FastQCFastQC Report
Tue 5 Sept 2023
SRR12764811_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764811_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21586713
Total Bases681 Mbp
Sequences flagged as poor quality0
Sequence length15-42
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGGATAGCTCAGTCGGTAGAGCATCAGA12162585.634289944930476No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTC7848733.635907884632551No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGA4574372.1190674096607482No Hit
CCCGCGAGGGGGGCCCGGGC3480811.612478009041951No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG3314791.5355695885705247No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGA3303201.530200545122363No Hit
GGGGGGTCTCCCCCGCGGGGGCGCGCCGGC2480251.1489706654273857No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT1868060.8653749183583439No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT1864550.8637489181423776No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA1781180.825127938653745No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT1680930.7786873341948818No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG1626440.7534449547737999No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC1578190.7310932424033247No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTCG1520620.7044240593739306No Hit
TGGGAAACTCGACTGCATAATTTGTGGTAGTGGGGGA1409300.6528553003877895No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC1396870.6470971286828152No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC1395530.6464763764636144No Hit
GGGGGTGTAGCTCAGTGGTAGAGCGTGCTT1273620.5900018219540881No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGA1216640.5636059552003123No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGC939150.43505928855402853No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGT924360.4282078517465813No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG917840.4251874752770373No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGC856570.3968042749259695No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGC855420.39627153981247637No Hit
CCCGCGAGGGGGGCCCGGG815450.377755520259152No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGA791220.3665310230418128No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC766390.35502857706960755No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA714720.331092556796396No Hit
GGGGATGTAGCTCAGTGGTAGAGCATGCTT714610.33104159952467055No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGA714180.33084240291701655No Hit
GCATTGGTGGTTCAGTGGTAGAATTCT645090.298836603794195No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTA639540.29626557781168444No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC631490.29253643201723206No Hit
TCTAAAGGTCCCTGGTTCGTTCCCGGGTTTCGG629430.29158214129219207No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGA614990.2848928412584167No Hit
TGGGTGGTTCAGTGGTAGAATTCTCGC578980.2682112834872081No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG577000.26729405259615024No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA570890.2644636077757646No Hit
CGCGGGTCGGGGGGCGGGGCGGACTGT549050.2543462730986418No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC534690.24769403289884848No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCG530410.24571133178080423No Hit
CCGCGAGGGGGGCCCGGGC524530.2429874339831173No Hit
CTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGATCG519850.2408194336951624No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCG511260.23684013402133064No Hit
AGGTCGCTGGTTCGTTTCCGGCTCGAAGGA503110.2330646634344006No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG496330.22992384250441464No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGT481680.2231372604064361No Hit
GGGGGTCTCCCCCGCGGGGGCGCGCCGGC480670.22266938000241165No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGC457970.2121536521099808No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTC442010.2047602152305448No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGT425340.1970378723245174No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCG408910.18942670892043637No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGT408290.18913949520707485No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGG398180.1844560586875825No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGT387770.17963364779065716No Hit
GGGGGGTCTCCCCCGCGGGGGCGCGCCGGCG380950.1764742969436801No Hit
CATGGGTGGTTCAGTGGTAGAATTCTCGC379130.17563118572058656No Hit
ACTCGACTGCATAATTTGTGGTAGTGGGGGA367430.1702111850006993No Hit
CCCGCGAGGGGGGCCCGG348080.16124733765627033No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCG345370.1599919357801255No Hit
CTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGATCG342840.15881991853044047No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGTCG336720.1559848412308071No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCG335180.15527143942665103No Hit
TCTTGGTCTAGGGGTATGATTCTCGCTT334850.15511856761147472No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA326160.15109294314516528No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCG321600.14898053260818356No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGT318940.14774829312827759No Hit
TCGTTGGTCTAGGGGTATGATTCTCGCTT318180.14739622470544728No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGACT301210.13953490742198685No Hit
CGGGTCGGGGGGCGGGGCGGACTGT300030.13898827487075036No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC299010.13851576198747811No Hit
TCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGA298860.13844627479876162No Hit
TAATCTGAGGGTCCAGGGTTCAGGTCCCTGTTCGGG298110.1380988388551791No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC291290.13493948800820207No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC290350.13450403495891197No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAG286880.13289656465993688No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTT285550.1322804449199839No Hit
CCCAGGTGGCCCGGGTTCGACTCCCGGTATGGG284880.1319700688103835No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCG284420.1317569747649862No Hit
CATTGGTGGTTCAGTGGTAGAATTCTCGC274010.12693456386806087No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG272310.12614704239594052No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGG271830.12592468339204768No Hit
TCCCTGTGGTCTAGTGGTTAGGATT270710.1254058457162978No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGGC268140.1242152985496217No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG263670.12214458032586989No Hit
TTCCGTAGTGTAGTGGTTATCACGTTCGC259740.1203240159814975No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTG255010.11813285329730376No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGG253730.1175398959535896No Hit
ATTGGTGGTTCAGTGGTAGAATTCTCGC251190.1163632462246568No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCG250060.11583977606965914No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCC244480.11325485264940521No Hit
TAATCTCAGGGTCGTGGGTTCGTGCCCCACGTTGGGC242780.11246733117728486No Hit
TCCCTGGTGGTCTAGTGGTTAGGAT233530.10818228787310046No Hit
TAATCTGAGGGTCCAGGGTTCAGGTCCCTGTTCGG232400.10765881771810279No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCG232170.10755227069540416No Hit
CTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGG222790.10320700516099882No Hit
TAATCTCAGGGTCGTGGGTTCGTGCCCCACGTTGGG218040.10100657751830953No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)