FastQCFastQC Report
Wed 6 Sept 2023
SRR12790134_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12790134_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36695449
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAAAA18826395.130442742368406No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAA8762812.3879827713785433No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAAA6865161.8708477991371628No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAAAAAAAAAA6853571.8676893693275152No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA5858361.5964813511343057No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAA5650221.5397604209720939No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAAAAAAAAATC3706531.0100789337664189No Hit
ATGAGAGGTGTAGAATAAGTGGGAGGCCCAAAAAAAAAAAAAAAAAAAA3129090.8527188207998218No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAAAAAAAATCG2915180.7944254885667157No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA2307950.6289472026899031No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAAAAAAAAAAA2225750.6065466047301942No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAAAAAAAAAAT2001540.5454463849181952No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAAA1982130.5401569006554464No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAAAAAAATCGT1831200.49902645965716347No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAAAAAAATCG1798800.49019702688472344No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAAAA1785740.48663800244003014No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAAAAAAAAAAC1622460.44214202148064735No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCACAAAAAAAAAAAAAAAAAA1558490.4247093420222219No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAA1544210.4208178512817761No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAAAAAAAAAAA1472240.40120506496595804No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAAAAAATCGT1455710.39670041917187054No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAAA1451380.39552043633530687No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAA1420150.3870098441907605No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTTCAAAAAAAAAAAAAAAAAAAA1242050.3384752152780581No Hit
ATGAGAGGTGTAGAATAAGTGGGAGGCCCAAAAAAAAAAAAAAAAAAAT1169230.318630792608642No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAAAAAAAAAT1150960.3136519735730717No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA1146930.3125537447436602No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAAAAAAAAAAAAAAAAA1081970.2948512770616324No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAAA1062960.28967079814175317No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAAAAAAAAA911640.24843407693417244No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAAAAAAAAA782040.21311634584441247No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAAAT764840.2084291161010184No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAAAAAAAATC741380.20203595274171465No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAAAAT735690.20048535173939416No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAAAAAAAAATT724930.19755310801620116No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAAAAAAATCT693980.18911881961166355No Hit
ATGTGGCGTACGGAAGACCCGCTCCCCGGCGCAAAAAAAAAAAAAAAAA683710.18632010743348582No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAAAAAAAAA682470.18598219087058998No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAAAAAAAATCT674730.18387293748606265No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAAAAAAAAA607800.1656336184904019No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAAAAAAAAACG606130.1651785211839212No Hit
ACCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAAA550490.15001587799075575No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAAAAA546070.14881136895204633No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAAAA540650.14733434655616284No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACAAAAAAAAAAAAAAAAAA517370.14099023560115043No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAAAAAAAA507740.1383659319715641No Hit
ACAACTTCTTAGAGGGACAAGTGGCGTTCAAAAAAAAAAAAAAAAAAAA494220.13468155138257062No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTTCAAAAAAAAAAAAAAAAAAAT493390.13445536529611615No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCAAAAAAAAAAAAAAAAAAAA489380.13336258673384813No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAAAAAAAA438930.1196142878644161No Hit
AGCGAGCGCACGGGGTCGGCGGCGACAAAAAAAAAAAAAAAAAAAATCG433260.11806913712923911No Hit
CCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAAAAAAAAAAAAAAAA432760.11793288045065208No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAAAAAAAATC421130.1147635501067176No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAAATC403250.10989101128044516No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAAAAAAAA397950.10844669048742257No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAAT379570.1034378949825631No Hit
ATGACCCGCCGGGCAGCTTCCGGGAAACCAAAAAAAAAAAAAAAAAAAA377660.10291739447036061No Hit
TCTAACCCGTTCCCCTTATCTGGGAAGGAGACAAAAAAAAAAAAAAAAA371750.10130684052946184No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)