FastQCFastQC Report
Wed 6 Sept 2023
SRR12765221_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12765221_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40543028
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATG865770.21354349754044025RNA PCR Primer, Index 11 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)