FastQCFastQC Report
Tue 5 Sept 2023
SRR12764812_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12764812_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24724089
Total Bases756.6 Mbp
Sequences flagged as poor quality0
Sequence length15-42
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGCGAGGGGGGCCCGGGC8777433.550153051139721No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGA7087132.8664878208454923No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGA5982042.419518874891609No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGA5284652.1374498368777104No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC3744121.514361156036932No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTC3582821.4491211384977623No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG3118851.2614620502296363No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG2712821.097237596903975No Hit
GGGGGGTCTCCCCCGCGGGGGCGCGCCGGC2556861.0341574162752771No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG2088020.8445285891019079No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT1980690.8011174850567802No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC1952270.7896226226980496No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC1944180.7863505102250683No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTCG1891960.7652294084526229No Hit
CCGCGAGGGGGGCCCGGGC1747090.7066347318196436No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT1631290.6597978190419878No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG1478880.598153485048529No Hit
CCCGCGAGGGGGGCCCGGG1438040.5816351817856666No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC1402850.5674020992239592No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGC1386240.5606839548264043No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGC1249890.5055353101179987No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA1152610.4661890676740405No Hit
GGGGGTGTAGCTCAGTGGTAGAGCGTGCTT1115460.4511632359841449No Hit
CCCCCGCGGGGGCGCGCCGGC1096890.44365234245840157No Hit
TGGGTGGTTCAGTGGTAGAATTCTCGC1036140.41908116412297336No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGA986660.39906829327462784No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA979320.39609952868233084No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG975190.3944290930193626No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC890040.35998899696567177No Hit
CCCGCGAGGGGGGCCCGG873260.35320209371516176No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGA853920.345379763031916No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC852140.3446598173950919No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGC839040.33936134107913946No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGC837940.3389164308541358No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTA759490.3071862425345581No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGC727710.294332381670362No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA698700.28259888564549335No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT693150.2803541113284295No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGA688650.2785340240443237No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA667350.26991894423288965No Hit
CATGGGTGGTTCAGTGGTAGAATTCTCGC659330.2666751442287722No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGA639300.25857373349529683No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGT590890.2389936389567276No Hit
TTCTTGCGACCCGGGTTCGATTCCCGGGCGGCGC555340.22461494941229176No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC538780.21791702820678246No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCG536070.21682093119790988No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTC533820.21591088755585694No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGG526650.21301088181651506No Hit
GGGGGTCTCCCCCGCGGGGGCGCGCCGGC525520.21255383767628402No Hit
CCCCCGCGGGGGCGCGCCGG506430.20483262295326632No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG505940.20463443567121925No Hit
AGGTCGCTGGTTCGTTTCCGGCTCGAAGGA497940.20139872494392008No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC496260.20071922569118725No Hit
TAATCTGAGGGTCCAGGGTTCAGGTCCCTGTTCGGG494960.2001934226980011No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAG464860.1880190610865379No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT441890.1787285266607801No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGT438180.1772279658109951No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCG436080.17637859174507906No Hit
GGGGATGTAGCTCAGTGGTAGAGCATGCTT428200.17319141667868934No Hit
CTGTCCCCGCCGGCGGGC421610.17052599996707665No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC407530.16483114908703006No Hit
TTCTTGCGACCCGGGTTCGATTCCCGGGCGGCG400300.16190687551723343No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG397540.1607905553163152No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCG394790.15967827975380608No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGT383500.1551118829899051No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGG383420.15507952588263213No Hit
TAATCTCAGGGTCGTGGGTTCGTGCCCCACGTTGGGC382360.15465079421126499No Hit
TTCCGTAGTGTAGTGGTTATCACGTTCGC379280.15340504558125478No Hit
CGGGGGGCGGGGCGGACTGT376400.15224018971942707No Hit
ATGGGTGGTTCAGTGGTAGAATTCTCGC368860.14919053235894758No Hit
GGGGGGTCTCCCCCGCGGGGGCGCGCCGGCG367040.148454408168487No Hit
TCTAAAGGTCCCTGGTTCGTTCCCGGGTTTCGG366780.1483492475698498No Hit
TAATCTGAGGGTCCAGGGTTCAGGTCCCTGTTCGG366300.14815510492621184No Hit
TCTTGGTCTAGGGGTATGATTCTCGCTT365360.14777490891575418No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG365160.14769401614757172No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGT361880.14636737474937903No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCG360670.14587797350187504No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG353720.14306694980753387No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA349640.14141673733661128No Hit
TCGTTGGTCTAGGGGTATGATTCTCGCTT336990.13630026974906942No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGC335620.13574615428701942No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGC326570.13208575652676222No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGCCT323850.1309856148794805No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCG321170.12990165178583526No Hit
TGCGAGAGGTCCCGGGTTCAAATCCCGGACGAG320070.12945674156083162No Hit
GCATTGGTGGTTCAGTGGTAGAATTCT319840.1293637148774218No Hit
TGCGAGAGGTCCCGGGTTCATATCCCGGACGAG314130.12705422634581195No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCG310110.12542828170534412No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCG309380.12513302310147809No Hit
CGCGGGTCGGGGGGCGGGGCGGACTGT307400.12433218469647153No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTT305290.12347876599214636No Hit
GCGCCGCTGGTGTAGTGGTATCATGCA304060.12298127546782411No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCG299500.12113692035326358No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTA296050.11974152010211579No Hit
CGTCGCGCGCGCGTCCGCTGGGGGCGGGGAGCGGT288090.11652198792845309No Hit
GATCGGAAGAGCACACGTCTGAACTCCAG287830.11641682732981588Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTG284940.11524792682957904No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGC284680.11514276623094183No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGG284190.11494457894889473No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGT280070.11327818792433565No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC279940.11322560762501703No Hit
TCCCTGTGGTCTAGTGGTTAGGATT279220.11293439365956011No Hit
TAATCTGAGGGTCCAGGGTTCAGGTCCCTGTTCGGGC275760.11153494877000321No Hit
TCCCTGGTGGTCTAGTGGTTAGGAT272010.11001820936658172No Hit
TCTTGCGACCCGGGTTCGATTCCCGGGCGGCGC271900.10997371834408134No Hit
TGCGAGAGGTCCCGGGTTCAGATCCCGGACGAG270620.10945600462771347No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG263770.10668542731746354No Hit
TTGCGACCCGGGTTCGTTTCCCGGGCGGCGC262870.10632140986064238No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGG260380.10531429489677052No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA260190.10523744676699716No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTAT259170.10482489364926652No Hit
CATTGGTGGTTCAGTGGTAGAATTCTCGC258630.10460648317517382No Hit
GGGGGGTCTCCCCCGCGGGGGCGCGCCGG255720.10342949339811874No Hit
AGACCGGGGTTCGATTCCCCGACGGGGA255320.10326770786175377No Hit
ATTGGTGGTTCAGTGGTAGAATTCTCGC251290.10163771858287682No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)