FastQCFastQC Report
Wed 6 Sept 2023
SRR12790137.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12790137.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22078324
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA1041360.4716662369842928No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAA735250.3330189374881898No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAAA549480.24887758690378853No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAA530860.24044397572931714No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAAA430320.19490609885061927No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAA409010.18525409809186602No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA320740.14527370827604486No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAAAAAAATGG296480.13428555537096024No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAAAAAAAAA271330.12289429215732135No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA267200.12102367915245742No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAAAAAAATCG264100.11961958706648204No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAATG222980.10099498494541524No Hit

[FAIL]Adapter Content

Adapter graph