FastQCFastQC Report
Tue 5 Sept 2023
SRR12764809_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764809_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7101140
Total Bases227.5 Mbp
Sequences flagged as poor quality0
Sequence length15-42
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGGATAGCTCAGTCGGTAGAGCATCAGA3480884.901860827979733No Hit
CCCGCGAGGGGGGCCCGGGC3030924.268216089247642No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGA1207901.7009944882089356No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGA1120901.5784789484505304No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTC928821.307987168257491No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC857411.2074258499339543No Hit
GGGGGGTCTCCCCCGCGGGGGCGCGCCGGC654580.9217956553454797No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT632400.8905612338300611No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG584780.8235015786197709No Hit
CTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGATCG537560.7570052132474504No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC506080.7126743029992367No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC504700.7107309530582413No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGC498430.7019013848480666No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGA458150.6451780981645201No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG444650.6261670661330434No Hit
CCCGCGAGGGGGGCCCGGG431260.6073109388070085No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT418360.5891448415324864No Hit
CTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGATCG334910.4716284990860622No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC319570.45002633379992507No Hit
GGGGGTGTAGCTCAGTGGTAGAGCGTGCTT318030.44785766792374176No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGA316150.4452102056852843No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCG311800.4390844286973641No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGA295510.41614445004604894No Hit
CCGCGAGGGGGGCCCGGGC286250.40310429029705086No Hit
AGGTCGCTGGTTCGTTTCCGGCTCGAAGGA280700.3952886437952216No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGC250160.35228146466623667No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG237440.33436884781880094No Hit
TCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGA235440.3315523986289525No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTCG223890.3152874045575781No Hit
CCCGCGAGGGGGGCCCGG221080.31133029344584107No Hit
TTCCGTAGTGTAGTGGTTATCACGTTCGC216270.30455673314425574No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC214410.3019374353976967No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC208160.2931360316794205No Hit
CCCGGATAGCTCAGTCGGTAGAGCATCAGA197440.2780398640218331No Hit
GGGGGGTCTCCCCCGCGGGGGCGCGCCGGCG190920.2688582396629274No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA190140.26775982447888647No Hit
GGTAGCGTGGCCGAGCGGTCTAAGGCTCTGGATT188470.2654080894053631No Hit
TAATCTGAGGGTCCAGGGTTCAGGTCCCTGTTCGGG179460.2527199858050961No Hit
GGTAGCGTGGCCGAGCGGTCTAAGGCTGGATT177820.2504104974694204No Hit
TAATCTGAGGGTCCAGGGTTCAGGTCCCTGTTCGG167940.23649723847156937No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTA167930.2364831562256201No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA167440.23579312617410725No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCT166440.23438490157918307No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCG164750.23200500201376117No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA154950.21820440098350405No Hit
ATCCATTGGGGTTTCCCCGCGCAGGT154630.2177537691131283No Hit
TCTAAAGGTCCCTGGTTCGTTCCCGGGTTTCGG153030.2155006097612496No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG149240.2101634385464869No Hit
CTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGG144930.2040939905423636No Hit
TGGGAAACTCGACTGCATAATTTGTGGTAGTGGGGGA142510.2006860870226471No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCG140100.19729226574887976No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGT139470.19640508425407752No Hit
GGGGATGTAGCTCAGTGGTAGAGCATGCTT135300.1905327876932436No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGT135250.1904623764634974No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG134360.18920905657401488No Hit
TAATCTCAGGGTCGTGGGTTCGTGCCCCACGTTGGGC128510.18097094269370834No Hit
GGTAGCGTGGCCGAGCGGTCTAAGGCCTGGATT125350.17652095297374787No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGT120020.1690151158828019No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGACT119690.16855040176647693No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGC116680.1643116457357551No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGC115060.1620303218919779No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTT104370.14697640097223824No Hit
TAATCTCAGGGTCGTGGGTTCGGGCCCCACGTTGGGC102650.14455425466896865No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTATT100400.1413857493303892No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGG100060.14090695296811498No Hit
GGTAGCGTGGCCGAGCGGTCTAAGGCGCTGGATT98820.139160754470409No Hit
AGCTGGGGATTGTGGGTTCGTGTCCCATCTGGG98360.13851297115674385No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT96680.1361471538372712No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGCCT96250.1355416172614538No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGA95140.13397848796108794No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC92030.12959890947087369No Hit
GTCAGGATGGCCGAGCGGTCTAAGGCTGCGTT91960.129500333749229No Hit
AGCTGGGGATTGTGGGTTCGGGTCCCATCTGGG90460.12738799685684268No Hit
TAATCTGAGGGTCCAGGGTTCAGGTCCCTGTTCGGGCG89720.1263459106565988No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCGC89270.1257122095888829No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT87610.12337455676130875No Hit
GGGGGTCTCCCCCGCGGGGGCGCGCCGGC87500.12321965205586709No Hit
TAAGCCAGGGATTGTGGGTTCGTGTCCCATCTGGGG85730.12072709452285126No Hit
GAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC85060.11978358404425204No Hit
TAACCGAAAGGTTGGTGGTTCGTGCCCACCCAGG83370.11740368447883016No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGC82250.11582647293251506No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCC81760.1151364428810022No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGG81090.114192932402403No Hit
CCCCGCGCAGGTTCGTATCCTGCCGACTACG79180.11150322342609778No Hit
GCCGAAATAGCTCAGTTGGGAGAGCTTTAGA77740.10947538000940693No Hit
TTCCGTAGTGTAGTGGTTATCACGTTCGCCT77080.10854595177675697No Hit
TAAGCCAGGGATTGTGGGTTCGGGTCCCATCTGGGG76570.10782775723334563No Hit
CGCGGGTCGGGGGGCGGGGCGGACTGT75970.10698282247639111No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA75260.10598298301399493No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCC73440.1034200142512329No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCG73410.10337776751338516No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC73340.10327919179174047No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG72700.10237792805098898No Hit
GTCAGGATGGCCGAGCGGTCTAAGGCTGCG72150.10160340452378068No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)