGGGG219290.24007896582446528No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA218490.23920312482551606No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGG217980.23864477618868596No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCCCC211370.23140813993486808No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA203110.2223650816207175No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC199820.21876318551253887No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG195760.21431829244287162No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG195740.2142963964178979No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC190140.20816550942525341No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC185450.20303089156891366No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG183420.20080844503408005No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGA181650.19887064682390493No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGC169770.18586440798950918No Hit
ACTGGACGCCTCGCGGCGCCCATCTCCGCC165450.18113486659518344No Hit
ACCTCGGCCGGCGAGCGCGCCGGCCTTCACCTTC164310.17988679317168083No Hit
TAATCTCAGGGTCGTGGGTTCGTGCCCCACGTTGGG163260.17873725186055997No Hit
TAACCCAGAGGTCGATGGATCGTAACCATCCTCTGC161390.17668997352551621No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCC160500.1757156004141852No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGG159240.1743361508408402No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCG158650.17369021810411517No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG157050.17193853610621676No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCC154460.1691030008721187No Hit
ACGCGGCGCTCCCCCGGGGAGGGGGGAGGAC151860.16625651762553373No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCG150070.16429682339038487No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC148240.16229333710528857No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTC144480.1581768844102273No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTG142460.15596538588788053No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGG139360.15257150201695233No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGC139060.15224306164234638No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGG135380.14821419304718003No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGGC133330.14596985048737268No Hit
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGG133050.14566330613774045No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC130840.14324379537814325No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA129760.14206141002956182No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG128390.14056153231886132No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCG127490.13957621119504343No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC126110.13806538547185607No Hit
TCCTTAGGTCGCTGGTTCGTTTCCGGCTCGAAGG125170.13703627229809073No Hit
AGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGTG123220.13490140986315205No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG123010.13467150160092786No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG122440.13404746488917654No Hit
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCC121310.1328103394781608No Hit
TAACCCAGAGGTCGATGGATCGAAACCATCCTCTGC120970.1324381070536074No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGT120120.13150752599222384No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGC115120.1260335197487913No Hit
TCGACTGCATAATTTGTGGTAGTGGGG114730.12560654726180354No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGC111900.12250825972802072No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG110190.12063614959276678No Hit
CGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATG108820.11913627188206626No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGT108470.118753091445026No Hit
TAACCCAGAGGTCGATGGATCGTAACCATCCTCTG108330.11859981927020986No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGAC107400.11758165410893141No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGG106470.11656348894765296No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGG106440.11653064491019237No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG104540.11445052253768798No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG103550.11336666930148832No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG102400.11210764786549884No Hit
TAATCCCAGGGTCGTGGGTTCGTGCCCCACGTTGGG102090.11176825947840603No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG101710.11135223500390516No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGG101020.11059682214231145No Hit
AGGCGTAGCCCCGGGAGGAACCCGGGGCCGC100510.11003847350548135No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGG99820.10928306064388765No Hit
CGACTGCATAATTTGTGGTAGTGGGG99270.10868091995711007No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGA97920.10720293827138327No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC97880.10715914622143581No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGG97170.1063818373348684No Hit
AGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTC96950.10614098106015736No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGG96620.10577969664809081No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGA93670.10255003296446559No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC92820.10161945190308207No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGC92640.1014223876783185No Hit
TAATCTCAGGGTCGTGGGTTCGGGCCCCACGTTGGG92540.10131290755344984No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCG92290.10103920724127821No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)